Advances of Veratrum nigrum L. Steroid Alkaloids

Liu, Bo; Kou, Chengxi; Ren, Yafei; Li, Qi; Wang, Tong; Ma, Rui; Sun, Wei; Ma, Pengda

doi:10.1016/j.indcrop.2022.115946

Cited by 4 publications

(3 citation statements)

References 62 publications

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“…V. nigrum-based preparations show antihypertensive, anticancer, and antifungal effects. This species contains more than 60 steroidal alkaloids with promising medical uses, which is why it has recently attracted a lot of attention from researchers [38,39]. It is also cultivated as an ornamental plant.…”

Section: Veratrum Speciesmentioning

confidence: 99%

IPPRAS Cryobank for the Conservation of Orthodox Seeds of Rare, Endangered, Medicinal, and Ornamental Plant Species—Current Research

Nits,

Sementsova,

Osipova

et al. 2024

Plants

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Plant cryobanks play a significant role in modern science and breeding. They contribute to the recovery of lost species, the emergence of new plant varieties, and help preserve and explore the diversity of the plant world. The IPPRAS Cryobank collection is constantly supplemented with new samples, while, at the same time, the stored samples are being monitored. In order to test seed germination, seeds of Allium and Veratrum species were thawed. Rare Allium species seeds, such as A. nutans, A. schoenoprasum, and A. victorialis were stored in liquid nitrogen for 17, 19, and 30 years, respectively. Long-term cryopreservation decreased germination rates for A. nutans from 96.55 to 50.00%, for A. schoenoprasum from 72.00 to 62.75%, and for A. victorialis from 90.00 to 83.05%. Seeds of a rare medicinal species, Veratrum lobelianum, were stored in liquid nitrogen for 18 years; the seed germination rate during this storage period has been significantly decreased from 75.00 to 14.81%. V. nigrum seeds were also collected and frozen in liquid nitrogen for 3 days. Short-term cryopreservation did not result in a statistically significant change in germination rates (from 79.71 to 82.69%). The seeds of an endangered ornamental species, Cypripedium calceolus, were collected and kept frozen for 3 days. After cryopreservation, the seeds were planted on three different media, as follows: ½ MS, MS with 10% coconut milk, and BM1. On ½ MS medium, 24.98% seeds formed protocorms, while on MS medium with 10% coconut milk, this number was 10.02%, and on BM1 medium, it was 15.02%, respectively; however, after 2.5 months, all of the protocorms died. Thus, it appears that the existing protocol for seed cryopreservation of C. calceolus needs further improvement. The size, weight, and free water content (WC) of six previously cryopreserved Stipa species and three Allium species were measured. For all the Allium and Stipa species studied, we found no correlation between seed size, WC, and cryotolerance. We also found no correlation between the life form, which reflects the water requirement of the species, and cryotolerance.

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Section: Veratrum Speciesmentioning

confidence: 99%

IPPRAS Cryobank for the Conservation of Orthodox Seeds of Rare, Endangered, Medicinal, and Ornamental Plant Species—Current Research

Nits,

Sementsova,

Osipova

et al. 2024

Plants

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“…It was widely employed in the early days of metabolomics, pharmaceuticals, TCMs processing, food safety, and other analytical fields. [28][29] Given that, the aim of this study was to explore the essence of changes in chemical composition between Raw Myrrh (RM) and its processed products with different ratios of vinegar (200:0 (MQ), 200 : 5 (M40), 200 : 10 (M20), 200 : 20 (M10), and 200 : 40 (M5) (w/v)) and to elucidate the transformation mechanisms of certain changed chemical markers by using UPLC-MS/MS technology coupled with multivariate statistical analysis. This study would expand our understanding of the effects of processing on the toxicity reduction and provide a powerful guidance for rational and safety applications of TCMs in clinic.…”

Section: Introductionmentioning

confidence: 99%

Chemometrics‐based Chemical Analysis of Myrrh and Its Vinegar‐processed Products by UPLC‐MS/MS

Wang,

Li,

Rao

et al. 2024

Chemistry & Biodiversity

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Myrrh is widely used in clinical practice but accompanied by obvious toxicity. According to traditional Chinese medicines theory, processing with vinegar can effectively reduce its toxicity. However, the detoxification processing technology of Myrrh and the corresponding mechanism have been unclear. The objective of this study is to systematically analyze the variation in chemical composition of raw Myrrh and its processed products using UPLC‐Q‐TOF‐MS/MS coupled with chemometrics. A total of 75 compounds including 56 sesquiterpenoids, 2 diterpenoids, 15 triterpenoids and 2 other types were identified. Raw Myrrh and its processed products were divided into two major groups, and 14 chemical markers were selected out by principal component analysis and partial least square discriminant analysis. Additionally, the exact content of 5 representative chemical markers was determined to be significantly reduced after vinegar‐processing by UPLC‐QQQ‐MS/MS. Moreover, multivariate statistical analysis and the quantitative results comprehensively indicated that the optimized processing method was processing at a ratio of 200:5 (Myrrh:vinegar). This research provides not only a reliable foundation for the study of Myrrh, but also a scientific reference for clinical use of this herb.

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“…Among the large number of Veratrum steroid alkaloids ( Zhang et al., 2020 ), cyclopamine has been shown to have various inhibitory mechanisms against cancer, including inhibition of the hedgehog signaling pathway, improvement of tumor tissue microenvironment, inhibition of cell respiration, promotion of cell apoptosis, and reversal of tumor drug resistance ( Liu et al., 2023 ). The biosynthetic pathway of verazine, a possible metabolic precursor of cyclopamine, has been discovered in Veratrum californicum ( Augustin et al., 2015 ).…”

mentioning

confidence: 99%

Efficient heterologous biosynthesis of verazine, a metabolic precursor of the anti-cancer drug cyclopamine, in Nicotiana benthamiana

Kou,

Liu,

Yin

et al. 2024

Plant Communications

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Advances of Veratrum nigrum L. Steroid Alkaloids

Cited by 4 publications

References 62 publications

IPPRAS Cryobank for the Conservation of Orthodox Seeds of Rare, Endangered, Medicinal, and Ornamental Plant Species—Current Research

IPPRAS Cryobank for the Conservation of Orthodox Seeds of Rare, Endangered, Medicinal, and Ornamental Plant Species—Current Research

Chemometrics‐based Chemical Analysis of Myrrh and Its Vinegar‐processed Products by UPLC‐MS/MS

Efficient heterologous biosynthesis of verazine, a metabolic precursor of the anti-cancer drug cyclopamine, in Nicotiana benthamiana

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