Advancing functional genetics throughAgrobacterium-mediated insertional mutagenesis and CRISPR/Cas9 in the commensal and pathogenic yeastMalassezia furfur
Abstract:Malasseziaencompasses a monophyletic group of basidiomycetous yeasts naturally found on the skin of humans and other animals.Malasseziaspecies have lost genes for lipid biosynthesis, and are therefore lipid-dependent and difficult to manipulate under laboratory conditions. In this study we applied a recently-developedAgrobacterium tumefaciens-mediated transformation protocol to perform T-DNA random insertional mutagenesis inMalassezia furfur. A total of 767 transformants were screened after exposure to 10 diff… Show more
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