Advancing the enzymatic toolkit for 2′-fluoro arabino nucleic acid (FANA) manipulation: phosphorylation, ligation, replication, and templating RNA transcription

Abstract

Scheme of the amplification of DNA–FANA chimera via single-fNTP substitutional PCR, the synthesis of RNA from DNA–FANA chimeric templates via IVT, followed by the activation of the fluorescent spinach aptamer upon the addition of DFHBI.