Advances in Zinc Finger Nuclease and Its Applications

Tang, Li-Meng; Zhou, Changyong; Guo, Zifen; Xiao, Li; Chüeh, Anderly C.

doi:10.1166/gge.2015.1010

Cited by 5 publications

(6 citation statements)

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“…97 For instance regarding ZFNs, these include increasing the ZF motif numbers, forming obligatory heterodimer through inserting amino acid substitutions at the dimer interface of the cleavage domain or using ZF nickases. 12,98 Similarly for TALENs, optimizing the number and/or the nature of the TALE modules may increase the specificity. 97 However, the use of TALENmediated genome engineering in clinic is also restricted due to their large size (nearly 3 kb is the typical size of a TALEN encoding cDNA).…”

Section: Genome Editing Challengesmentioning

confidence: 99%

“…Beside optimization of delivery vehicles, a number of approaches have been used to improve the specificity of these tools 97 . For instance regarding ZFNs, these include increasing the ZF motif numbers, forming obligatory heterodimer through inserting amino acid substitutions at the dimer interface of the cleavage domain or using ZF nickases 12,98 . Similarly for TALENs, optimizing the number and/or the nature of the TALE modules may increase the specificity 97 .…”

Section: Clinical Studiesmentioning

confidence: 99%

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Gene editing technology for improving life quality: A dream coming true?

et al. 2020

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The fact that monogenic diseases are related to mutations in one specific gene, make gene correction one of the promising strategies in the future to treat genetic diseases or alleviate their symptoms. From this perspective, and along with recent advances in technology, genome editing tools have gained momentum and developed fast. In fact, clustered regularly interspaced short palindromic repeats-associated protein 9 (CRISPR/Cas9), transcription activator-like effector nucleases (TALENs), and zincfinger nucleases (ZFNs) are regarded as novel technologies which are able to correct a number of genetic aberrations in vitro and in vivo. The number of ongoing clinical trials employing these tools has been increased showing the encouraging outcomes of these tools. However, there are still some major challenges with respect to the safety profile and directed delivery of them. In this paper, we provided updated information regarding the history, nature, methods of delivery, and application of the above-mentioned gene editing tools along with the meganucleases (an older similar tool) based on published in vitro and in vivo studies and introduced clinical trials which employed these technologies.

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Section: Genome Editing Challengesmentioning

confidence: 99%

Section: Clinical Studiesmentioning

confidence: 99%

Gene editing technology for improving life quality: A dream coming true?

et al. 2020

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“…The principle is that different zinc fingers identify different sets of nucleotide triplets. This hybrid protein consists of specific DNA-binding domains that fuse with the endonuclease Fok I, created to target specific genome sequences [ 26 , 27 , 28 , 29 ]. Zinc finger proteins (ZFPs) have a unique ability to recognize and bind to specific DNA sequences, and ZFN enzymes can cut the DNA in the targeted sequences [ 29 ].…”

Section: Introductionmentioning

confidence: 99%

“…This hybrid protein consists of specific DNA-binding domains that fuse with the endonuclease Fok I, created to target specific genome sequences [ 26 , 27 , 28 , 29 ]. Zinc finger proteins (ZFPs) have a unique ability to recognize and bind to specific DNA sequences, and ZFN enzymes can cut the DNA in the targeted sequences [ 29 ]. In addition, ZFN can create a DNA double-strand break (DSB) at preselected sites [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

“…The significant concern for ZFNs is off-target cleavage, contrary to many natural endonucleases [ 30 ]. ZFNs and ZFPs are classified in three major subtypes (C2H2, C4 and C6), in which C2H2 is, due to its simplicity, the most broadly used in engineered ZFNs [ 29 , 31 ]. The major advantage of this technique compared to standard gene therapy is the potential to conserve temporal and tissue-specific gene expression [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

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Review: Recent Applications of Gene Editing in Fish Species and Aquatic Medicine

Gutási

Hammer

El-Matbouli

et al. 2023

Animals

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Gene editing and gene silencing techniques have the potential to revolutionize our knowledge of biology and diseases of fish and other aquatic animals. By using such techniques, it is feasible to change the phenotype and modify cells, tissues and organs of animals in order to cure abnormalities and dysfunctions in the organisms. Gene editing is currently experimental in wide fields of aquaculture, including growth, controlled reproduction, sterility and disease resistance. Zink finger nucleases, TALENs and CRISPR/Cas9 targeted cleavage of the DNA induce favorable changes to site-specific locations. Moreover, gene silencing can be used to inhibit the translation of RNA, namely, to regulate gene expression. This methodology is widely used by researchers to investigate genes involved in different disorders. It is a promising tool in biotechnology and in medicine for investigating gene function and diseases. The production of food fish has increased markedly, making fish and seafood globally more popular. Consequently, the incidence of associated problems and disease outbreaks has also increased. A greater investment in new technologies is therefore needed to overcome such problems in this industry. To put it concisely, the modification of genomic DNA and gene silencing can comprehensively influence aquatic animal medicine in the future. On the ethical side, these precise genetic modifications make it more complicated to recognize genetically modified organisms in nature and can cause several side effects through created mutations. The aim of this review is to summarize the current state of applications of gene modifications and genome editing in fish medicine.

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