Adventitious shoot formation in cultured leaf explants of quince and pear is accompanied by different patterns of ethylene and polyamine production, and responses to aminoethoxyvinylglycine

Marino, Grazia; Franchin, Cinzia; Marcolini, Graziella; Biondi, Stefania

doi:10.1080/14620316.2008.11512378

Cited by 10 publications

(15 citation statements)

References 28 publications

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“…Donor shoot cultures of ‘BA29’ quince ( C. oblonga L.) and ‘Conference’ pear ( P. communis L.) were respectively maintained on previously experimented quince (QPM) and pear (PPM) proliferation media through monthly subcultures. The former was based on a modified Murashige and Skoog composition and the latter on a modified Quoirin and Lepoivre medium.…”

Section: Methodsmentioning

confidence: 99%

“…Three transverse incisions were made on the leaves (lacking petiole), and they were placed (5 leaves per dish) with the abaxial side down in 9 cm diameter, vented polystyrene Petri dishes (Sarstedt S.R.L., Verona, Italy), each containing 20 mL of QIM or PIM medium. They were sealed with Parafilm (preventing gas exchanges) for pear and with PVC strips for quince, following previous results on factors positively affecting regeneration. The explants were then transferred to QDM or PDM in Petri dishes that were sealed with PVC film for better shoot growth .…”

Section: Methodsmentioning

confidence: 99%

“…Organogenesis is critically affected by medium composition and physical factors, although it also strongly depends on the morphogenic potential or competence of plant tissue cultures, which may vary with genotype, tissue age, explant choice, and polarity. In indirect organogenesis, it is unclear whether meristemoids that are formed within callus are always already determined to give origin to one specific organ or whether meristemoid and organ formation are separately and sequentially decided as a consequence of culture conditions. , Previous research showed that different interacting factors can affect shoot regeneration from leaf explants in quince − and pear, , although the latter generally displayed a low regeneration capacity. , Therefore, it seems of great significance to explore alternatives to traditional treatments to improve plant regeneration from somatic tissues.…”

Section: Introductionmentioning

confidence: 99%

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Effect of a Peat Humic Acid on Morphogenesis in Leaf Explants of Pyrus communis and Cydonia oblonga. Metabolomic Analysis at an Early Stage of Regeneration

Marino

Righi

Simoni

et al. 2013

J. Agric. Food Chem.

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Plant regeneration is a critical step in most in vitro breeding techniques. This paper studies the effects of a low-molecular-weight humic acid (HA) on morphogenesis from pear and quince leaf explants. Variable HA amounts [0 (control), 1, 5, 10, and 20 mg C L(-1)] were added to the regeneration media. A dose-response effect was observed in pear for root and shoot production; it was improved at HA 1 mg C L(-1) and considerably reduced at the highest amounts. HA was, instead, ineffective in quince. The (1)H HR-MAS NMR analyses of calli in the induction phase showed more evident metabolite (asparagine, alanine, and γ-aminobutyric acid) signals in quince than in pear. The assignment of overlapped signals in both genotypes was supported by the 2D NMR analyses. Spectroscopic characterization suggested also an enhancement of asparagine contents in morphogenic calli of pear with respect to the control and higher HA amount treatments.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of a Peat Humic Acid on Morphogenesis in Leaf Explants of Pyrus communis and Cydonia oblonga. Metabolomic Analysis at an Early Stage of Regeneration

Marino

Righi

Simoni

et al. 2013

J. Agric. Food Chem.

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“…They are interrelated through both their biosynthetic routes as well as their physiological roles (Alvarez et al 2003). Several studies have analyzed the relationship between different morphogenic capacities within the same (Pua 1999;El Meskaoui and Tremblay 2001;Shoeb et al 2001;Li and Burritt 2003;Zapata et al 2004;Hu et al 2006) or different (Marino et al 2008) species using various indicators other than polyamine and ethylene levels.…”

Section: Introductionmentioning

confidence: 99%

Ethylene and polyamine production patterns during in vitro shoot organogenesis of two passion fruit species as affected by polyamines and their inhibitor

