Advances in vaccination against avian pathogenic Escherichia coli respiratory disease: Potentials and limitations

Ghunaim, Haitham; Abu-Madi, Marawan; Kariyawasam, Subhashinie

doi:10.1016/j.vetmic.2014.04.019

Cited by 119 publications

(107 citation statements)

References 55 publications

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“…The increase in antibiotic resistant APEC [7], the limited effect of current vaccine [8], and the ability of APEC to establish persistent infections through the formation of biofilms [2] emphasize the need for alternate control strategies for APEC. A great effort has been made to develop anti-pathogenic drugs by reducing the bacterial virulence through QSIs, which proves to be an intriguing approach for antimicrobial chemotherapy [31].…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Currently, APEC infections in poultry are controlled by a commercially available modified-live vaccine (Poulvac E. coli) [8]. However, the vaccination does not provide complete protection against all APEC serotypes, and high mortality in vaccinated broilers due to virulent APEC infections has been reported [8]. In addition, antimicrobials such as cephalosporins, tetracyclines, and quinolones that are used for treating APEC infections have limited effects due to the emergence of antimicrobial resistant strains [7].…”

Section: Introductionmentioning

confidence: 99%

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Novel small molecule modulators of quorum sensing in avian pathogenic Escherichia coli (APEC)

et al. 2018

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Colibacillosis caused by avian pathogenic E. coli (APEC), is an economically important bacterial disease of poultry. APEC are a subgroup of extra intestinal pathogenic E. coli (ExPEC) and poultry are considered potential sources of foodborne ExPEC to humans. Currently, APEC infections in poultry are controlled by antibiotics and/or vaccination; however, their effect is limited due to emergence of antibiotic resistant strains and infections with heterologous serotypes. Therefore, novel approaches are needed. Here, using the bioluminescent quorum sensing (QS) autoinducer 2 (AI-2) indicator Vibrio harveyi BB170, we screened the cell free culture supernatant of APEC O78 prepared from cultures grown in the presence of 4,182 small molecules (SMs; 100 μM). A total of 69 SMs inhibited > 75% of APEC O78 AI-2 activity in the indicator bacteria. Ten SMs that showed highest AI-2 inhibition were selected for further studies. Most of these SMs inhibited the AI-2 activity of other APEC serotypes and significantly reduced APEC O78 biofilm formation and motility. Most compounds showed minimal toxicity on human intestinal cells (Caco-2), chicken macrophage (HD-11), and chicken and sheep red blood cells, and reduced APEC survival in HD-11 and THP-1 macrophages. The SMs induced no or minimal toxicity and conferred protection against APEC in wax moth larval model. SMs affected the expression of APEC O78 QS, virulence, biofilm and motility associated genes providing insight on their potential mode(s) of action. Further testing in chickens will facilitate development of these SMs as novel therapeutics to control APEC in poultry and thereby also reduce zoonotic transmission. ARTICLE HISTORY

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Novel small molecule modulators of quorum sensing in avian pathogenic Escherichia coli (APEC)

et al. 2018

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“…Vaccination is considered the most effective and economical means of controlling infectious diseases. Many vaccine candidates have been developed against APEC infection in chickens, including inactivated, live attenuated and subunits vaccines [9]. Inactivated vaccines were initially developed by killing the live whole-bacteria, which have not been widely used because of their low protective e cacy.…”

Section: Introductionmentioning

confidence: 99%

Exploiting bacterial outer membrane vesicles as a cross-protective vaccine candidate against avian pathogenic Escherichia coli (APEC)

Zhao

et al. 2020

Preprint

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Background: The well-known fact that avian pathogenic Escherichia coli (APEC) is harder to prevent due to its numerous serogroups has promoted the development of biological immunostimulatory materials as new vaccine candidates in poultry farms. Bacterial outer membrane vesicles (OMVs), known as spherical nanovesicles enriched with various immunostimulants, are naturally secreted by Gram-negative bacteria, and have gained much attention for developing effective vaccine candidates. Recent report has demonstrated that OMVs of APEC O78 can induce protective immunity in chickens. Here, a novel multi-serogroup OMVs (MOMVs) vaccine was developed to achieve cross-protection against APEC infection in broiler chickens.Results: In this study, OMVs produced by three APEC strains were isolated, purified and prepared into MOMVs by mixing these three OMVs. By using SDS-PAGE and LC-MS/MS, 159 proteins were identified in MOMVs and the subcellular location and biological functions of 20 most abundant proteins were analyzed. The immunogenicity of MOMVs was evaluated, and the results showed that MOMVs could elicit innate immune responses, including internalization by chicken macrophage and production of immunomodulatory cytokines. Vaccination with MOMVs induced specific broad-spectrum antibodies as well as Th1 and Th17 immune responses. The animal experiment has confirmed that immunization with an appropriate dose of MOMVs could not cause any adverse effect and was able to reduce bacteria loads and pro-inflammatory cytokines production, thus providing eﬀective cross-protection against lethal infections induced by multi-serogroup APEC strains in chickens. Further experiments indicated that, although vesicular proteins were able to induce stronger protective efficiency than lipopolysaccharide, both vesicular proteins and lipopolysaccharide are crucial in MOMVs-mediated protection. Conclusions: The multi-serogroup nanovesicles produced by APEC strains will open up a new way for the development of next generation vaccines with low toxicity and broad protection in the treatment and control of APEC infection.

