adventitious shoot regeneration from petiole explants of Heracleum candicans wall

Sharma, Rajesh; Wakhlu, A. K.

doi:10.1007/s11627-001-0131-x

Cited by 14 publications

(10 citation statements)

References 13 publications

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“…The regeneration efficiency and number of shoot buds were higher in horizontally placed explants as compared to vertically placed explants irrespective of the source of explants. Similar findings reported in Heracleum candicans (Sharma and Wakhlu 2001) and Eryngium foetidum (Arockiasamy et al 2002) may be due to little contact of the explants to the medium in a vertical position as compared to a horizontal position. In vitro explants had a higher rate of regeneration and number of shoot buds as compared to in vivo explants in both the horizontal and vertical position.…”

Section: Discussionsupporting

confidence: 77%

In vitro plant regeneration of non-toxic Jatropha curcas L.: Direct shoot organogenesis from cotyledonary petiole explants

Kumar

Anand

Reddy

2010

J. Crop Sci. Biotechnol.

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Jatropha curcas, the energy plant has attained great attention in recent years because of its biodiesel production potential; however, oil and deoiled cakes are toxic. A non-toxic variety of J. curcas is reported from Mexico. A simple and efficient protocol has been developed for plant regeneration using cotyledonary petiole explants of non-toxic variety of J. curcas. The percentage of induction of shoot buds (59.11%), and the number of shoot buds (5.01) per explant was achieved on Murashige and Skoog's (MS) medium supplemented with 2.27 µM thidiazuron (TDZ). These induced shoot buds multiplied when subcultured on MS medium supplemented with 10 µM kinetin (Kn), 4.5 µM 6-benzyl aminopurine (BAP), and 5.5 µM -naphthaleneacetic acid (NAA) for 4 weeks and subsequent elongation achieved on MS medium supplemented with 2.25 µM BAP and 8.5 µM indole-3-acetic acid (IAA). Shoots more than 2 cm long were harvested and cultured on MS medium containing different concentrations and combinations of IBA, IAA, NAA, and 0.25 mg L -1 activated charcoal, and 19.91% rooting was achieved in 15 µM IBA, 5.7 µM IAA, and 16.5 µM NAA after 4 weeks with more than 90% survival rate.

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Section: Discussionsupporting

confidence: 77%

In vitro plant regeneration of non-toxic Jatropha curcas L.: Direct shoot organogenesis from cotyledonary petiole explants

Kumar

Anand

Reddy

2010

J. Crop Sci. Biotechnol.

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“…Only micropropagation through axillary bud sprouting (Sharma and Chandel 1992) has been reported on this plant. It has been shown that shoot organogenesis via an intermediate callus phase can be used as an effective method for multiplication of other medicinal plants (Sarasan et al 1994, Ahroni et al 1997, Castillo and Jordan 1997, Sharma and Wakhlu 2001, Sin and Teng 2002. The present communication is the first report on an efficient in vitro propagation and multiplication of Tylophora indica via stem callus culture.…”

Section: ⎯⎯⎯⎯mentioning

confidence: 99%

An efficient in vitro method for mass propagation of Tylophora indica

Faisal¹,

Anis²

2005

Biol. plant.