Dias

Santa–Catarina

Ribeiro

et al. 2009

Plant Cell Tiss Organ Cult

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Levels of ethylene and polyamines (PAs) were measured during organogenesis of hypocotyl explants of two species of passion fruit (Passiflora cincinnata Masters and Passiflora edulis Sims f. flavicarpa Degener 'FB-100') to better understand the relationships of these regulators and their influence on cell differentiation and morphogenesis. Moreover, histological investigation of shoot ontogenesis was conducted to characterize the different events involved in cell redifferentiation and regulation of PA and ethylene levels. A delay was observed in morphogenic responses of P. edulis f. flavicarpa as compared to P. cincinnata, and these changes coincided with production of elevated levels of polyamine and ethylene levels. During differentiation, cells showed high rates of expansion and elongation, and high ethylene levels were associated with high PA levels, suggesting that the two biosynthesis pathways were highly regulated. Moreover, their interaction might be an important factor for determining cell differentiation. The addition of PAs to the culture medium did not promote organogenesis; however, the incorporation of the PA inhibitor methylglyoxal bisguanylhydrazone in the culture medium reduced shoot bud differentiation, suggesting the need to maintaining a minimum level of PAs for morphogenic events to take place.

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“…Ethylene greatly influences the regeneration capacity of plant material, either promoting or inhibiting morphogenetic processes in several species, such as Nicotiana tabacum (Biondi et al 1998), Malus domestica (Ma et al 1998), Phaseolus vulgaris (Carvalho et al 2000), Passiflora edulis f. flavicarpa (Reis et al 2003), Carica papaya (Magdalita et al 1997), Bixa orellana (Paiva Neto et al 2009) Actinidia deliciosa (Arigita et al 2003), Arachis hypogaea (Ozudogru et al 2005), Capsicum chinense (Santana-Buzzy et al 2006), and Cydonia oblonga (Marino et al 2008). Results suggest that there is a relationship between sensitivity of seedlings to ethylene and the climacteric characteristics of the fruit (Jona et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Ethylene and polyamine interactions in morphogenesis of Passiflora cincinnata: effects of ethylene biosynthesis and action modulators, as well as ethylene scavengers

et al. 2010

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Ethylene is a plant hormone that is of fundamental importance to in vitro morphogenesis, but in many species, it has not been thoroughly studied. Its relationship with polyamines has been studied mainly because the two classes of hormones share a common biosynthetic precursor, S-adenosylmethionine (SAM). In order to clarify whether competition between polyamines and ethylene influences in vitro morphogenetic responses of Passiflora cincinnata Mast., a climacteric species, different compounds were used that act on ethylene biosynthesis and action, or as ethylene scavengers. Treatment with the ethylene inhibitor, aminoethoxyvinylglycine (AVG) caused a greater regeneration frequency in P. cincinnata, whereas treatment with the ethylene precursor, 1-aminocyclopropane-1-carboxylic-acid (ACC) lessened regeneration frequencies. The data suggested that levels of polyamines and ethylene are not correlated with morphogenic responses in P. cincinnata. It was ascertained that neither the absolute ethylene and polyamine levels, nor competition between the compounds, correlated to the obtained morphogenic responses. However, sensitivity to, and signaling by, ethylene appears to play an important role in differentiation. This study reinforces previous reports regarding the requirement of critical concentrations and temporal regulation of ethylene levels for morphogenic responses. Temporal regulation also appeared to be a key factor in competition between the two biosynthetic pathways, without having any effects on morphogenesis. Further studies investigating the silencing or overexpression of genes related to ethylene perception, under the influence of polyamines in cell differentiation are extremely important for the complete understanding of this process.

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Adventitious shoot formation in cultured leaf explants of quince and pear is accompanied by different patterns of ethylene and polyamine production, and responses to aminoethoxyvinylglycine

Cited by 10 publications

References 28 publications

Effect of a Peat Humic Acid on Morphogenesis in Leaf Explants of Pyrus communis and Cydonia oblonga. Metabolomic Analysis at an Early Stage of Regeneration

Effect of a Peat Humic Acid on Morphogenesis in Leaf Explants of Pyrus communis and Cydonia oblonga. Metabolomic Analysis at an Early Stage of Regeneration

Ethylene and polyamine production patterns during in vitro shoot organogenesis of two passion fruit species as affected by polyamines and their inhibitor

Ethylene and polyamine interactions in morphogenesis of Passiflora cincinnata: effects of ethylene biosynthesis and action modulators, as well as ethylene scavengers

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