Advances, limitations, and prospects of biosensing technology for detecting phytopathogenic bacteria

Ali, Qurban; Zheng, Hongxia; Rao, Muhammad Junaid; Mohsin, Ali; Hussain, Amjad; Saleem, Muhammad Hamzah; Nehela, Yasser; Sohail, Muhammad Aamir; Ahmed, Agha Mushtaque; Kubar, Kashif Ali; Ali, Shafaqat; Usman, Kamal; Manghwar, Hakim; Zhou, Lei

doi:10.1016/j.chemosphere.2022.133773

Cited by 45 publications

(31 citation statements)

References 179 publications

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“…Plant pathogens, such as fungi, bacteria, viruses, and nematodes, cause different plant diseases, resulting in yield loss in crops around the globe [ 1 , 2 ]. Plant parasitic nematodes are currently serious pests, contributing to USD 157 billion in annual agricultural losses worldwide [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Toxicity of Bacillus thuringiensis Strains Derived from the Novel Crystal Protein Cry31Aa with High Nematicidal Activity against Rice Parasitic Nematode Aphelenchoides besseyi

Zhao

Ali

Wang

et al. 2022

IJMS

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The plant parasitic nematode, Aphelenchoides besseyi, is a serious pest causing severe damage to various crop plants and vegetables. The Bacillus thuringiensis (Bt) strains, GBAC46 and NMTD81, and the biological strain, FZB42, showed higher nematicidal activity against A. besseyi, by up to 88.80, 82.65, and 75.87%, respectively, in a 96-well plate experiment. We screened the whole genomes of the selected strains by protein-nucleic acid alignment. It was found that the Bt strain GBAC46 showed three novel crystal proteins, namely, Cry31Aa, Cry73Aa, and Cry40ORF, which likely provide for the safe control of nematodes. The Cry31Aa protein was composed of 802 amino acids with a molecular weight of 90.257 kDa and contained a conserved delta-endotoxin insecticidal domain. The Cry31Aa exhibited significant nematicidal activity against A. besseyi with a lethal concentration (LC50) value of 131.80 μg/mL. Furthermore, the results of in vitro experiments (i.e., rhodamine and propidium iodide (PI) experiments) revealed that the Cry31Aa protein was taken up by A. besseyi, which caused damage to the nematode’s intestinal cell membrane, indicating that the Cry31Aa produced a pore-formation toxin. In pot experiments, the selected strains GBAC46, NMTD81, and FZB42 significantly reduced the lesions on leaves by up to 33.56%, 45.66, and 30.34% and also enhanced physiological growth parameters such as root length (65.10, 50.65, and 55.60%), shoot length (68.10, 55.60, and 59.45%), and plant fresh weight (60.71, 56.45, and 55.65%), respectively. The number of nematodes obtained from the plants treated with the selected strains (i.e., GBAC46, NMTD81, and FZB42) and A. besseyi was significantly reduced, with 0.56, 0.83., 1.11, and 5.04 seedling mL−1 nematodes were achieved, respectively. Moreover, the qRT-PCR analysis showed that the defense-related genes were upregulated, and the activity of hydrogen peroxide (H2O2) increased while malondialdehyde (MDA) decreased in rice leaves compared to the control. Therefore, it was concluded that the Bt strains GBAC46 and NMTD81 can promote rice growth, induce high expression of rice defense-related genes, and activate systemic resistance in rice. More importantly, the application of the novel Cry31Aa protein has high potential for the efficient and safe prevention and green control of plant parasitic nematodes.

