Aedes aegypti Males as Vehicles for Insecticide Delivery

Brelsfoard, Corey L.; Mains, James W.; Mulligan, Steve; Cornel, Anthony J.; Holeman, Jodi; Kluh, Susanne; Leal, Andréa L.; Hribar, Lawrence J.; Morales, Harold D.; Posey, Tanya; Dobson, Stephen L.

doi:10.3390/insects10080230

Cited by 18 publications

(11 citation statements)

References 28 publications

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“…aegypti ( Moyes et al., 2017 ). In response, novel methods of Aedes control are under development and the most promising options are: 1) transinfection with Wolbachia ( Moreira et al., 2009 ), 2) incompatible insect treatment ( Marris, 2017 ; Ritchie, 2018 ), 3) sterile insect treatment ( Lees et al., 2015 ), 4) the use of adult mosquitoes to transfer insecticides ( Devine et al., 2009 ; Mains et al., 2015 ; Brelsfoard et al., 2019 ), biological control using Bacillus thuringiensis and oomyceto Leptolegnia chapmanii ( Rodríguez-Pérez et al., 2012 ), and entomopathogenic fungi, such as Metarhizium anisopliae and Beauveria bassiana ( Scholte et al., 2007 ; Paula et al., 2008 ; Reyes-Villanueva et al., 2011 ; García-Munguía et al., 2011 ).…”

Section: Introductionmentioning

confidence: 99%

Estimating Contact Rates Between Metarhizium anisopliae–Exposed Males With Female Aedes aegypti

Reyes-Villanueva

Russell

Rodríguez‐Pérez

2021

Front. Cell. Infect. Microbiol.

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IntroductionEffective control of Aedes aegypti will reduce the frequency and severity of outbreaks of dengue, chikungunya, and Zika; however, control programs are increasingly threatened by the rapid development of insecticide resistance. Thus, there is an urgent need for novel vector control tools, such as auto-dissemination of the entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana. The aim of this study was to estimate contact rates of M. anisopliae-exposed males with wild female Ae. aegypti. As a control the contact rates of untreated males with wild females was contrasted.MethodsThe study was conducted in Reynosa, Mexico. The treatment and control households (n = 15 per group) were geographically separated by an arid and hot area that naturally prevented the flight of males between arms. In each control household, 40 M. anisopliae-exposed male Ae. aegypti were released per week for 8 weeks (specimens were exposed to a concentration of 5.96 × 107 conidia/cm2 for 24 h; n = 4,800 males). In each control household, 40 untreated males were released per week for 8 weeks (n = 4,800 males). All specimens were dust-marked prior to release. Mosquito abundance was monitored with human landing collections, and captured Ae. aegypti were examined for any dust-marking.ResultsIn the treatment households, the contact rate of Ae. aegypti females with marked, fungus-treated males was 14% (n = 29 females marked from 197). Where in the control households, the contact rate of females with marked, untreated males was only 6% (n = 22 marked from 365). In the treatment households the recapture rate of released males was at 5% and higher than that for the control households (which was 2%). Auto-dissemination of M. anisopliae from infected males to female Ae. aegypti was demonstrated through the recovery of an infected female from the floor of a household.ConclusionsOverall, the contact rate between M. anisopliae-infected males with the natural female population was 60% higher than for the control group of healthy males. The results provide further support to the release of fungus-exposed males as a potentially useful strategy against Ae. aegypti, though further research is required.

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Section: Introductionmentioning

confidence: 99%

Estimating Contact Rates Between Metarhizium anisopliae–Exposed Males With Female Aedes aegypti

Reyes-Villanueva

Russell

Rodríguez‐Pérez

2021

Front. Cell. Infect. Microbiol.

