Advantages and limitations of various treatment chamber designs for reversible and irreversible electroporation in life sciences

Zand, Elena; Schottroff, Felix; Steinacker, Elisabeth; Mae-Gano, Jennifer; Schoenher, Christoph; Wimberger, Terje; Wassermann, Klemens J.; Jaeger, Henry

doi:10.1016/j.bioelechem.2021.107841

Cited by 12 publications

(6 citation statements)

References 94 publications

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“…Electroporation of the microbial cell membrane is induced by generating high-intensity electric fields (kV cm −1 ) for repetitive short time periods (µs). The formation of pores can either be reversible or irreversible, depending on the interplay of various conditions such as treatment intensity, electrical conductivity, and electrode configuration [ 62 ]. Permanent pores can result in membrane integrity loss and uncontrolled molecule transfer through microbial cell membranes [ 210 ].…”

Section: Strategies To Overcome Limitations and To Improve Detection Methodsmentioning

confidence: 99%

“…For food-related research, FCM is mainly used for the performance testing of food preservation or disinfection approaches, i.e., sodium hypochlorite or peracetic acid disinfection, ultraviolet light (UV-C), supercritical CO 2 pasteurization, ohmic heating applications, non-thermal inactivation technologies, including pulsed electric fields and cold atmospheric pressure plasma treatment, as well as natural preservatives such as essential oils [ 47 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 ]. The most commonly investigated microorganism was E. coli [ 3 , 55 , 56 , 57 , 59 , 60 , 61 , 63 , 64 , 65 , 66 ].…”

Section: Non-specific State-of-the-art Flow Cytometric Applications For Detection and Monitoringmentioning

confidence: 99%

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Potential of Flow Cytometric Approaches for Rapid Microbial Detection and Characterization in the Food Industry—A Review

Zand

Froehling

Schoenher

et al. 2021

Foods

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As microbial contamination is persistent within the food and bioindustries and foodborne infections are still a significant cause of death, the detection, monitoring, and characterization of pathogens and spoilage microorganisms are of great importance. However, the current methods do not meet all relevant criteria. They either show (i) inadequate sensitivity, rapidity, and effectiveness; (ii) a high workload and time requirement; or (iii) difficulties in differentiating between viable and non-viable cells. Flow cytometry (FCM) represents an approach to overcome such limitations. Thus, this comprehensive literature review focuses on the potential of FCM and fluorescence in situ hybridization (FISH) for food and bioindustry applications. First, the principles of FCM and FISH and basic staining methods are discussed, and critical areas for microbial contamination, including abiotic and biotic surfaces, water, and air, are characterized. State-of-the-art non-specific FCM and specific FISH approaches are described, and their limitations are highlighted. One such limitation is the use of toxic and mutagenic fluorochromes and probes. Alternative staining and hybridization approaches are presented, along with other strategies to overcome the current challenges. Further research needs are outlined in order to make FCM and FISH even more suitable monitoring and detection tools for food quality and safety and environmental and clinical approaches.

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Section: Strategies To Overcome Limitations and To Improve Detection Methodsmentioning

confidence: 99%

Section: Non-specific State-of-the-art Flow Cytometric Applications For Detection and Monitoringmentioning

confidence: 99%

Potential of Flow Cytometric Approaches for Rapid Microbial Detection and Characterization in the Food Industry—A Review

Zand

Froehling

Schoenher

et al. 2021

Foods

Self Cite

View full text Add to dashboard Cite

show abstract

“…Usage of hypoosmolar buffers that swell the cell has also demonstrated better EP performance . Buffers with low conductivity increased cell viability without altering reversible EP efficiency because the low current reduced the harmful Joule heating. − High conductivity buffer performance is limited by pulse generator hardware, which may not be able to handle high currents to matched the desired energy input …”

Section: Mechanism Of Electroporationmentioning

confidence: 99%

“…92−94 High conductivity buffer performance is limited by pulse generator hardware, which may not be able to handle high currents to matched the desired energy input. 95 2.2.2. Electric Pulse Parameters.…”

Section: Performance Affecting Factorsmentioning

confidence: 99%

Recent Advances in Microscale Electroporation

2022

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Electroporation (EP) is a commonly used strategy to increase cell permeability for intracellular cargo delivery or irreversible cell membrane disruption using electric fields. In recent years, EP performance has been improved by shrinking electrodes and device structures to the microscale. Integration with microfluidics has led to the design of devices performing static EP, where cells are fixed in a defined region, or continuous EP, where cells constantly pass through the device. Each device type performs superior to conventional, macroscale EP devices while providing additional advantages in precision manipulation (static EP) and increased throughput (continuous EP). Microscale EP is gentle on cells and has enabled more sensitive assaying of cells with novel applications. In this Review, we present the physical principles of microscale EP devices and examine design trends in recent years. In addition, we discuss the use of reversible and irreversible EP in the development of therapeutics and analysis of intracellular contents, among other noteworthy applications. This Review aims to inform and encourage scientists and engineers to expand the use of efficient and versatile microscale EP technologies.

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“…An adequately designed treatment chamber is essential to bring about a much powerful and uniform electric field to either kill or weaken the microorganisms in the juices [14][15][16][17]. An increased uniformity is achieved by altering the dimensions of the treatment chamber and its geometry [18].…”

Section: Introductionmentioning

confidence: 99%

Simulation Study of Coaxial Treatment Chambers for PEF Pasteurization: A Critical Review

2021

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A pulsed electric field (PEF) produces pasteurized liquid foods with fresh and nutritional properties. The treatment chamber is a crucial part of the PEF processing system where a high voltage is applied, producing an electric field to treat the liquid foods. The proper construction of the treatment chamber regulates the distribution of the electrical field inside the treatment zone. Mixing of liquid inside the treatment zone is an effective tool to overcome this heterogeneous effect. The coaxial treatment chamber offers a heterogeneous electric field and temperature distribution inside the treatment zone. A helical insulator inside the coaxial treatment chamber provides a mixing effect. In this research, a numerical simulation was done to measure the electric field in different geometries of treatment chambers at different flow rates. The simulation aimed to optimize the new coaxial treatment chamber design. The modelling findings showed homogeneous electrical field strength in the helical treatment chamber. This study provides new insights for industrial-scale setup using multiple helical chambers in a continuous flow PEF treatment.

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Advantages and limitations of various treatment chamber designs for reversible and irreversible electroporation in life sciences

Cited by 12 publications

References 94 publications

Potential of Flow Cytometric Approaches for Rapid Microbial Detection and Characterization in the Food Industry—A Review

Potential of Flow Cytometric Approaches for Rapid Microbial Detection and Characterization in the Food Industry—A Review

Recent Advances in Microscale Electroporation

Simulation Study of Coaxial Treatment Chambers for PEF Pasteurization: A Critical Review

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