Adventitious bud regeneration from leaf explants of Platanus occidentalis L. and genetic stability assessment

Sun, Yipeng; Zhao, Yanling; Wang, Xiaojuan; Qiao, Guirong; Chen, Guoliang; Yang, Ye; Zhou, Jing; Jin, Liang; Zhuo, Renying

doi:10.1007/s11738-008-0196-9

Cited by 18 publications

(9 citation statements)

References 60 publications

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“…The most important factor influencing bud differentiation is the type of growth regulator used especially the type of cytokinin [2,13]. Its concentration in the culture medium is critical for shoot organogenesis [14]. The promoter effect of BAP in the induction of multiple shoots has previously been reported [15].…”

Section: Discussionmentioning

confidence: 99%

In vitro propagated Mentha rotundifolia (L.) Huds and antioxidant activity of its essential oil

Benahmed

Harfi

Benbelkacem

et al. 2018

Current Issues in Pharmacy and Medical Sciences

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Mentha rotundifolia (L.) Huds is an aromatic plant used for its medicinal values. This study aims to select appropriate conditions for in vitro propagation of M. rotundifolia (L.) Huds and to evaluate yield and antioxidants activity of its essential oils (EOs). The explants were cultured on Murashige and Skoog (MS) medium containing different concentrations of growth regulators 6-benzylaminopurine (BAP) and Gibberellin (GA3). Hydrodistillated EOs obtained from acclimatized and mother plant, were evaluated for their antioxidant activity. Tests were performed on DPPH free radical-scavenging, ABTS and CUPRAC assays. Shoot induction and multiplication were successfully carried out on MS medium supplemented with the following hormones combinations: 1 mg/l BAP, 1 mg/l GA3 and 0.5 mg/l BAP, 0.5 mg/l GA3, respectively. Stem length, nodes and leaves number measured from development vitroplant were 6.89 cm, 5.22 nodes and 11.92 leaves per vitroplant, respectively. In vitro rooted plants were successfully acclimatized at a temperature of 23 ± 2°C and a long day photoperiod with a total survival rate exceeding 95%. EO yield of acclimated plant varied between (0.88-1.49 ml/100 g dry matter) compared to wild plant (0.73 ml/100 g dry matter). The antioxidant potential of EOs from acclimated plant showed on DPPH free radical-scavenging, ABTS and CUPRAC assays values of (IC50: 4.18-24.93 mg/ml), (IC50: 0.51-1.56 mg/ml) and (A0.50: 0.34-2.71 mg/ml), respectively. In contrast, the wild plant exhibited on the same tests the values of (IC50: 10.35 mg/ml), (IC50: 0.12 mg/ml) and (A0.50: 0.99 mg/ml), respectively. The results suggest that micropropagation of M. rotundifolia (L.) Huds can be an interesting alternative for producing important plant material with the possibility to modulate EO yield and its antioxidant potential for future commercial purposes.

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Section: Discussionmentioning

confidence: 99%

In vitro propagated Mentha rotundifolia (L.) Huds and antioxidant activity of its essential oil

Benahmed

Harfi

Benbelkacem

et al. 2018

Current Issues in Pharmacy and Medical Sciences

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“…Regeneration via adventitious shoots in woody species has been reported in Dalbergia latifolia (Lakshmisita et al 1986), Aegle marmelos (Islam et al 1993), Sesbania grandiflora (Detrez et al 1994), Liquidambar styraciflua (Kim et al 1997), Prunus avium (Hammatt and Grant 1998), D. sissoo (Pradhan et al 1998;Chand et al 2002), Azadirachta indica (Eswara et al 1998;Sharma et al 2002), Salix nigra (Lyyra et al 2006), Paulownia tomentosa (Corredoira et al 2008), Platanus occidentalis (Sun et al 2009), Jatropha curcas (Kumar et al 2010), Cassia angustifolia (Siddique et al 2010),Tabebuia donnell-smithii rose (GonzalezRodriguez et al 2010), Pterocarpus marsupium (Husain et al 2010), and Albizia lebbeck (Perveen et al 2011). …”