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A protocol of high frequency shoot organogenesis and plant establishment from stem derived callus has been developed for Tylophora indica (Burm. f.) Merrill. -an endangered medicinal plant. Callus was developed on Murashige and Skoog (MS) medium supplemented with 10 µM 2,4,5-trichlorophenoxy acetic acid (2,4,5-T). Multiple shoot induction was achieved from the surface of the callus after transferring onto shoot induction medium. The highest rate (80 %) of shoot multiplication was achieved on MS medium containing 5.0 µM kinetin. The developed shoots rooted best on halfstrength MS medium supplemented with 0.5 µM indole-3-butyric acid (IBA). The in vitro raised plantlets with well developed shoot and roots were acclimatized successfully and grown in greenhouse.Additional key words: callus culture, growth regulators, medicinal plant, shoot multiplication, 2,4,5-trichlorophenoxy acetic acid. ⎯⎯⎯⎯Tylophora indica (Asclepiadaceae) is a perennial climbing plant native to the plains, forest and hills of southern and eastern India. The pharmacological importance of this plant is mainly due to the presence of alkaloid tylophorine and tylophorenine. Besides, root contains a potential anti-tumor alkaloid tylophorinidine (Mulchandani et al. 1971).The over-exploitation of Tylophora indica from the nature and inadequate efforts for its cultivation resulted in marked decline in the population of this species. Therefore, it is necessary to develop methods for in vitro propagation and conservation of this plant. Only micropropagation through axillary bud sprouting (Sharma and Chandel 1992) has been reported on this plant. It has been shown that shoot organogenesis via an intermediate callus phase can be used as an effective method for multiplication of other medicinal plants (Sarasan et al. 1994, Ahroni et al. 1997, Castillo and Jordan 1997, Sharma and Wakhlu 2001, Sin and Teng 2002. The present communication is the first report on an efficient in vitro propagation and multiplication of Tylophora indica via stem callus culture.The young shoots of Tylophora indica collected from plants grown at the Botanical Garden, Aligarh Muslim University, were washed under running tap water for at least 30 min, followed by soaking in 5 % (v/v) Teepol for 5 min. After a thorough washing in sterile distilled water, the source tissues were surface sterilized with 0.1 % (m/v) HgCl 2 for 3 min. Following repeated washes with sterile distilled water, the stem segments were cut into appropriate size (0.5 -1 cm) and transferred onto culture medium.The nutrient medium consisted Murashige and Skoog (1962; MS) salts and vitamins and 3 % (m/v) sucrose was used in all experiments. The pH of medium was adjusted to 5.8 prior to the addition of 0.8 % (m/v) agar and autoclaving at 121 °C and 1.06 kg cm -2 pressure for 20 min. The cultures were incubated at 25 ± 2 °C under 16-h photoperiod (irradiance of 50 µmol m -2 s -1 ) and relative humidity of 60 %.For callus induction from stem segments, four concentrations (0.5 -10 µM) of each 2,4-dichlorophenoxy acetic ac...

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“…The substrates usually used for acclimatization with other members of that family (Sharma and Wakhlu 2001, Nath and Buragohain 2003, Tavares et al 2010a, Coste et al 2012, Hendrawati et al 2012, Jaheduzzaman et al 2012 were far different from the substrate which promoted acclimatization of our plant species. H. pastinacifolia did not acclimatize at all if the substrates were rich in organic matter (data not shown).…”

Section: Acclimatization and Transfer To In Vivo Conditionsmentioning

confidence: 95%

“…Root development with an intermediary callus indicated that at that roots and shoots were not well connected with vascular tissue and that plants are insuffi ciently supplied with nutrients (George 1993). Successful rooting in other members of the Apiaceae family was also achieved with 1-5 μM IBA (Tavares et al 2010a, Das et al 2008, Jana and Shekhawat 2011, Coste et al 2012, Jaheduzzaman et al 2012, and with higher concentrations of IBA (Hossain et al 2000, Sharma and Wakhlu 2001, Banerjee et al 1999, Tavares et al 2009-2010, with NAA or IAA (Karuppusamy et al 2007, Jaheduzzaman et al 2012) and only rarely without growth regulators (Makunga et al 2003).…”

Section: Root Initiationmentioning

confidence: 99%

Micropropagation of the Narrow Endemic Hladnikia pastinacifolia (Apiaceae)

Ambrožič-Dolinšek¹,

Ciringer²,

Kaligarič³

2016

Acta Botanica Croatica

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-The monotypic Hladnikia pastinacifolia Rchb. is a narrow endemic species, with an extremely small distribution area in Slovenia, prone to any kind of threat that could lead to species extinction. Tissue culture techniques are proposed as a conservation measure for rapid propagation and ex-situ conservation. Tissue culture was initiated from seeds and juvenile plants obtained from natural sites on a solid Murashige and Skoog (MS) medium, with and without growth regulators. We tested various combinations and concentrations of growth regulators, and the best proliferation of axillary shoots, on average 14, was obtained on MS medium with 5 μM BAP and 3 μM IBA and 3% sucrose. Rooting was achieved after transferral of the shoots to an MS medium with 2 μM IBA and 3% sucrose. The rooted plants were acclimatized on a mixture of limestone sand, potting soil and vermiculite in a ratio of 10:2:2, with pH in the range of 7.5-8.0. In vitro propagation methods provide an important opportunity for the propagation and preservation of H. pastinacifolia by rapidly increasing the number of plants, without disturbing the wild population.

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adventitious shoot regeneration from petiole explants of Heracleum candicans wall

Cited by 14 publications

References 13 publications

In vitro plant regeneration of non-toxic Jatropha curcas L.: Direct shoot organogenesis from cotyledonary petiole explants

In vitro plant regeneration of non-toxic Jatropha curcas L.: Direct shoot organogenesis from cotyledonary petiole explants

An efficient in vitro method for mass propagation of Tylophora indica

Micropropagation of the Narrow Endemic Hladnikia pastinacifolia (Apiaceae)

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