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“…E. coli can produce the enterotoxin that causes colibacillosis, which brings untold damage for the poultry production [2, 3]. E. coli whose serotypes are O1, O2, O78, O15 and O55 had been found to be related to colibacillosis in chickens [4, 5], which may undermine immune function to predispose host animals to the colonization of the pathogens, placing the threat to health and food safety. Although antibiotic therapy is effective for colibacillosis, there are increasing restriction and ban to limit the use of antibiotic to poultry.…”

Section: Introductionmentioning

confidence: 99%

Effects of Lactobacillus plantarum 15-1 and fructooligosaccharide on the response of broilers to pathogenic Escherichia coli O₇₈ challenge

Ding

Wang

Yan

et al. 2019

Preprint

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21One-day-old broilers were randomly allocated to 5 treatments: basal diet 22 challenged by saline (negative control, n-control); basal diet and challenged by E.coil 23 O 78 (positive control, p-control); supplementation with L. plantarum 15-1 at 1×10 8 24 CFU/kg challenged with E.coil O 78 (LP); supplementation with FOS at 5 g/kg 25 challenged with E.coil O 78 (FOS); supplementation with L. plantarum 15-1 and FOS 26 challenged with E.coil O 78 (LP+FOS). L. plantarum 15-1 or FOS had a lowered effect 27 (P＜0.05) on crypt depth on d 14 compared with two controls, and L. plantarum 15-1, 28 FOS and L. plantarum 15-1+FOS also reduced relative to p-control on d 21. L. 29 plantarum 15-1 reduced the level of diamine oxidase (DAO) at d 14 and 21 compared 30 with p-control (P＜0.05), the broilers with L. plantarum 15-1 and FOS increased the 31 concentration of IgA and IgG relative to two control, and decreased diamine oxidase 32 (DAO) compared with p-control (P＜ 0.05). L. plantarum 15-1 increased the 33 concentration of acetic acid and total short-chain fatty acid (SCFA) in comparison with 34 p-control at d 14 (P＜0.05), FOS improved the level of valeric acid and total SCFA 35 relative to p-control at d 21 (P<0.001), the broilers fed L. plantarum 15-1 and FOS 36 were increased the level of butyric acid at d 14 (P＜0.05). FOS enhanced bursal index 37 of broilers at d 21 (P＜0.05). L. plantarum 15-1 and FOS did no effect on the growth 38 performance. In conclusion, FOS can promote average daily gain, L. plantarum 15-1 39 and FOS can improve intestinal morphology, and increase the concentration of SCFA 40 in cecal contents in broilers challenged with E.coil O 78 . These results suggest that L. 41 plantarum 15-1 and FOS have effective mitigation to E. coil O 78 via lowing reducing 42 the intestinal injury and enhancing the immune responses. 43 Key words: animal models of infection, immunology, animal performance 44 45 Introduction 46 Escherichia coli (E. coli)-induced diarrhoea has become a global public health 47 problem, both in developed and developing countries. At present, the prevention and 48 treatment of the disease is mainly based on drugs and vaccines. In addition, dietary 49intervention has also become an important means [1]. E. coli can produce the 50 enterotoxin that causes colibacillosis, which brings untold damage for the poultry 51 production [2, 3]. E. coli whose serotypes are O1, O2, O78, O15 and O55 had been 52 found to be related to colibacillosis in chickens [4, 5], which may undermine immune 53 function to predispose host animals to the colonization of the pathogens, placing the 54 threat to health and food safety. Although antibiotic therapy is effective for 55 colibacillosis，there are increasing restriction and ban to limit the use of antibiotic to 56 poultry. Therefore, prebiotic and probiotic as candidates to replace antibiotics are 57 available, they are more secure to prevent and control colibacillosis, thus protecting 58 livestock species. Short-chain fatty acids produced by intestinal microbiota are o...

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Advances in vaccination against avian pathogenic Escherichia coli respiratory disease: Potentials and limitations

Cited by 119 publications

References 55 publications

Novel small molecule modulators of quorum sensing in avian pathogenic Escherichia coli (APEC)

Novel small molecule modulators of quorum sensing in avian pathogenic Escherichia coli (APEC)

Exploiting bacterial outer membrane vesicles as a cross-protective vaccine candidate against avian pathogenic Escherichia coli (APEC)

Effects of Lactobacillus plantarum 15-1 and fructooligosaccharide on the response of broilers to pathogenic Escherichia coli O₇₈ challenge

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Advances in vaccination against avian pathogenic Escherichia coli respiratory disease: Potentials and limitations

Cited by 119 publications

References 55 publications

Novel small molecule modulators of quorum sensing in avian pathogenic Escherichia coli (APEC)

Novel small molecule modulators of quorum sensing in avian pathogenic Escherichia coli (APEC)

Exploiting bacterial outer membrane vesicles as a cross-protective vaccine candidate against avian pathogenic Escherichia coli (APEC)

Effects of Lactobacillus plantarum 15-1 and fructooligosaccharide on the response of broilers to pathogenic Escherichia coli O78 challenge

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Resources

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Effects of Lactobacillus plantarum 15-1 and fructooligosaccharide on the response of broilers to pathogenic Escherichia coli O₇₈ challenge