Section: Propagation By Adventitious Shoot Organogenesismentioning

confidence: 96%

Review of Literature

Varshney¹,

Anis²

2014

Trees: Propagation and Conservation

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Gottlieb Haberlandt (Math Naturwiss 111:69-92), a German plant physiologist, for the first time initiated the work on tissue culture. His work arose as a research tool and attempts were made to culture the isolated, fully differentiated cells in nutrient medium in vitro as early as 1898. The theoretical basis of tissue culture lies in the cell theory given by Schleiden and Schwann (1838-1839). Practically, this technique stands on the concept of "totipotency," i.e., each cell has the ability to regenerate into a new plant. The field finds a wide range of applications starting from mass clonal propagation to plant improvement, molecular biology, bio-processing as well as a basic research tool. It has advanced the production in forestry and agriculture to many folds. There are a number of reviews published on tissue culture of woody and tree species which provide the wide-ranging micropropagation reports of various plant species. In this way, this chapter highlights the current review in tree tissue culture in vitro and micropropagation of other valuable plants, their significance, and the wide scope existing for investigations on mass multiplication and conservation of these plants.

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“…Taylor et al, (1995) and Choudhuri et al, (2009) reported that PCR-based techniques such as RAPD and ISSR markers were successfully employed for the detection of variations at the genome level among regenerants. Since RAPD and ISSR techniques are simple and cost effective, it has been widely used by many researchers to assess genetic stability in micropropagated plants (Agnihotri et al, 2009; Chalageri and Babu 2012; Paul et al, 2010;Sun et al, 2009). Hence, in the present study we employed RAPD and ISSR analysis to check the genetic uniformity among the micropropagated plants and mother plant.…”

Section: Rapd and Issr Analysis Of Regenerated Plantsmentioning

confidence: 99%

Genetic Fidelity in Micropropagated Plantlets of Pimpinella tirupatiensis-an Endemic and Threatened Medicinal Plant Using RAPD and ISSR Markers

Srilakshmi¹,

Ugraiah²,

Gayatri³

et al. 2016

IJB

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Tissue cultured Pimpinella tirupatiensis plantlets were subjected to assessment of genetic stability considering the fact that associated in vitro stress might result in breakdown of control mechanism causing instability of the genome. We have used two DNA based molecular markers to assess the genetic fidelity of in vitro regenerated Pimpinella tirupatiensis through shoot tip from in vitro raised seedlings. The shoot tips upon transfer to MS medium containing different concentrations and combinations of 6- benzyl aminopurine (BAP) kinetin (KN), 2- isopentyl adenine (2- ip), α- napthalene acetic acid (NAA) and indole 3- acetic acid (IAA). The best morphogenic response was observed on MS medium fortified with BA (13.31 µM) and NAA (2.69 µM) which exhibited the highest regeneration frequency (90%), the maximum number of shoots/explants (6.50 ± 0.91) and shoot length (3.20 ± 0.20) within 5 weeks. Rooting was achieved within 15 days of shoot implantation on ½ strength MS media fortified with BAP (13.31 µM) and IBA (9.8 µM). The rooted plantlets were successfully acclimatized with 85% survival rate. Out of 20 RAPD and 3 ISSR primers screened, only 6 random amplified polymorphic DNA (RAPD) and all three inter simple sequence repeats (ISSR) primers produced clear reproducible and scorable bands. All banding profiles from micropropagated plants were monomorphic and similar to the mother plant indicating an absence of noticeable genetic variation in the regenerated plantlets. This study is of high significance as these could be commercially utilized for large scale production of true-to-type plantlets in Pimpinella tirupatiensis.

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Adventitious bud regeneration from leaf explants of Platanus occidentalis L. and genetic stability assessment

Cited by 18 publications

References 60 publications

In vitro propagated Mentha rotundifolia (L.) Huds and antioxidant activity of its essential oil

In vitro propagated Mentha rotundifolia (L.) Huds and antioxidant activity of its essential oil

Review of Literature

Genetic Fidelity in Micropropagated Plantlets of Pimpinella tirupatiensis-an Endemic and Threatened Medicinal Plant Using RAPD and ISSR Markers

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