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Section: Introductionmentioning

confidence: 99%

Toxicity of Bacillus thuringiensis Strains Derived from the Novel Crystal Protein Cry31Aa with High Nematicidal Activity against Rice Parasitic Nematode Aphelenchoides besseyi

Zhao

Ali

Wang

et al. 2022

IJMS

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“…Moreover, the evolution of diagnostic assays has been varied from conventional culture-based techniques to immunological molecular detection, and recently biosensors. Biosensing approaches (such as colorimetric, fluorescent, electrochemical, and others) require a small sample volume, thereby decreasing the experimental setup, analytical time, and expenses [ 114 , 115 , 116 ]. The immobilization of target pathogens on the surface of the bioreceptor can be strengthened by nanomaterials [ 117 , 118 ].…”

Section: Emerging Pathogen-detection Techniquesmentioning

confidence: 99%

Current Scenario of Pathogen Detection Techniques in Agro-Food Sector

Nehra

Kumar

et al. 2022

Biosensors

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Over the past-decade, agricultural products (such as vegetables and fruits) have been reported as the major vehicles for foodborne diseases, which are limiting food resources. The spread of infectious diseases due to foodborne pathogens poses a global threat to human health and the economy. The accurate and timely detection of infectious disease and of causative pathogens is crucial in the prevention and treatment of disease. Negligence in the detection of pathogenic substances can be catastrophic and lead to a pandemic. Despite the revolution in health diagnostics, much attention has been paid to the agro-food sector regarding the detection of food contaminants (such as pathogens). The conventional analytical techniques for pathogen detection are reliable and still in operation. However, laborious procedures and time-consuming detection via these approaches emphasize the need for simple, easy-to-use, and affordable detection techniques. The rapid detection of pathogens from food is essential to avoid the morbidity and mortality originating from the suboptimal nature of empiric pathogen treatment. This review critically discusses both the conventional and emerging bio-molecular approaches for pathogen detection in agro-food.

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“…According to (Zubrod et al 2019), the world population increases to approximately 97 million people each year. Around 50,000 plant pathogen species, 9000 insect and mite species, and 8000 weed species cause agricultural loss worldwide, with most of the damage happening in the United States (Ali et al 2022b). Plant infections are estimated to have caused a 13% loss in agricultural production, insect pests a 14% loss in crop production, and weeds a 13% loss in crop production.…”

Section: The Use Of Pesticides As a Source Of Heavy Metalsmentioning

confidence: 99%

Principles and Applicability of Integrated Remediation Strategies for Heavy Metal Removal/Recovery from Contaminated Environments

Alsafran

Saleem

Jabri

et al. 2022

J Plant Growth Regul

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Contamination of agricultural soils with heavy metals present lethal consequences in terms of diverse ecological and environmental problems that entail entry of metal in food chain, soil deterioration, plant growth suppression, yield reduction and alteration in microbial community. Metal polluted soils have become a major concern for scientists around the globe. In more recent times, armed with new knowledge and understanding, removal of heavy metals using different applications has emerged as a solution for waste treatment and contaminant remediation in water and soil. However, the description of metal toxicity to the plants and its removal and degradation from the soil is limited. There are a number of reports in the literature where PGP bacterial inoculation and various chelating agents improves metal accumulation and it’s detoxification in different plant parts without influencing plant growth. Therefore, there is a need to select some useful chemicals which possess the potential to improve plant growth as well as expedite the phytoremediation of metals. In this review, we have discussed the mechanisms possessed by different chelating agents to promote plant growth and phytoremediation of metals. We anticipate that this analysis of interconnected systems will lead to the discovery of new research fields.

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Advances, limitations, and prospects of biosensing technology for detecting phytopathogenic bacteria

Cited by 45 publications

References 179 publications

Toxicity of Bacillus thuringiensis Strains Derived from the Novel Crystal Protein Cry31Aa with High Nematicidal Activity against Rice Parasitic Nematode Aphelenchoides besseyi

Toxicity of Bacillus thuringiensis Strains Derived from the Novel Crystal Protein Cry31Aa with High Nematicidal Activity against Rice Parasitic Nematode Aphelenchoides besseyi

Current Scenario of Pathogen Detection Techniques in Agro-Food Sector

Principles and Applicability of Integrated Remediation Strategies for Heavy Metal Removal/Recovery from Contaminated Environments

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