Advantages and application of label-free detection assays in drug screening

Abstract

Microplate-based optical biosensors are enabling a variety of cell-based assays, inhibition assays, protein-protein binding assays and protein-small molecule binding assays to be performed with high-throughput and high sensitivity.

Integrated Approach to Structure-Based Enzymatic Drug Design: Molecular Modeling, Spectroscopy, and Experimental Bioactivity

“…14,419,563−579 Several drug design-related review articles have also included biophysical methods as a section of the manuscript. 569 615 Raman, 616−618 and surface plasmon resonance, 580,619−626 and synchrotron radiation spectroscopy. 627 The following specific nonspectrocopic biophysical methods have recently been reviewed: capillary electrophoresis, 628 centrifugation, 629,630 electrochemical methods, 631 cryoelectron microscopy, 632 labeling techniques (spin labeling, double labeling, fluorescent labeling, triplet labeling, Moessbauer spectroscopy), 563 mass spectrometry, 633−637 molecular imprinting, 584,638 nanoparticles, 624,639−644 optical tweezers, 645 scanning electrochemical microscopy, 646 size characterization, 647 thermophoresis, 648 and X-ray crystallography 649−655 as well as crystal growing techniques.…”

High Throughput Screening Approach to Lead Discovery

“…Due to their relatively low throughput most of these technologies are currently confined to specialist assays that are used mainly for compound triaging and mode-of-action studies. However, the level of interest and activity in this field promises rapid developments and greater impact on HTS in the not too distant future [144].…”

Using label-free screening technology to improve efficiency in drug discovery

“…In this review, we focus on HTS predominantly using cell-based assays. There are numerous reviews [12][13][14][15][16] on the use of label-free biosensors for biochemical assays and these are, therefore, not reiterated in this review. We also highlight some of the drawbacks of these technologies and technical issues to consider when using different platforms.…”