Adventitious shoot regeneration in peach [Prunus persica (L.) Batsch]

Gentile, A.; Monticelli, S.; Damiano, C.

doi:10.1007/s00299-002-0451-2

Cited by 86 publications

(64 citation statements)

References 21 publications

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“…Multiple shoots of Himalayan oaks were induced from intact embryos and cotyledonary nodes (Purohit et al 2002). Recently, a method for adventitious shoot regeneration from leaf explants of micropropagated peach shoots has been developed (Gentile et al 2002).…”

Section: Micropropagation Of Tree Without Intervening Callus Phasementioning

confidence: 99%

Progress in tissue culture, genetic transformation and applications of biotechnology to trees: an overview

Giri

Shyamkumar

Anjaneyulu

2003

Trees

168

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Trees are an integral part of human life, and a vital component of biodiversity. Forest trees in particular are renewable sources of food, fodder, fuel wood, timber and other valuable non-timber products. Due to the rapid growth of population and the human desire to progress, there has been a tremendous reduction in forest cover from the earth's surface. To maintain and sustain forest vegetation, conventional approaches have been exploited in the past for propagation and improvement. However, such efforts are confronted with several inherent bottlenecks. Biotechnological interventions for in vitro regeneration, mass micropropagation and gene transfer methods in forest tree species have been practised with success, especially in the last decade. Against the background of the limitations of long juvenile phases and life span, development of plant regeneration protocols and genetic engineering of tree species are gaining importance. Genetic engineering assumes additional significance, because of the possibility of introducing a desired gene in a single step for precision breeding of forest trees. There are no comprehensive and detailed reviews available combining research developments with major emphases on tissue culture and basic genetic transformation in tree species. The present communication attempts to overview the progress in tissue culture, genetic transformation and biotechnological applications in the last decade and future implications.

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Section: Micropropagation Of Tree Without Intervening Callus Phasementioning

confidence: 99%

Progress in tissue culture, genetic transformation and applications of biotechnology to trees: an overview

Giri

Shyamkumar

Anjaneyulu

2003

Trees

168

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“…Due to quarantine restrictions, it has become increasingly difficult to bring new Prunus germplasm into the United States for breeding; hence, genetic engineering may be a useful option for introducing genes for resistance to microbial and insect pests into Prunus. In vitro culture systems for Prunus species have been developed using seed explants (Mante et al 1989;Pooler and Scorza 1995;Reidiboym-Talleux et al 1999;Hokanson and Pooler 2000) and vegetative tissues (Druart 1990;Machado et al 1995;GutierrezPesce et al 1998;Mandegaran et al 1999;Gentile et al 2002). The objective of the investigation reported here was to evaluate factors that affect somatic embryogenesis from root explants in the flowering cherry cultivar Prunus incisa Thunb.…”

Section: Introductionmentioning

confidence: 95%

Factors affecting somatic embryogenesis in Prunus incisa cv. February Pink

Cheong

Pooler

2004

Plant Cell Reports

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Factors affecting somatic embryogenesis from root explants of Prunus incisa Thunb. cv. February Pink were investigated. Using a medium containing Murashige and Skoog salts and vitamins supplemented with 10 microM 2,4-dichlorophenoxyacetic (2,4-D), we evaluated the effects of light, growth regulators, amino acids, carbohydrate source, and root induction medium. Explants cultured under light or dark conditions both resulted in the formation of embryos. Embryogenesis was inhibited by the addition of 6-benzyladenine, thidiazuron, or gibberellic acid to the medium. Amino acids were not effective in promoting embryogenesis, with high levels of amino acids actually inhibiting it. Sucrose and glucose effectively induced embryogenesis, while sorbitol and mannitol completely inhibited it. Sucrose and glucose also promoted secondary embryogenesis. Embryos that formed in medium containing 4% or 5% sucrose were abnormally shaped and did not fully develop, while those that formed in medium with sucrose concentrations of 2% or 3% were much more vigorous. Root explants that were induced on medium containing 1.0 micro M indole-3-butyric acid (IBA) produced more somatic embryos than explants induced on medium without IBA. Approximately 50% of the roots induced on medium containing 1.0 microM IBA produced somatic embryos on medium containing 10 microM 2,4-D and 3% sucrose.

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“…Fruit trees are among the most recalcitrant in producing adventitious shoots, but in recent years some successful attempts at regeneration and transformation of different Prunus species have been reported (Druart 1980;Ochatt 1990;Hammatt 1993;da Câmara Machado et al 1995;Declerck and Korban 1996;Miguel et al 1996;Gutirrez-Pesce et al 1998;Hammatt and Grant 1998;Miguel and Oliveira 1999;Ainsley et al 2000;Gentile et al 2002;Tang et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Ethylene inhibitors and low kanamycin concentrations improve adventitious regeneration from apricot leaves

Burgos

Alburquerque

2003

Plant Cell Reports

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An improved method for adventitious regeneration from apricot leaves is described. The use of the ethylene inhibitors silver thiosulphate (30-60 micro M) or aminoethoxyvinylglycine (0.5 micro M) increased regeneration percentages in Helena and Canino apricot cultivars and also the consistency of results from different experiments. Use of "Pure Agar" also improved regeneration from Helena leaves as compared with agargel or agarose. Regeneration rates for Canino were dependent on the medium in which the shoots were micropropagated. When different antibiotics were tested for their influence on regeneration, the combination of cefotaxime (0.13 m M) plus vancomycin (0.63 m M), which efficiently controls Agrobacterium growth, also increased regeneration percentages in Helena two-fold but did not affect regeneration in Canino. Kanamycin, an antibiotic widely used for selection of nptII transformed cells, promoted more rapid regeneration and higher regeneration rates from Helena leaves when added at low concentrations (8.6 and 17.1 micro M). With this improved procedure, regeneration from apricot leaves has been increased more than 200% as compared with rates reported previously.

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Adventitious shoot regeneration in peach [Prunus persica (L.) Batsch]

Cited by 86 publications

References 21 publications

Progress in tissue culture, genetic transformation and applications of biotechnology to trees: an overview

Progress in tissue culture, genetic transformation and applications of biotechnology to trees: an overview

Factors affecting somatic embryogenesis in Prunus incisa cv. February Pink

Ethylene inhibitors and low kanamycin concentrations improve adventitious regeneration from apricot leaves

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