Advancing 2D fluorescence online monitoring in microtiter plates by separating scattered light and fluorescence measurement, using a tunable emission monochromator
Christoph Berg,
Selma Busch,
Muthia Dewi Alawiyah
et al.
Abstract:Online fluorescence monitoring has become a key technology in modern bioprocess development, as it provides in‐depth process knowledge at comparably low costs. In particular, the technology is widely established for high‐throughput microbioreactor cultivation systems, due to its noninvasive character. For microtiter plates, previously also multi‐wavelength 2D fluorescence monitoring was developed. To overcome an observed limitation of fluorescence sensitivity, this study presents a modified spectroscopic setup… Show more
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