Adverse effects polystyrene microplastics exert on zebrafish heart – Molecular to individual level

Dimitriadi, Anastasia; Papaefthimiou, Chrisovalantis; Genizegkini, Eleni; Sampsonidis, Ioannis; Kalogiannis, Stavros; Feidantsis, Konstantinos; Bobori, Dimitra; Kastrinaki, Georgia; Koumoundouros, George; Lambropoulou, Dimitra A.; Kyzas, George Z.; Bikiaris, Dimitrios N.

doi:10.1016/j.jhazmat.2021.125969

Cited by 65 publications

(26 citation statements)

References 126 publications

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“…PS-MPs are spherical with average diameter size about 8 µm and as was found in our previous work are completely amorphous [52]. During zebrafish feeding we are expecting these to be entered by food to their bodies and accumulated to several organs.…”

Section: Ps-mps Characterizationsupporting

confidence: 62%

“…The preparation of PS microparticles was described in our previous work using the water oil emulsion technique [52]. As found by scanning electron microscopy, spherical PS-MPs with and mean average diameter about 8 ± 3 µm were prepared.…”

Section: Synthesis and Characterization Of Polystyrene Microplastics (Ps-mps)mentioning

confidence: 99%

“…In brief, an amount of PS-MPs (5-12 µm in size) in water suspension was incorporated into commercial powder fish food (different for each species as given above), to obtain the target concentrations (see below). Subsequently the mixture was well homogenized and dried in oven for about 2 h at 50 • C. Food for control groups was prepared following the same procedure as in previous study [52], omitting the addition of MPs. The heating of food could degrade food nutrient composition and possibly affect fish sensitivity to contaminants.…”

Section: Food Preparationmentioning

confidence: 99%

“…After gill and liver cells were treated with collagenase, DNA lysis and electrophoresis under neutral conditions, and DNA staining with acridine orange [71], the presence of comets was examined and counted under fluorescent microscope (Olympus CKX41) following the criteria of Ritter and Knebel [72]. Detailed description of the procedure of DNA damage is referred by Dimitriadi et al [52]. In brief, six slides per pool (zebrafish) and six slides per individual (perch) were measured, in order to represent technical replicates.…”

Section: Molecular and Biochemical Analysesmentioning

confidence: 99%

“…Several biomarkers have so far been suggested and examined in laboratory experimental exposure (via water or food) on fish thus offering a first set of implements that can be used to quantify the consequences that chemicals exert [46][47][48]. Among them oxidative stress biomarkers (as usually directly measured through free radical production, protein oxidation increase, lipid peroxidation), genotoxicity (DNA damage increase) and metabolic biomarkers (definition of differentiations in metabolic pathways) are considered as the most commonly used in such studies [49][50][51][52].…”

Section: Introductionmentioning

confidence: 99%

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Toxicity and Functional Tissue Responses of Two Freshwater Fish after Exposure to Polystyrene Microplastics

Kaloyianni

Bobori

Xanthopoulou

et al. 2021

Toxics

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Microplastics (MPs)’ ingestion has been demonstrated in several aquatic organisms. This process may facilitate the hydrophobic waterborne pollutants or chemical additives transfer to biota. In the present study the suitability of a battery of biomarkers on oxidative stress, physiology, tissue function and metabolic profile was investigated for the early detection of adverse effects of 21-day exposure to polystyrene microplastics (PS-MPs, sized 5–12 μm) in the liver and gills of zebrafish Danio rerio and perch, Perca fluviatilis, both of which are freshwater fish species. An optical volume map representation of the zebrafish gill by Raman spectroscopy depicted 5 μm diameter PS-MP dispersed in the gill tissue. Concentrations of PS-MPs close to the EC50 of each fish affected fish physiology in all tissues studied. Increased levels of biomarkers of oxidative damage in exposed fish in relation to controls were observed, as well as activation of apoptosis and autophagy processes. Malondialdehyde (MDA), protein carbonyls and DNA damage responses differed with regard to the sensitivity of each tissue of each fish. In the toxicity cascade gills seemed to be more liable to respond to PS-MPs than liver for the majority of the parameters measured. DNA damage was the most susceptible biomarker exhibiting greater response in the liver of both species. The interaction between MPs and cellular components provoked metabolic alterations in the tissues studied, affecting mainly amino acids, nitrogen and energy metabolism. Toxicity was species and tissue specific, with specific biomarkers responding differently in gills and in liver. The fish species that seemed to be more susceptible to MPs at the conditions studied, was P. fluviatilis compared to D. rerio. The current findings add to a holistic approach for the identification of small sized PS-MPs’ biological effects in fish, thus aiming to provide evidence regarding PS-MPs’ environmental impact on wild fish populations and food safety and adequacy.

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Section: Ps-mps Characterizationsupporting

confidence: 62%

Section: Synthesis and Characterization Of Polystyrene Microplastics (Ps-mps)mentioning

confidence: 99%

Section: Food Preparationmentioning

confidence: 99%

Section: Molecular and Biochemical Analysesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Toxicity and Functional Tissue Responses of Two Freshwater Fish after Exposure to Polystyrene Microplastics

Kaloyianni

Bobori

Xanthopoulou

et al. 2021

Toxics

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Synthesis and properties of biodegradable aliphatic–aromatic polyesters derived from 4‐hydroxybenzaldehyde

Wang

Cheng

Djouonkep

et al. 2023

J of Applied Polymer Sci

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A novel biphenyl monomer, diethyl 6,6′‐dimethoxy‐[1,1′‐bipheny]‐3,3′‐dicarboxylate (DDBD), was synthesized from 4‐hydroxybenzaldehyde, a phenolic compound derived from lignin. A series of polyesters were prepared by polycondensation of the biphenyl monomer with hydroquinone bis(2‐hydroxyethyl) ether and aliphatic diacids via a two‐step melt polycondensation method. It was shown that the properties of the polyesters can be tuned by varying the length and the structure of the spacer in the aliphatic diacid monomers. Structure–property relations for these materials were investigated by Fourier‐transform infrared spectroscopy, nuclear magnetic resonance spectroscopy, gel permeation chromatography (GPC), differential scanning calorimetry, thermogravimetric analysis, and dynamic mechanical analysis. GPC analysis showed that the weight‐average molecular weight (Mw) of the samples varied from 4.2–5.0 × 104 g/mol, while thermal analysis yielded Tg values of 51–120°C, and 5% decomposition temperatures (Td,5%) of 327–389°C. All the samples exhibited a high yield strength (56–87 MPa). After 30 weeks of degradation in soil, the copolyesters had mass losses of up to 4.4%, and an investigation of the ecotoxicity of the copolyesters with Eisenia fetida yielded a survival rate of over 80% after 14 days for the different copolyesters, suggesting low toxicity in the environment. Overall, these copolyesters have good properties and would be suitable to replace petroleum‐based commercial poly(ethylene terephthalate) packaging materials.

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Combined toxicity of polystyrene microplastics and sulfamethoxazole on zebrafish embryos

Gong

et al. 2021

Environ Sci Pollut Res

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Adverse effects polystyrene microplastics exert on zebrafish heart – Molecular to individual level

Cited by 65 publications

References 126 publications

Toxicity and Functional Tissue Responses of Two Freshwater Fish after Exposure to Polystyrene Microplastics

Toxicity and Functional Tissue Responses of Two Freshwater Fish after Exposure to Polystyrene Microplastics

Synthesis and properties of biodegradable aliphatic–aromatic polyesters derived from 4‐hydroxybenzaldehyde

Combined toxicity of polystyrene microplastics and sulfamethoxazole on zebrafish embryos

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