Advances on colorectal cancer 3D models: The needed translational technology for nanomedicine screening

Castro, Flávia; Pereira, Cassilda; Macedo, Maria Helena; Almeida, Andreia; Silveira, Maria José; Dias, Sofia; Cardoso, Ana Patrícia; Oliveira, Maria José; Sarmento, Bruno

doi:10.1016/j.addr.2021.06.001

Cited by 34 publications

(29 citation statements)

References 157 publications

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“…CRC 3D models have been recently reviewed, including hydrogels, patient-derived scaffolds, spheroids, organoids, microfluidic devices, and tumor and organ on-chip devices [ 22 , 24 , 25 , 118 , 119 ]. Three-dimensional organotypic models have shown a more realistic spatial conformation and polarization, increased cell junctions and cell-to-cell communication, as well as more accurate gene expression patterns reflecting truthfully in vivo pathophysiology, consequently increasing our understanding of CRC progression [ 118 ].…”

Section: Organotypic Models To Study Ecm-crc Cell Interactionsmentioning

confidence: 99%

“…Three-dimensional bioprinting technology has gained increased relevance by allowing the standardization of the scaffold model between experiments [ 118 ]. This technique is based on the computer-assisted deposition of bioinks, which can include cells, hydrogels and decellularized matrices into specified 3D conformations [ 129 ].…”

Section: Organotypic Models To Study Ecm-crc Cell Interactionsmentioning

confidence: 99%

“…Chen and colleagues developed a bionatural and slow-degrading collagen-polycaprolactone (PCL) bioprinted 3D CRC model, by co-culturing not only CAFs but also CRC cells and tumor-associated endothelial cells, mimicking the in vivo TME regarding proliferation, vascularization and adhesion [ 130 ]. The bioprinting scaffold-based 3D systems provide successful platforms for studying cell–cell interactions in CRC, but most of them still lack native components and fail to reproduce inter-patient ECM heterogenicity [ 118 ].…”

Section: Organotypic Models To Study Ecm-crc Cell Interactionsmentioning

confidence: 99%

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Decellularized Colorectal Cancer Matrices as Bioactive Scaffolds for Studying Tumor-Stroma Interactions

Marques-Magalhães

Cruz

Costa

et al. 2022

Cancers

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More than a physical structure providing support to tissues, the extracellular matrix (ECM) is a complex and dynamic network of macromolecules that modulates the behavior of both cancer cells and associated stromal cells of the tumor microenvironment (TME). Over the last few years, several efforts have been made to develop new models that accurately mimic the interconnections within the TME and specifically the biomechanical and biomolecular complexity of the tumor ECM. Particularly in colorectal cancer, the ECM is highly remodeled and disorganized and constitutes a key component that affects cancer hallmarks, such as cell differentiation, proliferation, angiogenesis, invasion and metastasis. Therefore, several scaffolds produced from natural and/or synthetic polymers and ceramics have been used in 3D biomimetic strategies for colorectal cancer research. Nevertheless, decellularized ECM from colorectal tumors is a unique model that offers the maintenance of native ECM architecture and molecular composition. This review will focus on innovative and advanced 3D-based models of decellularized ECM as high-throughput strategies in colorectal cancer research that potentially fill some of the gaps between in vitro 2D and in vivo models. Our aim is to highlight the need for strategies that accurately mimic the TME for precision medicine and for studying the pathophysiology of the disease.

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Section: Organotypic Models To Study Ecm-crc Cell Interactionsmentioning

confidence: 99%

Section: Organotypic Models To Study Ecm-crc Cell Interactionsmentioning

confidence: 99%

Section: Organotypic Models To Study Ecm-crc Cell Interactionsmentioning

confidence: 99%

See 1 more Smart Citation

Decellularized Colorectal Cancer Matrices as Bioactive Scaffolds for Studying Tumor-Stroma Interactions

Marques-Magalhães

Cruz

Costa

et al. 2022

Cancers

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“…Therefore, more scientific research institutions and drug development companies have begun using three-dimensional (3D) cells as a drug screening model. 3D cells have more similar in vivo morphological structure, drug response, gene expression, drug sensitivity, and more robust drug tolerance (Castro et al, 2021). 3D cells have shown great potential as a cell model for next-generation drug screening, which takes into account the advantages of traditional cell models in terms of high throughput, low cost, high efficiency, and animal models closer to the in vivo situation.…”

Section: Introductionmentioning

confidence: 99%

The three-dimension preclinical models for ferroptosis monitoring

Meng

Sun

Zhang

et al. 2022

Front. Bioeng. Biotechnol.

