Adventitious regeneration of blackberry, blueberry, and kiwiberry and assessment of genetic stability by ISSR markers

Hunková, Júlia; Kleman, Juraj; Gažo, J.; Gajdošová, Alena

doi:10.1007/s11756-022-01211-7

Cited by 3 publications

(1 citation statement)

References 37 publications

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“…The adventitious shoot regeneration technique has been successfully employed for mass propagation of berry crops using semisolid ( Qiu et al, 2018 ; Schuchovski et al., 2020 ) and liquid media in bioreactors ( Debnath, 2014 ; Arigundam et al., 2020 ). While working with blackberry ( Rubus fruticosus ), blueberry ( V. corymbosum ), and kiwiberry ( Actinidia argute ), Hunková et al. (2022) regenerated shoots from petioles and internodal segments; Verma et al.…”

Section: In Vitro Propagationmentioning

confidence: 99%

Phenotypic variation and epigenetic insight into tissue culture berry crops

Debnath

Ghosh

2022

Front. Plant Sci.

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Berry crops, a nutrient powerhouse for antioxidant properties, have long been enjoyed as a health-promoting delicious food. Significant progress has been achieved for the propagation of berry crops using tissue culture techniques. Although bioreactor micropropagation has been developed as a cost-effective propagation technology for berry crops, genetic stability can be a problem for commercial micropropagation that can be monitored at morphological, biochemical, and molecular levels. Somaclonal variations, both genetic and epigenetic, in tissue culture regenerants are influenced by different factors, such as donor genotype, explant type and origin, chimeral tissues, culture media type, concentration and combination of plant growth regulators, and culture conditions and period. Tissue culture regenerants in berry crops show increased vegetative growth, rhizome production, and berry yield, containing higher antioxidant activity in fruits and leaves that might be due to epigenetic variation. The present review provides an in-depth study on various aspects of phenotypic variation in micropropagated berry plants and the epigenetic effects on these variations along with the role of DNA methylation, to fill the existing gap in literature.

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Section: In Vitro Propagationmentioning

confidence: 99%

Phenotypic variation and epigenetic insight into tissue culture berry crops

Debnath

Ghosh

2022

Front. Plant Sci.

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Optimized protocol for high-frequency papaya propagation: morpho-stereomicroscopic analysis and genetic fidelity assessment

Vishal,

Sidhu,

Gaikwad

et al. 2024

Plant Cell Tiss Organ Cult

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Cryopreservation of highbush blueberry, strawberry, and saskatoon using V and D cryo-plate methods and monitoring of multiplication ability of regenerated shoots

Vujović,

Anđelić,

Marković

et al. 2024

In Vitro Cell.Dev.Biol.-Plant

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The purpose of this work was to apply V and D cryo-plate methods for cryopreservation of Vaccinium corymbosum ʻToroʼ, Fragaria × ananassa ʻCleryʼ, and Amelanchier alnifolia (Nutt.) M. Roem. and to monitor the multiplication capacity of shoots regenerated from cryopreserved explants. Shoot tips pre-cultured for 1 d at 23°C in the dark on medium containing 0.3 M sucrose were used as explants. Loading was performed in a solution containing 2 M glycerol and 0.8 M sucrose (30 min at room temperature). In the V cryo-plate, dehydration was carried out at room temperature (20 to 50 min) using the following plant vitrification solutions: original PVS2, 90% PVS2 solution, and PVS3. Regarding the D cryo-plate, dehydration was performed in closed glass containers over silica gel for 2, 2.5, or 3 h. In both protocols, rewarming was carried out in a 1.0 M sucrose solution (15 min at 25°C). Regenerated shoots were multiplied and multiplication parameters were monitored after the second subculture. Using the V cryo-plate method, the highest regrowth in highbush blueberry was obtained following 50-min treatment with all three VSs (61.7 to 80.9%). The D cryo-plate method was even more suitable with maximum regrowth of 89.4% achieved after 2.5 h of desiccation. For strawberry, 62.5% was the highest regrowth recorded using PVS3-based V cryo-plate method while 83.3% of regrowth was observed using D cryo-plate protocol. Regrowth of saskatoon reached a maximum of 50% after 50-min treatment with PVS3 while it did not exceed 40% in other treatments. By the second subculture, shoots regenerated from cryopreserved explants regained and even exceeded the multiplication capacity of shoots regenerated from non-cryopreserved explants. This study is the first to present the successful application of the V cryo-plate method in highbush blueberry, as well as the utilization of both V and D cryo-plate methods in saskatoon.

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Adventitious regeneration of blackberry, blueberry, and kiwiberry and assessment of genetic stability by ISSR markers

Cited by 3 publications

References 37 publications

Phenotypic variation and epigenetic insight into tissue culture berry crops

Phenotypic variation and epigenetic insight into tissue culture berry crops

Optimized protocol for high-frequency papaya propagation: morpho-stereomicroscopic analysis and genetic fidelity assessment

Cryopreservation of highbush blueberry, strawberry, and saskatoon using V and D cryo-plate methods and monitoring of multiplication ability of regenerated shoots

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