Advantages and limitations of using cell viability assays for 3D bioprinted constructs
Sofia Avnet,
Gemma Di Pompo,
Giorgia Borciani
et al.
Abstract:Bioprinting shows promise for bioengineered scaffolds and 3D disease models, but assessing the viability of embedded cells is challenging. Conventional assays are limited by the technical problems that derive from using multi-layered bioink matrices dispersing cells in three dimensions. 
In this study, we tested bioprinted osteogenic bioinks as a model system. Alginate- or gelatin-based bioinks were loaded with/without ceramic microparticles and osteogenic cells (bone tumour cells, with or without norm… Show more
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