Advances of adipose-derived mesenchymal stem cells-based biomaterial scaffolds for oral and maxillofacial tissue engineering

Liu, Tong; Xu, Jia; Pan, Xun; Ding, Zhangfan; Xie, Hao; Wang, Xiaoyi; Xie, Huixu

doi:10.1016/j.bioactmat.2021.01.015

Cited by 38 publications

(23 citation statements)

References 106 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…ADSCs have shown a high attachment rate to scaffold material, and a high differentiation capacity into the osteogenic lineage. ADSCs secret growth factors contribute to the bone tissue remodeling and angiogenesis [ 4 ]. Compared to bone marrow-derived MSCs (BMSCs), ADSCs can be obtained in a high ratio, are easily available and harvestable, and have low sensitivity to senescence [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

PCL/Col I-based magnetic nanocomposite scaffold provides an osteoinductive environment for ADSCs in osteogenic cues-free media conditions

et al. 2022

View full text Add to dashboard Cite

Background The bone tissue engineering (BTE) approach has been introduced as an alternative to conventional treatments for large non-healing bone defects. Magnetism promotes stem cells' adherence to biocompatible scaffolds toward osteoblast differentiation. Furthermore, osteogenic differentiation media are expensive and any changes in its composition affect stem cells differentiation. Moreover, media growth factors possess a short half-life resulting in the rapid loss of their functions in vivo. With the above in mind, we fabricated a multilayered nanocomposite scaffold containing the wild type of Type I collagen (Col I) with endogenous magnetic property to promote osteogenesis in rat ADSCs with the minimum requirement of osteogenic differentiation medium. Methods Fe3O4 NPs were synthesized by co-precipitation method and characterized using SEM, VSM, and FTIR. Then, a PCL/Col I nanocomposite scaffold entrapping Fe3O4 NPs was fabricated by electrospinning and characterized using SEM, TEM, AFM, VSM, Contact Angle, tensile stretching, and FTIR. ADSCs were isolated from rat adipose tissue and identified by flow cytometry. ADSCs were loaded onto PCL/Col I and PCL/Col I/Fe3O4-scaffolds for 1–3 weeks with/without osteogenic media conditions. The cell viability, cell adhesion, and osteogenic differentiation were evaluated using MTT assay, SEM, DAPI staining, ALP/ARS staining, RT-PCR, and western blotting, respectively. Results SEM, VSM, and FTIR results indicated that Fe3O4 was synthesized in nano-sized (15–30 nm) particles with spherical-shaped morphology and superparamagnetic properties with approved chemical structure as FTIR revealed. According to SEM images, the fabricated magnetic scaffolds consisted of nanofiber (500–700 nm). TEM images have shown the Fe3O4 NPs entrapped in the scaffold's fiber without bead formation. FTIR spectra analysis confirmed the maintenance of the natural structure of Col I, PCL, and Fe3O4 upon electrospinning. AFM data have shown that MNPs incorporation introduced stripe-like topography to nanofibers, while the depth of the grooves has decreased from 800 to 500 nm. Flow cytometry confirmed the phenotype of ADSCs according to their surface markers (i.e., CD29 and CD105). Additionally, Fe3O4 NP improved nanocomposite scaffold strength, wettability, porosity, biocompatibility and also facilitates the ALP activity, calcium-mineralization. Finally, magnetic nanocomposite scaffolds upregulated osteogenic-related genes or proteins’ expression (e.g., Col I, Runx2, OCN, ON, BMP2) in seeded ADSCs with/without osteo-differentiation media conditions. Conclusions Together, these results indicate that Fe3O4 NPs within the natural structure of Col I increase osteogenic differentiation in osteogenic cues-free media conditions. This effect could be translated in vivo toward bone defects healing. These findings support the use of natural ECM materials alongside magnetic particles as composite scaffolds to achieve their full therapeutic potential in BTE treatments. Graphical Abstract

show abstract

Section: Introductionmentioning

confidence: 99%

“…ADSCs secret growth factors contribute to the bone tissue remodeling and angiogenesis [ 4 ]. Compared to bone marrow-derived MSCs (BMSCs), ADSCs can be obtained in a high ratio, are easily available and harvestable, and have low sensitivity to senescence [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

