Adventitial Cell Atlas of wt (Wild Type) and ApoE (Apolipoprotein E)-Deficient Mice Defined by Single-Cell RNA Sequencing

Gu, Wenduo; Ni, Zhichao; Tan, Yuan Qing; Deng, Jiacheng; Zhang, Si Jin; Lv, Zi; Wang, Xiao Jian; Chen, Ting; Zhang, Zhongyi; Hu, Yanhua; Jing, Zhi‐Cheng; Xu, Qingbo

doi:10.1161/atvbaha.119.312399

Cited by 86 publications

(108 citation statements)

References 74 publications

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“…Other studies have focused on the non-EC compartments in the atherosclerotic plaques. Working on the ApoE −/− atherosclerosis mouse model, Gu and co-workers found that non-immune cells also appear in a pro-inflammatory and stem-/progenitor-like state that is typified by the expression of stem cell antigen 1 (SCA1) in SMCs [52]. Moreover, isolated SCA1 + SMCs had high CCL2 expression in vitro, consistent with the pro-inflammatory signature.…”

Section: Atherosclerosismentioning

confidence: 90%

“…Moreover, isolated SCA1 + SMCs had high CCL2 expression in vitro, consistent with the pro-inflammatory signature. Other cell types such as resident macrophages were also in an activated pro-inflammatory state [52]. While Western diet or ApoE knockout mouse models do not result in formation of progressive atherosclerotic lesions, these models nevertheless provide important insights into its early development and first-wave responses of ECs and SMCs to atherosclerosis development.…”

Section: Atherosclerosismentioning

confidence: 99%

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Vascular Homeostasis and Inflammation in Health and Disease—Lessons from Single Cell Technologies

Bondareva

Sheikh

2020

IJMS

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The vascular system is critical infrastructure that transports oxygen and nutrients around the body, and dynamically adapts its function to an array of environmental changes. To fulfil the demands of diverse organs, each with unique functions and requirements, the vascular system displays vast regional heterogeneity as well as specialized cell types. Our understanding of the heterogeneity of vascular cells and the molecular mechanisms that regulate their function is beginning to benefit greatly from the rapid development of single cell technologies. Recent studies have started to analyze and map vascular beds in a range of organs in healthy and diseased states at single cell resolution. The current review focuses on recent biological insights on the vascular system garnered from single cell analyses. We cover the themes of vascular heterogeneity, phenotypic plasticity of vascular cells in pathologies such as atherosclerosis and cardiovascular disease, as well as the contribution of defective microvasculature to the development of neurodegenerative disorders such as Alzheimer’s disease. Further adaptation of single cell technologies to study the vascular system will be pivotal in uncovering the mechanisms that drive the array of diseases underpinned by vascular dysfunction.

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Section: Atherosclerosismentioning

confidence: 90%

Section: Atherosclerosismentioning

confidence: 99%

Vascular Homeostasis and Inflammation in Health and Disease—Lessons from Single Cell Technologies

Bondareva

Sheikh

2020

IJMS

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“…They play a role in vasomotion, homeostasis and permeability of the vasculature [11]. The heterogeneity of perivascular cells has been unclear for many years, but recent single-cell RNA-sequencing data suggest that various, previously unknown, perivascular mesenchymal populations exist [28,29]. We have reported that Gli1 marks a specific perivascular cell type that drives cardiac fibrosis and vascular calcification [30,31].…”

Section: Cardiac Fibrosismentioning

confidence: 95%

Cardiac Remodeling in Chronic Kidney Disease

Kaesler

Babler

Floege

et al. 2020

Toxins

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Cardiac remodeling occurs frequently in chronic kidney disease patients and affects quality of life and survival. Current treatment options are highly inadequate. As kidney function declines, numerous metabolic pathways are disturbed. Kidney and heart functions are highly connected by organ crosstalk. Among others, altered volume and pressure status, ischemia, accelerated atherosclerosis and arteriosclerosis, disturbed mineral metabolism, renal anemia, activation of the renin-angiotensin system, uremic toxins, oxidative stress and upregulation of cytokines stress the sensitive interplay between different cardiac cell types. The fatal consequences are left-ventricular hypertrophy, fibrosis and capillary rarefaction, which lead to systolic and/or diastolic left-ventricular failure. Furthermore, fibrosis triggers electric instability and sudden cardiac death. This review focuses on established and potential pathophysiological cardiorenal crosstalk mechanisms that drive uremia-induced senescence and disease progression, including potential known targets and animal models that might help us to better understand the disease and to identify novel therapeutics.

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“…In rodents model of arterial injury, perivascular administration of mesenchymal cells overexpressing VEGF around injured arteries increased adventitial blood vessels and macrophage recruitment [ 31 ]. In a model of atherosclerosis, single-cell RNA sequencing of aortic adventitia from wild type and ApoE (apolipoprotein E)-deficient mice identified a pro-inflammatory subset of adventitial fibroblasts expressing chemokines such as CCL2, essential for the recruitment of monocytes, further suggesting a causal role for adventitial fibroblasts in inflammation [ 32 ] ( Figure 1 b).…”

Section: Interaction With Immune Cells In the Perivascular Spacementioning

confidence: 99%

Mesenchymal perivascular cells in immunity and disease

Benabid

Peduto

2020

Current Opinion in Immunology

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Highlights Perivascular mesenchymal cells include pericytes, adventitial fibroblasts and mesenchymal stromal cells. Perivascular mesenchymal cells have roles in the recruitment and/or activity of specific immune populations. Subsets of perivascular mesenchymal cells act as progenitors for specialized stromal cells essential in repair and immunity. Dysregulation of the perivascular niche contribute to chronic inflammation and fibrosis.

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Adventitial Cell Atlas of wt (Wild Type) and ApoE (Apolipoprotein E)-Deficient Mice Defined by Single-Cell RNA Sequencing

Abstract: Supplemental Digital Content is available in the text.

Cited by 86 publications

References 74 publications

Vascular Homeostasis and Inflammation in Health and Disease—Lessons from Single Cell Technologies

Vascular Homeostasis and Inflammation in Health and Disease—Lessons from Single Cell Technologies

Cardiac Remodeling in Chronic Kidney Disease

Mesenchymal perivascular cells in immunity and disease

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