Advancing animal models of neoplasia through in vivo bioluminescence imaging

Edinger, Matthias; Cao, Yu; Hornig, Yvette S.; Jenkins, Darlene E.; Verneris, Michael R.; Bachmann, Michael; Negrin, Robert S.; Contag, Christopher H.

doi:10.1016/s0959-8049(02)00410-0

Cited by 205 publications

(146 citation statements)

References 36 publications

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“…30 Thus, the surgical resection of primary tumors performed in our study not only mimics therapeutic intervention in the clinic but also removes the intense bioluminescence focus thus enabling sensitive monitoring of metastasis development in vivo. Immediately after primary tumor removal no signs of secondary lesions were observed.…”

Section: In Vivo and Ex Vivo Optical Monitoring Of The Development Ofmentioning

confidence: 98%

Modulating metastasis by a lymphangiogenic switch in prostate cancer

Bråkenhielm

Burton

Johnson

et al. 2007

Intl Journal of Cancer

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Prostate cancer dissemination is difficult to detect in the clinic, and few treatment options exist for patients with advanced-stage disease. Our aim was to investigate the role of tumor lymphangiogenesis during metastasis. Further, we implemented a noninvasive molecular imaging technique to facilitate the assessment of the metastatic process. The metastatic potentials of several human prostate cancer xenograft models, LAPC-4, LAPC-9, PC3 and CWR22Rv-1 were compared. The cells were labeled with luciferase, a bioluminescence imaging reporter gene, to enable optical imaging. After tumor implantation the animals were examined weekly during several months for the appearance of metastases. Metastatic lesions were confirmed by immunohistochemistry. Additionally, the angiogenic and lymphangiogenic profiles of the tumors were characterized. To confirm the role of lymphangiogenesis in mediating metastasis, the low-metastatic LAPC-9 tumor cells were engineered to overexpress VEGF-C, and the development of metastases was evaluated. Our results show CWR22Rv-1 and PC3 tumor cell lines to be more metastatic than LAPC-4, which in turn disseminates more readily than LAPC-9. The difference in metastatic potential correlated with the endogenous production levels of lymphangiogenic growth factor VEGF-C and the presence of tumor lymphatics. In agreement, induced overexpression of VEGF-C in LAPC-9 enhanced tumor lymphangiogenesis leading to the development of metastatic lesions. Taken together, our studies, based on a molecular imaging approach for semiquantitative detection of micrometastases, point to an important role of tumor lymphatics in the metastatic process of human prostate cancer. In particular, VEGF-C seems to play a key role in prostate cancer metastasis. ' 2007 Wiley-Liss, Inc.

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Section: In Vivo and Ex Vivo Optical Monitoring Of The Development Ofmentioning

confidence: 98%

Modulating metastasis by a lymphangiogenic switch in prostate cancer

Bråkenhielm

Burton

Johnson

et al. 2007

Intl Journal of Cancer

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“…BLI is an affordable imaging modality with high sensitivity and specificity (Edinger et al, 2002). The shortcoming of using BLI is the limit of penetration depth to *1 cm, which makes it practical for only small-animal imaging (Choy et al, 2003;Dothager et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

Visualization and quantification of simian immunodeficiency virus-infected cells using non-invasive molecular imaging

Song

Cai

White

et al. 2015

Journal of General Virology

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In vivo imaging can provide real-time information and three-dimensional (3D) non-invasive images of deep tissues and organs, including the brain, whilst allowing longitudinal observation of the same animals, thus eliminating potential variation between subjects. Current in vivo imaging technologies, such as magnetic resonance imaging (MRI), positron emission tomography-computed tomography (PET-CT) and bioluminescence imaging (BLI), can be used to pinpoint the spatial location of target cells, which is urgently needed for revealing human immunodeficiency virus (HIV) dissemination in real-time and HIV-1 reservoirs during suppressive antiretroviral therapy (ART). To demonstrate that in vivo imaging can be used to visualize and quantify simian immunodeficiency virus (SIV)-transduced cells, we genetically engineered SIV to carry different imaging reporters. Based on the expression of the reporter genes, we could visualize and quantify the SIV-transduced cells via vesicular stomatitis virus glycoprotein pseudotyping in a mouse model using BLI, PET-CT or MRI. We also engineered a chimeric EcoSIV for in vivo infection study. Our results demonstrated that BLI is sensitive enough to detect as few as five single cells transduced with virus, whilst PET-CT can provide 3D images of the spatial location of as few as 10 000 SIV-infected cells. We also demonstrated that MRI can provide images with high spatial resolution in a 3D anatomical context to distinguish a small population of SIV-transduced cells. The in vivo imaging platform described here can potentially serve as a powerful tool to visualize lentiviral infection, including when and where viraemia rebounds, and how reservoirs are formed and maintained during latency or suppressive ART.

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“…38 This is low in comparison to imaging modalities that use more penetrating radiation such as positron emission tomography, single positron emission computed tomography and computed tomography. 37 Furthermore, the imaging data are in a two-dimensional format; so, the light detected is a consequence of all light emitted in a given plane, and further method development is needed to produce three-dimensional imaging/cross-sectional imaging. In addition, luciferase expression is under the control of the SV40 promoter which may have cell-type dependency.…”

Section: Discussionmentioning

confidence: 99%

Herpesvirus saimiri-based vector biodistribution using noninvasive optical imaging

et al. 2005

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Herpesvirus saimiri (HVS) is capable of infecting a range of human cell types with high efficiency and the viral genome persists as high copy number, circular, nonintegrated episomes which segregate to progeny upon cell division. This allows the HVS-based vector to stably transduce a dividing cell population and provide sustained transgene expression for an extended period of time both in vitro and in vivo. Here we assess the dissemination of HVS-based vectors in vivo following intravenous and intraperitoneal administration. Bioluminescence imaging of an HVS-based vector expressing luciferase demonstrates that the virus can infect and establish a persistent latent infection in a variety of mouse tissues. Moreover, the long-term in vivo maintenance of the HVS genome as a nonintegrated circular episome provided sustained expression of luciferase over a 10-week period. A particularly high level of transgene expression in the liver and the ability of HVS to infect and persist in hepatic stellate cells suggest that HVS-based vectors may have potential for the treatment of inherited and acquired liver diseases.

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Advancing animal models of neoplasia through in vivo bioluminescence imaging

Cited by 205 publications

References 36 publications

Modulating metastasis by a lymphangiogenic switch in prostate cancer

Modulating metastasis by a lymphangiogenic switch in prostate cancer

Visualization and quantification of simian immunodeficiency virus-infected cells using non-invasive molecular imaging

Herpesvirus saimiri-based vector biodistribution using noninvasive optical imaging

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