AdVEGF<sub>165</sub> gene transfer increases survival in overdimensioned skin flaps

Giunta, Riccardo E.; Holzbach, T.; Taskov, C.; Holm, Per Sonne; Konerding, Moritz A.; Schams, D.; Biemer, E.; Gänsbacher, Bernd

doi:10.1002/jgm.675

Cited by 48 publications

(47 citation statements)

References 34 publications

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“…[34][35][36][37] The benefits of enhanced angiogenesis have primarily been shown in models of impaired wound healing 28,32,33 or severe injury. [29][30][31] In contrast, several reports have shown that modulation of angiogenesis does not affect epidermal healing rates or overall wound closure to a great degree, [38][39][40][41][42][43] and some studies have even reported enhanced healing with reduced angiogenesis. 44 Thus, although there are many studies examining angiogenesis and wound repair, the degree to which angiogenesis actually facilitates healing under normal circumstances is still not known.…”

Section: Discussionmentioning

confidence: 97%

“…It is not surprising then, that many studies have found angiogenesis to be beneficial for wound repair. Stimulation of angiogenesis can enhance healing rates, [27][28][29][30][31][32][33] whereas a reduction in angiogenesis can impair the same. [34][35][36][37] The benefits of enhanced angiogenesis have primarily been shown in models of impaired wound healing 28,32,33 or severe injury.…”

Section: Discussionmentioning

confidence: 99%

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Regulation of scar formation by vascular endothelial growth factor

Wilgus

Ferreira

Oberyszyn

et al. 2008

Laboratory Investigation

276

216

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Regulation of scar formation by vascular endothelial growth factor

Wilgus

Ferreira

Oberyszyn

et al. 2008

Laboratory Investigation

276

216

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“…VEGF, which is physiologically secreted by fibroblasts and inflammatory cells [6], has also been shown to influence *E-mail: e.maeda@eng.hokudai.ac.jp (EM) angiogenic process [7,8]. It was reported that VEGF has the ability of changing EC shape during vascular lumen formation [9].…”

Section: Introductionmentioning

confidence: 99%

Development of fibrin gel-microgroove model for microvascularization by endothelial cells

et al. 2015

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This study was performed to develop a new experimental device with a fibrin gel-microgroove structure for study of microvascularization by endothelial cells (ECs). The effects of the width of microgrooves, initial cell seeding density and a supplementation of vascular endothelial growth factor (VEGF) on in vitro microvasculaization of ECs were examined. ECs were cultured in a fibrin gel formed on a polydimethylsiloxane microgroove substrate, with the microgroove width of 50, 100, 150 and 200 μm. ECs were elongated and sprouted within the gel in all the four types of microgrooves. In addition, multicellular network by connected cell branches were frequently observed in 100-μm microgrooves. Both high initial cell density and VEGF demonstrated significant promotional effects on morphology changes. The findings indicate that microgroove structure serves as a geometrical constraint for ECs, with a promotional effect on angiogenic responses of ECs, and thus, it can be used as an experimental model in the study of in vitro vascularization.

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“…Flaps with ratios greater than 2:1 have been shown to exhibit ischemia in distal regions due to decreased proximity to the pedicled end [12,13]. Studies conducted in rats bearing overdimensioned 4:1 random-pattern fl aps demonstrated ischemia in distal halves of fl aps that, over time, led to fl ap necrosis in those regions [12,13].…”

mentioning

confidence: 99%

“…Studies conducted in rats bearing overdimensioned 4:1 random-pattern fl aps demonstrated ischemia in distal halves of fl aps that, over time, led to fl ap necrosis in those regions [12,13]. In this study we create over-dimensioned 4:1 random-pattern local fl aps in a murine model, anticipating a loss of perfusion in the distal halves of fl aps.…”

mentioning

confidence: 99%

Effects of Neoadjuvant Chemotherapy and Radiotherapy on Flap Perfusion in a Novel Mouse Model Using Standard Clinical Assessment and Near-Infrared Fluorescence Angiography

Ramesh¹,

Moore²,

Patel³

et al. 2017

Arch Otolaryngol Rhinold

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IntoductionThe clinical management of locally advanced cancers of the head and neck pose a signifi cant therapeutic challenge [1]. Despite advances in the fi eld, including the routine use of microvascular free tissue transfers in the reconstruction of defects, fl ap failure persists as a troublesome complication 2 . The underlying factors that infl uence the development of fl ap failure are varied and complex, including comorbid systemic diseases, positive surgical margins, lymph node metastases, and prior radiotherapy and/or chemotherapy [1][2][3].Radiation and chemotherapy are often administered neoadjuvantly, or prior to surgical intervention, to gauge Abstract Purpose: Minimizing surgical morbidity after local fl ap reconstruction is important in the management of cutaneous defects. Controversy exists in current literature regarding the effects of radiation and chemotherapy on fl ap perfusion. Neoadjuvant treatments can damage the microvasculature of the surgical bed through fi brosis, endothelial cell damage, and reduced cell proliferation, which collectively increase the likelihood of postoperative fl ap failure. The aim of this study is to examine the effects of neoadjuvant radiation and chemotherapy on skin fl ap perfusion.Methods: Animals were divided into three groups: control (no treatment, n=4), radiation group (36-Gy administered to dorsal skin, n=4), and chemotherapy group (2 mg/kg IP cisplatin, n=4). Treatments were performed 15 days prior to random-pattern dorsal fl ap surgery, with a fl ap length-to-width ratio of 4:1 (4x1cm2). Flap perfusion was assessed via laser-assisted (Luna, Novadaq) indocyanine green dye angiography and standard clinical assessment. Results:Fluorescence imaging was used to quantify fl ap perfusion as a fraction of healthy skin perfusion. Chemotherapy group fl aps had poorer distal end perfusion than radiation or control group fl aps (56% vs. 69% and 71%, respectively) on post-operative day (POD) 1. Clinical assessment of fl ap perfusion by experienced surgeons on POD2 found chemotherapy group fl aps to be least viable. By POD5, 100% of chemotherapy group fl aps had experienced complete fl ap loss as measured by clinical evaluation and perfusion imaging. Conclusion:Flaps receiving neoadjuvant chemotherapy performed worse than those receiving local radiotherapy or no treatment. We demonstrate the detrimental effect of neoadjuvant chemotherapy on fl ap viability in this preclinical murine model.

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AdVEGF₁₆₅ gene transfer increases survival in overdimensioned skin flaps

Cited by 48 publications

References 34 publications

Regulation of scar formation by vascular endothelial growth factor

Regulation of scar formation by vascular endothelial growth factor

Development of fibrin gel-microgroove model for microvascularization by endothelial cells

Effects of Neoadjuvant Chemotherapy and Radiotherapy on Flap Perfusion in a Novel Mouse Model Using Standard Clinical Assessment and Near-Infrared Fluorescence Angiography

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AdVEGF165 gene transfer increases survival in overdimensioned skin flaps

Cited by 48 publications

References 34 publications

Regulation of scar formation by vascular endothelial growth factor

Regulation of scar formation by vascular endothelial growth factor

Development of fibrin gel-microgroove model for microvascularization by endothelial cells

Effects of Neoadjuvant Chemotherapy and Radiotherapy on Flap Perfusion in a Novel Mouse Model Using Standard Clinical Assessment and Near-Infrared Fluorescence Angiography

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AdVEGF₁₆₅ gene transfer increases survival in overdimensioned skin flaps