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As a new programmed cell death process, ferroptosis has shown great potential and uniqueness in experimental and treatment-resistant cancer models. Currently, the main tools for drug research targeting ferroptosis are tumor cells cultured in vitro and tumor models established in rodents. In contrast, increasing evidence indicates that reactivity may differ from modifications in mice or humans in the process of drug screening. With the blossoming of 3D culture technology, tumor organoid culture technology has gradually been utilized. Compared with traditional 2D culture and tumor tissue xenotransplantation, tumor organoids have a significantly higher success rate. They can be cultured quickly and at a lower cost, which is convenient for gene modification and large-scale drug screening. Thus, combining 3D cell culture technology, drug monitoring, and ferroptosis analysis is necessary to develop the impact of ferroptosis-related agents in tumor treatment.

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“…Therefore, the results obtained from 2D monolayer culture systems were sometimes not predictive of in vivo efficacy and did not match the results of in vivo assays [ 53 , 54 ]. However, the 3D MTS model has the potential to mimic the complex 3D organization of in vivo tumor tissue, similar to native tumor tissue [ 52 , 55 ]. Therefore, in this study, we investigated the effect and mechanism of action on IALT-induced apoptosis using 2D monolayer and 3D MTS models of HCC Hep3B cells.…”

Section: Introductionmentioning

confidence: 99%

Induction of Apoptosis by Isoalantolactone in Human Hepatocellular Carcinoma Hep3B Cells through Activation of the ROS-Dependent JNK Signaling Pathway

Kim

Lee

et al. 2021

Pharmaceutics

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Isoalantolactone (IALT) is one of the isomeric sesquiterpene lactones isolated from the roots of Inula helenium L. IALT is known to possess various biological and pharmacological activities, but its anti-cancer mechanisms are not well understood. The aim of the present study was to investigate the anti-proliferative effects of IALT in human hepatocellular carcinoma (HCC) cells and to evaluate the potential anti-cancer mechanisms. Our results demonstrated that IALT treatment concentration-dependently suppressed the cell survival of HCC Hep3B cells, which was associated with the induction of apoptosis. IALT increased the expression of death-receptor-related proteins, activated caspases, and induced Bid truncation, subsequently leading to cleavage of poly (ADP-ribose) polymerase. In addition, IALT contributed to the cytosolic release of cytochrome c by destroying mitochondrial integrity, following an increase in the Bax/Bcl-2 expression ratio. However, IALT-mediated growth inhibition and apoptosis were significantly attenuated in the presence of a pan-caspase inhibitor, suggesting that IALT induced caspase-dependent apoptosis in Hep3B cells. Moreover, IALT activated the mitogen-activated protein kinases signaling pathway, and the anti-cancer effect of IALT was significantly diminished in the presence of a potent c-Jun N-terminal kinase (JNK) inhibitor. IALT also improved the generation of intracellular reactive oxygen species (ROS), whereas the ROS inhibitor significantly abrogated IALT-induced growth reduction, apoptosis, and JNK activation. Furthermore, ROS-dependent apoptosis was revealed as a mechanism involved in the anti-cancer activity of IALT in a 3D multicellular tumor spheroid model of Hep3B cells. Taken together, our findings indicate that IALT exhibited anti-cancer activity in HCC Hep3B cells by inducing ROS-dependent activation of the JNK signaling pathway.

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Advances on colorectal cancer 3D models: The needed translational technology for nanomedicine screening

Cited by 34 publications

References 157 publications

Decellularized Colorectal Cancer Matrices as Bioactive Scaffolds for Studying Tumor-Stroma Interactions

Decellularized Colorectal Cancer Matrices as Bioactive Scaffolds for Studying Tumor-Stroma Interactions

The three-dimension preclinical models for ferroptosis monitoring

Induction of Apoptosis by Isoalantolactone in Human Hepatocellular Carcinoma Hep3B Cells through Activation of the ROS-Dependent JNK Signaling Pathway

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