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“…Jones [ 23 ] also demonstrated sterilization of pupae, although in many cases a higher dose (20,000–30,000 rad) was needed to prevent males from recovering fertility. A potential advantage to irradiating pupae is that fewer adverse side effects might be associated with transporting pupae as compared to the more fragile adult stages, similar to reports from shipping adult mosquitoes [ 35 ]. Even though C. sonorensis were exposed to relatively high amounts of radiation, little to no somatic damage was observed [ 23 , 24 ].…”

Section: Management Toolsmentioning

confidence: 86%

Next-generation tools to control biting midge populations and reduce pathogen transmission

et al. 2021

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Biting midges of the genus Culicoides transmit disease-causing agents resulting in a significant economic impact on livestock industries in many parts of the world. Localized control efforts, such as removal of larval habitat or pesticide application, can be logistically difficult, expensive and ineffective if not instituted and maintained properly. With these limitations, a population-level approach to the management of Culicoides midges should be investigated as a means to replace or supplement existing control strategies. Next-generation control methods such as Wolbachia- and genetic-based population suppression and replacement are being investigated in several vector species. Here we assess the feasibility and applicability of these approaches for use against biting midges. We also discuss the technical and logistical hurdles needing to be addressed for each method to be successful, as well as emphasize the importance of addressing community engagement and involving stakeholders in the investigation and development of these approaches.

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“…Apart from increased resistance to the four existing families of adulticides, namely pyrethroids, organophosphates, carbamates and organochlorines [ 13 , 14 ], the control of Ae. aegypti with larvicides is hindered by a reduced susceptibility [ 15 ], international scrutiny and deregulation due to toxicity against biodiversity [ 16 ] and the existence of inconspicuous larval habitats [ 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

Aquatain® causes anti-oviposition, egg retention and oocyte melanization and triggers female death in Aedes aegypti

et al. 2022

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Background In arboviral disease systems where the virus can be transmitted from male to female vectors and from one generation to the next, targeting the female (especially when she is gravid) can help alter the persistence of the virus in nature and its transmission. A typical example is Aedes aegypti, which has become unmanageable due to the development of insecticide resistance. Despite evidence that monomolecular surface films prevent the selection of genetic resistance, their potential in Aedes vector control remains largely unexplored. Methods We examined the oviposition, egg retention, oocyte melanization, and female mortality of the Cayman Islands strain of Ae. aegypti, using choice (balanced and unbalanced) and no-choice bioassays involving Aquatain® Mosquito Formulation (AMF; Aquatain Products Pty Ltd.), a polydimethylsiloxane–based liquid used for mosquito control. Results When presented with similar opportunities to oviposit in two sites treated with AMF and two other sites with untreated water (control), egg deposition rates were significantly higher in the untreated water sites than in the AMF-treated sites (P < 0.05). We also observed a matching pattern of egg deposition preference in environments with more options in terms of AMF-treated sites. Females laid significantly more eggs when water was the only available medium than when all sites were treated with AMF (P < 0.05). Also, significantly more mature eggs were withheld in the AMF no-choice environment than in the no-choice test involving only water (P < 0.05). Internal oocyte melanization was not observed in females from the oviposition arenas with the lowest AMF presence (equal-choice and water-based no-choice); in contrast, this physiological response intensified as the number of AMF-treated sites increased. Female death occurred at high rates in AMF-treated environments, and this response increased with the increasing presence of such egg deposition sites. Conclusions This study demonstrated that AMF acted as a deterrent signal to ovipositing Ae. aegypti and as an indirect adulticide. These results suggest that AMF may be a promising control tool against the dengue vector, and this warrants further evaluation under field settings. Graphical Abstract

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Aedes aegypti Males as Vehicles for Insecticide Delivery

Cited by 18 publications

References 28 publications

Estimating Contact Rates Between Metarhizium anisopliae–Exposed Males With Female Aedes aegypti

Estimating Contact Rates Between Metarhizium anisopliae–Exposed Males With Female Aedes aegypti

Next-generation tools to control biting midge populations and reduce pathogen transmission

Aquatain® causes anti-oviposition, egg retention and oocyte melanization and triggers female death in Aedes aegypti

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