Adventitious embryo formation derived from zygotic embryos in Cycas revoluta

Motohashi, Tsuyoshi; Toda, M.; Kondo, Katsuhiko

doi:10.5511/plantbiotechnology.25.589

Cited by 2 publications

(4 citation statements)

References 10 publications

(5 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Osborne (1990) reported callus formation on mature ZEs of Encephalartos villosus cultured on SH medium supplemented with 3.0 mg l -1 BA and 0.3 mg l -1 2,4-D. Unlike our study in which the same PGR combination resulted in callus formation, Motohashi et al (2008) induced adventitious shoots from mature C. revoluta ZEs on SH medium supplemented with 0.20 mg l -1 BA and 0.02 mg l -1 2,4-D. Creamy, friable callus (Fig. 1A) could not differentiate into any organ.…”

Section: Resultscontrasting

confidence: 69%

“…Highest proliferation (88.7±40.5 adventitious shoots ZE -1 ) was in the presence of 0.20 mg l -1 BA and 0.02 mg l -1 2,4-D. Except for our study, the Motohashi et al (2008) paper is the only other study that acclimatized C. revoluta in vitro-derived plantlets. C. revoluta continues to be recalcitrant to in vitro propagation by conventional methods.…”

Section: Introductionmentioning

confidence: 64%

“…ZEs are a stable and useful explant for regeneration of difficult-to-regenerate species like cycads (Elhiti and Stasolla, 2011). Motohashi et al (2008) successfully induced multiple adventitious shoots from mature C. revoluta ZEs in SH medium supplemented with a combination of BA (0.20 to 2.00 mg l -1 ) and 2,4-D (0.00 to 0.20 mg l -1 ) 42 to 84 days after the beginning of culture. Highest proliferation (88.7±40.5 adventitious shoots ZE -1 ) was in the presence of 0.20 mg l -1 BA and 0.02 mg l -1 2,4-D.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Simplified Regeneration Protocol for Cycas revoluta Thunb. Mature Zygotic Embryos

Naderi

Mohaiseni

Silva³

et al. 2015

Not Sci Biol

View full text Add to dashboard Cite

Mature zygotic embryos of Cycas revoluta Thunb. were used as explants to investigate direct and indirect organogenesis. Explants were incubated on half-strength Murashige and Skoog (½ MS) basal medium supplemented with various plant growth regulators, singly or in combination (all at 0.5 mg l-1): 6-benzyladenine (BA), kinetin (Kin), 2,4-dichlorophenoxyacetic acid (2,4-D), Kin×2,4-D, BA×Kin and BA×2,4-D. Cultures were placed at a low light intensity (4 µmol m-2 s-1 PPFD). Adventitious shoot regeneration was observed in the presence of 0.5 mg l-1BA after 35 days. The highest number of direct and indirect shoots per zygotic embryo was 3.67 and 29.67, respectively. Roots were induced on indirect shoots by continuous culture on rooting medium (½ MS,‏ 0.1 mg l-1 1-naphthaleneacetic acid) and hardened successfully in perlite. Each rooted plantlet with pinnate leaves and a primary tap root was individually isolated and acclimatized 185 days after the beginning of culture, with a 10% success rate.

show abstract

Section: Resultscontrasting

confidence: 69%

Section: Introductionmentioning

confidence: 64%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Simplified Regeneration Protocol for Cycas revoluta Thunb. Mature Zygotic Embryos

Naderi

Mohaiseni

Silva³

et al. 2015

Not Sci Biol

View full text Add to dashboard Cite

show abstract

“…To increase the efficiency of breeding, it is necessary to consider improving the embryo culture conditions and simplifying distinction method of hybrids. In embryo culture conditions, germination rates are influenced by the timing of embryo excision after crossing, phytohormone concentration/combinations and sucrose concentration (Chen and Mii 2012; Motohashi et al 2008;Tar et al 2018), so we will improve of the phytohormone concentrations/combinations, and the timing of embryo removal in the crossing A. frutescens with R. gayanum in the future.…”

mentioning

confidence: 99%

Development of the sequence-characterized amplified region (SCAR) marker for distinction of intergeneric hybrids between <i>Argyranthemum frutescens</i> (L.) Sch. Bip. and <i>Rhodanthemum gayanum</i> (Coss. & Durieu) B.H. Wilcox, K. Bremer & Humphries

Muto

Kuronuma

Ando

et al. 2020

Plant Biotechnology

View full text Add to dashboard Cite

Distinction of intergeneric hybrids between Argyranthemum frutescens (L.) Sch. Bip. and Rhodanthemum gayanum (Cross. & Durieu) B.H. Wilcox, K. Bremer & Humphries is important for the development of new intergeneric hybrids.The aim of this study was to develop a simple and cost-effective DNA marker to distinguish between a large number of putative hybrids of A. frutescens × R. gayanum during primary selection. We designed sequence-characterized amplified region (SCAR) markers based on the genetic sequences of the internal transcribed spacer (ITS) region for A. frutescens and R. gayanum. Specific SCAR markers for A. frutescens amplified bands in A. frutescens and hybrids but not in R. gayanum. In contrast, specific SCAR markers for R. gayanum amplified bands in R. gayanum and hybrids but not in A. frutescens. Additionally, we confirmed the reproducibility of the SCAR marker using 31 cultivars. The findings demonstrated that these SCAR markers could be used to distinguish putative hybrids between A. frutescens and R. gayanum. This is the first report of the development of a SCAR marker to facilitate distinction of these hybrids.

show abstract

Adventitious embryo formation derived from zygotic embryos in Cycas revoluta

Cited by 2 publications

References 10 publications

Simplified Regeneration Protocol for Cycas revoluta Thunb. Mature Zygotic Embryos

Simplified Regeneration Protocol for Cycas revoluta Thunb. Mature Zygotic Embryos

Development of the sequence-characterized amplified region (SCAR) marker for distinction of intergeneric hybrids between <i>Argyranthemum frutescens</i> (L.) Sch. Bip. and <i>Rhodanthemum gayanum</i> (Coss. & Durieu) B.H. Wilcox, K. Bremer & Humphries

Contact Info

Product

Resources

About