Advances on the interaction of polypyridyl Cr(III) complexes with transporting proteins and its potential relevance in photodynamic therapy

Toneatto, Judith; García, Pablo Facundo; Argüello, Gustavo A.

doi:10.1016/j.jinorgbio.2011.07.013

Cited by 23 publications

(23 citation statements)

References 40 publications

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“…However, the electrostatic interaction cannot be excluded in view of the charges of the species involved. These results reveal that the [Cr(phen) 3 ] 3+ stabilizes holoTf with a strong entropic contribution as was found for the process of binding between [Cr(phen) 3 ] 3+ and apoTf [22] and [Cr(phen) 3 ] 3+ and albumin [33].…”

Section: Binding Modesupporting

confidence: 72%

“…It is possible that the binding of the Cr(III) complex may take place in hydrophobic patches present in Tf near iron binding site. Furthermore, Trp and Tyr residues may be involved, among others, as we have recently demonstrated for the [Cr(phen) 3 ] 3+ -apoTf association complex [22].…”

Section: Structural Changes Induced By [Cr(phen) 3 ] 3+mentioning

confidence: 62%

“…Previously, we demonstrated the binding properties of [Cr(phen) 2 (dppz)] 3+ and [Cr(phen) 3 ] 3+ complexes with apoTf [22]. The results clearly indicated that [Cr(phen) 2 (dppz)] 3+ and [Cr(phen) 3 ] 3+ bind efficiently to apoTf.…”

Section: Introductionmentioning

confidence: 76%

“…When [Cr(phen) 3 ] 3+ was added to the solution of holoTf (spectra b and c) no significant changes were observed in the spectra of the protein, indicating that the presence of the complex does not produce significant conformational changes in the protein. ApoTf has been studied in a previous work and showed no changes at all in the tertiary structure [22].…”

Section: Structural Changes Induced By [Cr(phen) 3 ] 3+mentioning

confidence: 93%

“…This result indicates that both mechanisms (static and dynamic) of quenching can take place in the protein association process. This behavior has been described in processes of binding between Cr(III) complexes with apoTf and albumins [22,33]. This type of deviation occurs when quencher molecules interact with tryptophan and tyrosine residues causing the fluorescence quenching; then they change their position within the fluorophore microenvironment making room for the other molecules of the quencher.…”

Section: Quenching Experimentsmentioning

confidence: 99%

See 4 more Smart Citations

Binding of [Cr(phen)3]3+ to transferrin at extracellular and endosomal pHs: Potential application in photodynamic therapy

García¹,

Toneatto²,

Silvero³

et al. 2014

Biochimica et Biophysica Acta (BBA) - General Subjects

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Section: Binding Modesupporting

confidence: 72%

Section: Structural Changes Induced By [Cr(phen) 3 ] 3+mentioning

confidence: 62%

Section: Introductionmentioning

confidence: 76%

Section: Structural Changes Induced By [Cr(phen) 3 ] 3+mentioning

confidence: 93%

Section: Quenching Experimentsmentioning

confidence: 99%

See 3 more Smart Citations

Binding of [Cr(phen)3]3+ to transferrin at extracellular and endosomal pHs: Potential application in photodynamic therapy

García¹,

Toneatto²,

Silvero³

et al. 2014

Biochimica et Biophysica Acta (BBA) - General Subjects

Self Cite

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New heteroleptic lanthanide complexes as multimodal drugs: Cytotoxicity studies, apoptosis, cell cycle analysis, DNA interactions, and protein binding

Arbănași

Musat

Mihăilă³

et al. 2020

Applied Organom Chemis

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We report herein the synthesis and characterization of four new heteroleptic complexes of Sm(III), Eu(III), Gd(III), and Tb(III) with the natural flavonoid 5-hydroxyflavone (primuletin) and 1,10-phenanthroline. According to the physicochemical characterization, the mononuclear complexes correspond to the general formula [Ln(OH) 2 L 1 L 2 ]ÁnH 2 O, where L 1 = C 15 H 9 O 3 (deprotonated 5-hydroxyflavone) and L 2 = C 12 H 8 N 2 (1,10-phenanthroline), Ln is the lanthanide cation, and n = 4 for Sm(III), 3.5 for Eu(III), 2 for Gd(III), and 3 for Tb(III). A six-coordinated distorted octahedron geometry was proposed for the complexes, and density functional theory (DFT) studies were used to calculate their optimized geometry. Cytotoxicity was studied using MTS assay on cervical, colorectal, colon, breast, and ovarian adenocarcinoma cell lines. Flow cytometry data were consistent with apoptotic cell death and disruption of the cell cycle in cervical and colon cancer cells. As a means to investigate the mechanism underlying the cytotoxic effects, the abilities of the complexes to interact with calf thymus DNA, human serum albumin, and transferrin have also been assessed. According to experimental and computational studies, the four lanthanide complexes act as DNA intercalators and bind strongly to serum proteins.

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Quantum Yield of DNA Strand Breaks under Photoexcitation of a Molecular Ruby

Wang

Ebel

Heinze

et al. 2023

Chemistry A European J

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Photodynamic therapy (PDT) used for treating cancer relies on the generation of highly reactive oxygen species, for example, singlet oxygen 1 O 2 , by light-induced excitation of a photosensitizer (PS) in the presence of molecular oxygen, inducing DNA damage in close proximity of the PS. Although many precious metal complexes have been explored as PS for PDT and received clinical approval, only recently, the potential of photoactive complexes of nonnoble metals as PS has been discovered. Using the DNA origami technology that can absolutely quantify DNA strand break cross sections, we assessed the potential of the luminescent transition metal complex [Cr(ddpd) 2 ] 3 + (ddpd = N,N '-dimethyl-N,N'-dipyridine-2-ylpyridine-2,6-diamine) to damage DNA in an air-saturated aqueous environment upon UV/Vis illumination. The quantum yield for strand breakage, that is, the ratio of DNA strand breaks to the number of absorbed photons, was determined to 1-4 %, indicating efficient transformation of photons into DNA strand breaks by [Cr(ddpd) 2 ] 3 + .

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Advances on the interaction of polypyridyl Cr(III) complexes with transporting proteins and its potential relevance in photodynamic therapy

Cited by 23 publications

References 40 publications

Binding of [Cr(phen)3]3+ to transferrin at extracellular and endosomal pHs: Potential application in photodynamic therapy

Binding of [Cr(phen)3]3+ to transferrin at extracellular and endosomal pHs: Potential application in photodynamic therapy

New heteroleptic lanthanide complexes as multimodal drugs: Cytotoxicity studies, apoptosis, cell cycle analysis, DNA interactions, and protein binding

Quantum Yield of DNA Strand Breaks under Photoexcitation of a Molecular Ruby

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