Advances in yeast preservation: physiological aspects for cell perpetuation

Câmara, Antonio A.; Sant’Ana, Anderson S.

doi:10.1016/j.cofs.2020.10.019

Cited by 19 publications

(9 citation statements)

References 61 publications

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“…During desiccation, yeast cells face mechanical, structural, and oxidative challenges, including intracellular crowding, plasma membrane lysis, and permeabilization. To survive these stresses, yeasts have developed many endogenous protective mechanisms, including a unique elastic cell wall, the accumulation of intracellular glycerol and trehalose, the induction of stress proteins, and biochemical antioxidative systems [ 2 , 15 ].…”

Section: Discussionmentioning

confidence: 99%

Changes in Energy Status of Saccharomyces cerevisiae Cells during Dehydration and Rehydration

et al. 2021

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Anhydrobiosis is the state of life when cells are exposed to waterless conditions and gradually cease their metabolism. In this study, we determined the sequence of events in Saccharomyces cerevisiae energy metabolism during processes of dehydration and rehydration. The intensities of respiration and acidification of the medium, the amounts of phenyldicarbaundecaborane (PCB−) bound to yeast membranes, and the capabilities of cells to accumulate K+ were assayed using an electrochemical monitoring system, and the intracellular content of ATP was measured using a bioluminescence assay. Mesophilic, semi-resistant to desiccation S. cerevisiae strain 14 and thermotolerant, very resistant to desiccation S. cerevisiae strain 77 cells were compared. After 22 h of drying, it was possible to restore the respiration activity of very resistant to desiccation strain 77 cells, especially when glucose was available. PCB− binding also indicated considerably higher metabolic activity of dehydrated S. cerevisiae strain 77 cells. Electrochemical K+ content and medium acidification assays indicated that permeabilization of the plasma membrane in cells of both strains started almost simultaneously, after 8–10 h of desiccation, but semi-resistant strain 14 cells maintained the K+ gradient for longer and more strongly acidified the medium. For both cells, the fast rehydration in water was less efficient compared to reactivation in the growth medium, indicating the need for nutrients for the recovery. Higher viability of strain 77 cells after rehydration could be due to the higher stability of their mitochondria.

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Section: Discussionmentioning

confidence: 99%

Changes in Energy Status of Saccharomyces cerevisiae Cells during Dehydration and Rehydration

et al. 2021

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show abstract

“…During desiccation, yeast cells face mechanical, structural, and oxidative challenges, including intracellular crowding, plasma membrane lysis, and permeabilization. To survive these stresses, the yeasts have developed many endogenous protective mechanisms, including a unique elastic cell wall, the accumulation of intracellular glycerol and trehalose, the induction of stress proteins and biochemical antioxidative systems [2,20].…”

Section: Discussionmentioning

confidence: 99%

“…While losing the water content, yeasts synthesize the endogenous compounds, such as glycerol and trehalose [2]. A rapid change in the cellular energy metabolism reflects the higher energy demands required for surviving solutes synthesis against osmotic shock [19,20]. After ATP depletion, Marini et al [21] observed significant cytosolic compaction and extensive cytoplasmic reorganization, as well as the emergence of distinct membrane-bound and membraneless organelles.…”

Section: Discussionmentioning

confidence: 99%

Changes in Energy Status of Saccharomyces cerevisiae Cells During Dehydration and Rehydration

Kuliešienė¹,

Žūkienė²,

Khroustalyova³

et al. 2021

Preprint

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Anhydrobiosis is the state of life when cells get into waterless conditions and gradually cease their metabolism. In this study, we determined the sequence of events in Saccharomyces cerevisiae energy metabolism during processes of dehydration and rehydration. The intensities of respiration and acidification of the medium, the amounts of Phenyldicarbaundecaborane (PCB-) bound to yeast membranes, and the capabilities of cells to accumulate K+ were assayed using electrochemical monitoring system, and intracellular content of ATP was measured using bioluminescence assay. Mesophilic, semi-resistant to desiccation S. cerevisiae strain 14 and thermotolerant, very resistant to desiccation S. cerevisiae strain 77 cells were compared. After 22 h of drying it was possible to restore the respiration activity of very resistant to desiccation strain 77 cells, especially when glucose was available. PCB- binding also indicated considerably higher metabolic activity of dehydrated S. cerevisiae strain 77 cells. Electrochemical K+ content and medium acidification assays indicated that permeabilization of the plasma membrane in cells of both strains started almost simultaneously, after 8-10 h of desiccation, but semi-resistant strain 14 cells were longer keeping K+ gradient and stronger acidifying the medium. For both cells, the fast rehydration in water was less efficient compared to reactivation in the growth medium, indicating the need for nutrients for the recovery. Higher viability of strain 77 cells after rehydration could be due to the higher stability of their mitochondria.

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“…This state-of-the-art was analogous to the development of anhydrobiotic studies, regarding microbial anhydrobiosis, it has been focused mainly on yeast (one must remember, though, that this situation is highly influenced by historic and commercial importance of Saccharomyces cerevisiae), cf. [5,16,28,29,[43][44][45][46][47][48][49][50][51]. Regarding lipids and lipid domains, it is known, for example, that dehydration in yeast cells causes the decrease in spacing between membrane phospholipids and ordering of the hydrocarbon chains and additionally may disorder the hydrophobic chains of the lipid, as a result of the division of amphiphilic molecules between the aqueous cytoplasm and the lipid phase of membranes during drying of [44].…”

Section: Lipid Characteristics Of the Cytoplasmic Membranementioning

confidence: 99%

Introduction to Bacterial Anhydrobiosis: A General Perspective and the Mechanisms of Desiccation-Associated Damage

Grzyb

Skłodowska

2022

Microorganisms

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Anhydrobiosis is the ability of selected organisms to lose almost all water and enter a state of reversible ametabolism. Such an organism dries up to a state of equilibrium with dry air. Unless special protective mechanisms exist, desiccation leads to damage, mainly to proteins, nucleic acids, and membrane lipids. A short historical outline of research on extreme dehydration of living organisms and the current state of research are presented. Terminological issues are outlined. The role of water in the cell and the mechanisms of damage occurring in the cell under the desiccation stress are briefly discussed. Particular attention was paid to damage to proteins, nucleic acids, and membrane lipids. Understanding the nature of the changes and damage associated with desiccation is essential for the study of desiccation-tolerance mechanisms and application research. Difficulties related to the definition of life and the limits of life in the scientific discussion, caused by the phenomenon of anhydrobiosis, were also indicated.

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Advances in yeast preservation: physiological aspects for cell perpetuation

Cited by 19 publications

References 61 publications

Changes in Energy Status of Saccharomyces cerevisiae Cells during Dehydration and Rehydration

Changes in Energy Status of Saccharomyces cerevisiae Cells during Dehydration and Rehydration

Changes in Energy Status of Saccharomyces cerevisiae Cells During Dehydration and Rehydration

Introduction to Bacterial Anhydrobiosis: A General Perspective and the Mechanisms of Desiccation-Associated Damage

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