Adverse Effect of Air Exposure on the Stability of DNA Stored at Room Temperature

Colotte, Marthe; Coudy, Delphine; Tuffet, Sophie; Bonnet, Jacques

doi:10.1089/bio.2010.0028

Cited by 36 publications

(39 citation statements)

References 19 publications

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“…Degradation of DNA increases with dilution, higher storage temperature, multiple suspensions, and repeated freeze-thaw cycles (99,116,117 ). Special technologies for storing DNA at room temperature have been developed, enabling easier shipment of these extracts (111, 116 -118 ).…”

Section: Extracted Dnamentioning

confidence: 99%

Preanalytical Variables Affecting the Integrity of Human Biospecimens in Biobanking

Ellervik

Vaught²

2015

Clinical Chemistry

137

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BACKGROUND Most errors in a clinical chemistry laboratory are due to preanalytical errors. Preanalytical variability of biospecimens can have significant effects on downstream analyses, and controlling such variables is therefore fundamental for the future use of biospecimens in personalized medicine for diagnostic or prognostic purposes. CONTENT The focus of this review is to examine the preanalytical variables that affect human biospecimen integrity in biobanking, with a special focus on blood, saliva, and urine. Cost efficiency is discussed in relation to these issues. SUMMARY The quality of a study will depend on the integrity of the biospecimens. Preanalytical preparations should be planned with consideration of the effect on downstream analyses. Currently such preanalytical variables are not routinely documented in the biospecimen research literature. Future studies using biobanked biospecimens should describe in detail the preanalytical handling of biospecimens and analyze and interpret the results with regard to the effects of these variables.

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Section: Extracted Dnamentioning

confidence: 99%

Preanalytical Variables Affecting the Integrity of Human Biospecimens in Biobanking

Ellervik

Vaught²

2015

Clinical Chemistry

137

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“…The minicapsules were then laserwelded under anhydrous argon according to the process developed by Imagene company. 24 When needed, capsules were opened and RNA was rehydrated by adding 20 or 30 ml of water, followed by 10-min incubation on ice.…”

Section: Rna Encapsulationmentioning

confidence: 99%

“…For studying the effect of moist air exposure, a set of minicapsules were opened and placed in an atmosphere of approximately 50% relative humidity inside closed glass bottles as previously described in. 23,24 At each time point, the capsules were quickly removed from the oven and stored at À20 1C until analysis. The t 0 time was set at 10 min to allow the establishment of temperature and hydration equilibriums.…”

Section: Accelerated Degradation Studiesmentioning

confidence: 99%

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An efficient method for long-term room temperature storage of RNA

Fabre

Colotte²,

Luis³

et al. 2013

Eur J Hum Genet

Self Cite

107

110

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RNA is a tool used in many fields, from molecular and cellular biology to medicine and nanotechnology. For most of these uses, the integrity of RNA is required and must be maintained during storage. Even though freezing is currently the storage method of choice, the increasing number of samples to be stored and the costly use of a cold chain have highlighted the need for room temperature preservation methods. Here, we report a new room temperature technology that consists in drying RNA samples in the presence of a stabilizer in stainless steel minicapsules. These air-and water-tight capsules isolate RNA from the atmosphere and maintain an anhydrous and anoxic environment. Through the evaluation of RNA integrity over time at room temperature or 90 1C, we identified atmospheric humidity as a major deleterious factor. The degradation rate dependence in temperature fitted an Arrhenius model, with an activation energy of 28.5 kcal/mol and an extrapolated room temperature degradation rate of 3.2 10 À13 /nt/s (95% confidence interval: 2.3-4.2/nt/s). In these conditions, it is expected that an RNA molecule will be subjected to 0.7-1.3 cut every 1000 nucleotides per century. In addition, we showed that stored RNA is compatible for further analyses, such as reverse transcription-quantitative PCR. No significant change in the C q values was observed over a simulated period of several decades. At last, our data are consistent with a sequence-independent degradation rate of RNA in the solid state.

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“…Despite the growing body of research on nonviral vector dehydration, to date, lyophilization studies have mostly focused on the stability of vectors (e.g., naked DNA [91,[110][111][112][113][114], lipoplexes [83,86,[115][116][117], polyplexes [85,88,99,109,118,119], and lipopolyplexes [95,120]) during acute freeze-drying stress. Considering the complexity involved in the stabilization of individual vectors (e.g., naked DNA, lipid) as dehydrated formulations, this chapter addresses these challenges separately.…”

Section: Introductionmentioning

confidence: 99%

Stabilization of Plasmid DNA and Lipid-Based Therapeutics as Dehydrated Formulations

Molina

Payton

Anchordoquy

2015

Lyophilized Biologics and Vaccines

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Adverse Effect of Air Exposure on the Stability of DNA Stored at Room Temperature

Cited by 36 publications

References 19 publications

Preanalytical Variables Affecting the Integrity of Human Biospecimens in Biobanking

Preanalytical Variables Affecting the Integrity of Human Biospecimens in Biobanking

An efficient method for long-term room temperature storage of RNA

Stabilization of Plasmid DNA and Lipid-Based Therapeutics as Dehydrated Formulations

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