PCL/Col I-based magnetic nanocomposite scaffold provides an osteoinductive environment for ADSCs in osteogenic cues-free media conditions

et al. 2022

View full text Add to dashboard Cite

show abstract

“…A more recent approach for the development of advanced nanomaterials with bioactive properties in the field of tissue engineering comprises the seeding of stem cells on suitable scaffolds [148][149][150]. Stem cells represent a special type of cell that can mostly be isolated from the placenta, adipose tissue, bone marrow, and dental pulp and have the ability to grow and differentiate into numerous classes of specialized cell lines.…”

Section: Advances and Challenges In Tissue Engineering Platformsmentioning

confidence: 99%

Graphene Oxide–Protein-Based Scaffolds for Tissue Engineering: Recent Advances and Applications

et al. 2022

View full text Add to dashboard Cite

The field of tissue engineering is constantly evolving as it aims to develop bioengineered and functional tissues and organs for repair or replacement. Due to their large surface area and ability to interact with proteins and peptides, graphene oxides offer valuable physiochemical and biological features for biomedical applications and have been successfully employed for optimizing scaffold architectures for a wide range of organs, from the skin to cardiac tissue. This review critically focuses on opportunities to employ protein–graphene oxide structures either as nanocomposites or as biocomplexes and highlights the effects of carbonaceous nanostructures on protein conformation and structural stability for applications in tissue engineering and regenerative medicine. Herein, recent applications and the biological activity of nanocomposite bioconjugates are analyzed with respect to cell viability and proliferation, along with the ability of these constructs to sustain the formation of new and functional tissue. Novel strategies and approaches based on stem cell therapy, as well as the involvement of the extracellular matrix in the design of smart nanoplatforms, are discussed.

show abstract

“…Tang et al reported that MSCs and iPSCs had good viability and osteogenic differentiation, when seeded onto CPC scaffold and could promote bone regeneration in craniofacial defects [192]. In recent years, AdSCsbased biomaterial scaffolds were shown to cover the needs of oral and maxillofacial tissue engineering because of their superior performance [193].…”

Section: Head and Neck Surgeriesmentioning

confidence: 99%

Achievements and Challenges in Transplantation of Mesenchymal Stem Cells in Otorhinolaryngology

Kaboodkhani

Mehrabani

Karimi‐Busheri

2021

JCM

View full text Add to dashboard Cite

Otorhinolaryngology enrolls head and neck surgery in various tissues such as ear, nose, and throat (ENT) that govern different activities such as hearing, breathing, smelling, production of vocal sounds, the balance, deglutition, facial animation, air filtration and humidification, and articulation during speech, while absence of these functions can lead to high morbidity and even mortality. Conventional therapies for head and neck damaged tissues include grafts, transplants, and artificial materials, but grafts have limited availability and cause morbidity in the donor site. To improve these limitations, regenerative medicine, as a novel and rapidly growing field, has opened a new therapeutic window in otorhinolaryngology by using cell transplantation to target the healing and replacement of injured tissues. There is a high risk of rejection and tumor formation for transplantation of embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs); mesenchymal stem cells (MSCs) lack these drawbacks. They have easy expansion and antiapoptotic properties with a wide range of healing and aesthetic functions that make them a novel candidate in otorhinolaryngology for craniofacial defects and diseases and hold immense promise for bone tissue healing; even the tissue sources and types of MSCs, the method of cell introduction and their preparation quality can influence the final outcome in the injured tissue. In this review, we demonstrated the anti-inflammatory and immunomodulatory properties of MSCs, from different sources, to be safely used for cell-based therapies in otorhinolaryngology, while their achievements and challenges have been described too.

show abstract

Advances of adipose-derived mesenchymal stem cells-based biomaterial scaffolds for oral and maxillofacial tissue engineering

Cited by 38 publications

References 106 publications

PCL/Col I-based magnetic nanocomposite scaffold provides an osteoinductive environment for ADSCs in osteogenic cues-free media conditions

PCL/Col I-based magnetic nanocomposite scaffold provides an osteoinductive environment for ADSCs in osteogenic cues-free media conditions

Graphene Oxide–Protein-Based Scaffolds for Tissue Engineering: Recent Advances and Applications

Achievements and Challenges in Transplantation of Mesenchymal Stem Cells in Otorhinolaryngology

Contact Info

Product

Resources

About