Adverse impacts of high-density microplastics on juvenile growth and behaviour of the endangered tri-spine horseshoe crab Tachypleus tridentatus

Fang, Jingzhong; Tse, Tsz Wan; Maboloc, Elizaldy A.; Leung, Ryan Kar-Long; Leung, Matthew Ming-Lok; Wong, Max Wang-Tang; Chui, Apple Pui-Yi; Wang, Youji; Hu, Menghong; Kwan, Kit Yue; Cheung, Siu Gin

doi:10.1016/j.marpolbul.2022.114535

Cited by 5 publications

(3 citation statements)

References 53 publications

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“…To compensate for the limited spatial resolution of this imaging system, a particle analyzer is used to detect micron-size MP samples with more screening parameters 52 . The particle analyzer is used to analyze ground MPs, enabling high-throughput analysis of the placed samples.…”

Section: Resultsmentioning

confidence: 99%

High-throughput microplastic assessment using polarization holographic imaging

Li,

Zhu,

Huang

et al. 2024

Sci Rep

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Microplastic (MP) pollution has emerged as a global environmental concern due to its ubiquity and harmful impacts on ecosystems and human health. MP assessment has therefore become increasingly necessary and common in environmental and experimental samples. Microscopy and spectroscopy are widely employed for the physical and chemical characterization of MPs. However, these analytical methods often require time-consuming pretreatments of samples or expensive instrumentation. In this work, we develop a portable and cost-effective polarization holographic imaging system that prominently incorporates deep learning techniques, enabling efficient, high-throughput detection and dynamic analysis of MPs in aqueous environments. The integration enhances the identification and classification of MPs, eliminating the need for extensive sample preparation. The system simultaneously captures holographic interference patterns and polarization states, allowing for multimodal information acquisition to facilitate rapid MP detection. The characteristics of light waves are registered, and birefringence features are leveraged to classify the material composition and structures of MPs. Furthermore, the system automates real-time counting and morphological measurements of various materials, including MP sheets and additional natural substances. This innovative approach significantly improves the dynamic monitoring of MPs and provides valuable information for their effective filtration and management.

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Section: Resultsmentioning

confidence: 99%

High-throughput microplastic assessment using polarization holographic imaging

Li,

Zhu,

Huang

et al. 2024

Sci Rep

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“…The crab X. testudinatus is omnivorous and a scavenger, eating a variety of food, including microbes and different organic debris, according to previous studies (Tseng et al, 2020). Fang et al (2023) reported that by intaking and absorbing suspended particles in Kueishantao waters, vent crabs absorb polycyclic aromatic hydrocarbons.…”

Section: Concerns Regarding the Impact Of Mp Particles On The Health Ofmentioning

confidence: 99%

“…Despite the fact that the microbiota of X. testudinatus were the subject of some studies as yet (Wang et al, 2014;Ho et al, 2015), little is known about the negative repercussions of MPs on HV species and their microbiota.Our attempt to understand microbial life, such as microbial community composition, functional potential, and metabolic activities, has recently been greatly expanded due to the recent revolution in microbiology provided by culture-independent analyses and bioinformatic approaches (Ho et al, 2015;Alvarez-Yela et al, 2019). Previous research has shown that MPs and nanoplastics are capable of having a variety of negative effects on aquatic organisms, including physical damage (Yuan et al, 2022;Fang et al, 2023). The present study investigated the fate and impact of polyethylene microplastics (PE-MPs) on X. testudinatus and its microbiota determined by applying culture-independent analyses.…”

Section: Introductionmentioning

confidence: 99%

Bacterial community responses of the hydrothermal vent crab Xenograpsus testudinatus fed on microplastics

Muthu,

Lee,

Girija

et al. 2023

Front. Ecol. Evol.

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Microplastics (MPs) provide persistent contaminants in freshwaters and the oceans from anthropogenic sources worldwide. MP contamination in ecosystems has emerged as a global environmental issue. While increasing research focused on the ecological consequences of plastic pollution, health-related implications of plastic pollution have been somewhat overlooked. In this study, we evaluated the effects of polyethylene MP contamination on microbial, physical, and biochemical characteristics of the hydrothermal vent crab Xenograpsus testudinatus over a 7-day food exposure. Different concentrations (0%, 0.3%, 0.6%, and 1.0%) of polyethylene MPs were used for feed intake experiments. Oxford Nanopore Technologies’ full-length sequencing of the 16S rDNA gene was used to explore the changes of the microbial composition in vent crab tissues. At the phylum level, the content of Firmicutes significantly decreased in the digestive gland tissue. Furthermore, the predicted functions of genes in the microbial community were significantly influenced by MPs. In contrast, there were eight functions in gill and 11 functions in digestive gland tissues identified at low and high intake levels. The dominant functions of methylotrophy, dark thiosulfate oxidation, dark oxidation of sulfur compounds, aromatic hydrocarbon degradation, and aromatic compound degradation were significantly increased at high intake levels in the digestive gland. These findings indicate that MP ingestion causes not only a short-term decrease in energy intake for crustaceans but also a change in microbial communities and their functions. This study provided a first account on the toxicity of MPs in a hydrothermal vent crab to aid in the assessment of health risks provided by polyethylene MP to marine invertebrates.

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