Advances in Wound-Healing Assays for Probing Collective Cell Migration

Riahi, Reza; Yang, Yongliang; Zhang, Donna D.; Wong, Pak Kin

doi:10.1177/2211068211426550

Cited by 135 publications

(151 citation statements)

References 43 publications

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“…98 In addition, physical stimulation with a mechanical probe may also be applied to create local mechanical stress and injury to individual cells in the tissue. [99][100][101] A mechanical probe with comb-drive (capacitive) force measurement, for instance, has been used to study mechanical stimulation-induced intercellular calcium communication in microengineered endothelial networks. 102,103 Endothelial cells collectively regulated their calcium levels against a large range of probing forces and repeated stimulations.…”

Section: Mechanical Perturbationmentioning

confidence: 99%

Advances in Techniques for Probing Mechanoregulation of Tissue Morphogenesis

et al. 2015

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Section: Mechanical Perturbationmentioning

confidence: 99%

Advances in Techniques for Probing Mechanoregulation of Tissue Morphogenesis

et al. 2015

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“…Furthermore, the most commonly used method of mechanical scraping to create the cell-free region often results in non-uniform wounds and variable levels of cell injury, meaning experiments can be difficult to replicate. 35 Finally, wound healing assays tell us only about trophoblast migration, and not about the capacity of trophoblasts to invade through extracellular matrices or into the decidua. Thus, to relate these findings to the in vivo situation such experiments should be backed up by additional studies of trophoblast invasion.…”

Section: Functional Assays To Study Trophoblast Migrationmentioning

confidence: 99%

“…The simplest of these is wound healing assays, [32][33][34] where cell lines are grown to near-confluence, and then a 'wound' in the monolayer is created by mechanical or enzymatic means. 35 The migration of cells into this cell-free region is then monitored either by time-lapse microscopy, or over a set time-course, and the proportion of the wound filled with cells quantified by digital image analysis. Thus, the longer a wound takes to fill, the less migratory the cells in those culture conditions are considered to be.…”

Section: Functional Assays To Study Trophoblast Migrationmentioning

confidence: 99%

Quantifying trophoblast migration: In vitro approaches to address in vivo situations

James

Tun

Clark

2016

Cell Adhesion & Migration

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When trophoblasts migrate and invade in vivo, they do so by interacting with a range of other cell types, extracellular matrix proteins, chemotactic factors and physical forces such as fluid shear stress. These factors combine to influence overall trophoblast migration and invasion into the decidua, which in turn determines the success of spiral artery remodeling, and pregnancy itself. Our understanding of these important but complex processes is limited by the simplified conditions in which we often study cell migration in vitro, and many discrepancies are observed between different in vitro models in the literature. On top of these experimental considerations, the migration of individual trophoblasts can vary widely. While time-lapse microscopy provides a wealth of information on trophoblast migration, manual tracking of individual cell migration is a time consuming task that ultimately restricts the numbers of cells quantified, and thus the ability to extract meaningful information from the data. However, the development of automated imaging algorithms is likely to aid our ability to accurately interpret trophoblast migration in vitro, and better allow us to relate these observations to in vivo scenarios. This commentary discusses the advantages and disadvantages of techniques commonly used to quantify trophoblast migration and invasion, both from a cell biology and a mathematical perspective, and examines how such techniques could be improved to help us relate trophoblast migration more accurately to in vivo function in the future.

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“…The availability of this wound-repair model opens new possibilities for identifying the endogenous signaling cues from matrix proteins, cytokines and chemokines that regulate the repair process. In addition, the model is ideal for examining how an epithelium is able to move as a collective sheet to reepithelialize the wound area 2,3 , and for determining the lineage of mesenchymal leader cells at the wound edge that function in directing the collective migration of the injured epithelium 4 . This model also provides a platform with which to identify therapeutics that could promote effective wound healing and prevent aberrant wound repair 5 .…”

Section: Introductionmentioning

confidence: 99%

Establishment of a Clinically Relevant <em>Ex Vivo</em> Mock Cataract Surgery Model for Investigating Epithelial Wound Repair in a Native Microenvironment

Walker

Bleaken

Wolff

et al. 2015

JoVE

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The major impediment to understanding how an epithelial tissue executes wound repair is the limited availability of models in which it is possible to follow and manipulate the wound response ex vivo in an environment that closely mimics that of epithelial tissue injury in vivo. This issue was addressed by creating a clinically relevant epithelial ex vivo injury-repair model based on cataract surgery. In this culture model, the response of the lens epithelium to wounding can be followed live in the cells' native microenvironment, and the molecular mediators of wound repair easily manipulated during the repair process. To prepare the cultures, lenses are removed from the eye and a small incision is made in the anterior of the lens from which the inner mass of lens fiber cells is removed. This procedure creates a circular wound on the posterior lens capsule, the thick basement membrane that surrounds the lens. This wound area where the fiber cells were attached is located just adjacent to a continuous monolayer of lens epithelial cells that remains linked to the lens capsule during the surgical procedure. The wounded epithelium, the cell type from which fiber cells are derived during development, responds to the injury of fiber cell removal by moving collectively across the wound area, led by a population of vimentin-rich repair cells whose mesenchymal progenitors are endogenous to the lens 1 . These properties are typical of a normal epithelial wound healing response. In this model, as in vivo, wound repair is dependent on signals supplied by the endogenous environment that is uniquely maintained in this ex vivo culture system, providing an ideal opportunity for discovery of the mechanisms that regulate repair of an epithelium following wounding. Video LinkThe video component of this article can be found at

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Advances in Wound-Healing Assays for Probing Collective Cell Migration

Cited by 135 publications

References 43 publications

Advances in Techniques for Probing Mechanoregulation of Tissue Morphogenesis

Advances in Techniques for Probing Mechanoregulation of Tissue Morphogenesis

Quantifying trophoblast migration: In vitro approaches to address in vivo situations

Establishment of a Clinically Relevant <em>Ex Vivo</em> Mock Cataract Surgery Model for Investigating Epithelial Wound Repair in a Native Microenvironment

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