Advances in understanding the fundamental aspects required for successful cryopreservation of Australian flora

Funnekotter, Bryn; Mancera, Ricardo L.; Bunn, Eric

doi:10.1007/s11627-017-9850-5

Cited by 29 publications

(20 citation statements)

References 83 publications

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“…Although a considerable amount of literature has been published on plant genetic stability after cryopreservation [ 2 , 100 , 101 , 102 ], the effect that this process has on epigenetic stability has been scarcely approached. MSAP is one of the techniques most widely used for DNA methylation-related studies of plant material after cryopreservation.…”

Section: Cryopreservationmentioning

confidence: 99%

Application of the MSAP Technique to Evaluate Epigenetic Changes in Plant Conservation

González‐Benito

Ibáñez

Pirredda

et al. 2020

IJMS

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Epigenetic variation, and particularly DNA methylation, is involved in plasticity and responses to changes in the environment. Conservation biology studies have focused on the measurement of this variation to establish demographic parameters, diversity levels and population structure to design the appropriate conservation strategies. However, in ex situ conservation approaches, the main objective is to guarantee the characteristics of the conserved material (phenotype and epi-genetic). We review the use of the Methylation Sensitive Amplified Polymorphism (MSAP) technique to detect changes in the DNA methylation patterns of plant material conserved by the main ex situ plant conservation methods: seed banks, in vitro slow growth and cryopreservation. Comparison of DNA methylation patterns before and after conservation is a useful tool to check the fidelity of the regenerated plants, and, at the same time, may be related with other genetic variations that might appear during the conservation process (i.e., somaclonal variation). Analyses of MSAP profiles can be useful in the management of ex situ plant conservation but differs in the approach used in the in situ conservation. Likewise, an easy-to-use methodology is necessary for a rapid interpretation of data, in order to be readily implemented by conservation managers.

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Section: Cryopreservationmentioning

confidence: 99%

Application of the MSAP Technique to Evaluate Epigenetic Changes in Plant Conservation

González‐Benito

Ibáñez

Pirredda

et al. 2020

IJMS

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“…[9][10][11]. The permeation of small molecules is also critical for assessing the toxicity of exogenous particles such as diesel soot or silica dust [12], the use of nanoparticles for imaging, biosensing and therapeutic applications [13] and the use of organic molecules in the cryopreservation of plant germplasm [14,15]. Consequently, understanding small molecule-membrane interactions (SMMIs) and, in particular, the ability to predict the binding affinity and permeation coefficients of small molecules is an active area of research and aids our understanding of physiological processes and facilitates pharmaceutical development and a diverse range of biotechnological applications.…”

Section: Introductionmentioning

confidence: 99%

Molecular Dynamics Simulation of Small Molecules Interacting with Biological Membranes

et al. 2020

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Cell membranes protect and compartmentalise cells and their organelles. The semi-permeable nature of these membranes controls the exchange of solutes across their structure. Characterising the interaction of small molecules with biological membranes is critical to understanding of physiological processes, drug action and permeation, and many biotechnological applications. This review provides an overview of how molecular simulations are used to study the interaction of small molecules with biological membranes, with a particular focus on the interactions of water, organic compounds, drugs and short peptides with models of plasma cell membrane and stratum corneum lipid bilayers. This review will not delve on other type of membranes which might have different composition and arrangement, such as thylakoid or mitochondrial membranes. The application of unbiased molecular dynamics simulations and enhanced sampling methods such as umbrella sampling, metadynamics and replica exchange are described using key examples. This review demonstrates how state-of-the-art molecular simulations have been used successfully to describe the mechanism of binding and permeation of small molecules with biological membranes, as well as associated changes to the structure and dynamics of these membranes. The review concludes with an outlook on future directions in this field.

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“…Most recalcitrant seeds studied to date are produced by evergreen rainforest tree or shrub species in moist tropical and subtropical zones. These seeds tend to be larger and have a higher water content than orthodox seeds, and are metabolically active and temperature sensitive; these characteristics make them unsuitable for storage by conventional seed banking methods (Tweddle et al 2003;Li and Pritchard 2009;Hamilton et al 2013;Funnekotter et al 2017c). Many threatened species fall under the recalcitrant category and are thus much more suited to other ex situ storage methods such as in vitro (tissue) culture and/or cryopreservation (Ashmore et al 2011).…”

Section: Conservation Options For the Australian Floramentioning

confidence: 99%

“…Tissue culture is a technique used to grow plant cells, tissues and organs on synthetic media in an aseptic environment with controlled light, temperature and humidity conditions (Dagla 2012). Tissue culture allows a large amount of plant material to be produced from a small amount of starting material while maintaining key genotypic features of elite genotypes (or simply a limited number of existing plants in the case of threatened taxa), which may be lost in storage of genetically varying seed accessions (Ashmore et al 2011;Bunn et al 2011;Funnekotter et al 2017c). However, tissue cultures require regular maintenance, may be accidentally contaminated via operator error, may accumulate somaclonal (or epigenetic) variation over many generations of continuous culture cycles, and may decline in viability over time (Larkin and Scowcroft 1981;Miguel and Marum 2011), and the presence of endophytes may hasten the death of the explants by availing more nutrients from either the plant culture medium or damaged tissues (Cassells 1991;Fouda et al 2015).…”

Section: Conservation Options For the Australian Floramentioning

confidence: 99%

“…Oxidative stress constitutes a major component of cryo-injury and is a major obstacle for successful cryopreservation (Benson and Bremner 2004;Chetverikova 2012;Funnekotter et al 2017c). Each of the steps in the vitrification technique of cryopreservation (excision, pre-culture, loading solution, CPA treatment, cooling, rewarming, washing and regrowth) presents the possibility of oxidative stress, due to physical damage and osmotic stress involved in the process, and many studies have shown this (Uchendu et al 2010).…”

Section: Oxidative Stress Damage In Cryopreservationmentioning

confidence: 99%

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Current issues in plant cryopreservation and importance for ex situ conservation of threatened Australian native species

et al. 2019

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An alarming proportion of Australia’s unique plant biodiversity is under siege from a variety of environmental threats. Options for in situ conservation are becoming increasingly compromised as encroaching land use, climate change and introduced diseases are highly likely to erode sanctuaries regardless of best intentions. Ex situ conservation is currently limited to botanic garden living collections and seed banking, with in vitro and cryopreservation technologies still being developed to address ex situ conservation of species not amenable to conventional storage. Cryopreservation (storage in liquid nitrogen) has been used successfully for long-term biosecure storage of shoot tips of several species of threatened Australian plants. We present a case for building on this research and fostering further development and utilisation of cryopreservation as the best means of capturing critical germplasm collections of Australian species with special storage requirements (e.g. recalcitrant-seeded taxa and species with short-lived seeds) that currently cannot be preserved effectively by other means. This review highlights the major issues in cryopreservation that can limit survival including ice crystal damage and desiccation, toxicity of cryoprotective agents, membrane damage, oxidative stress and mitochondrial function. Progress in understanding and mitigating these stresses is vital for advancing cryopreservation for conservation purposes.

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Advances in understanding the fundamental aspects required for successful cryopreservation of Australian flora

Cited by 29 publications

References 83 publications

Application of the MSAP Technique to Evaluate Epigenetic Changes in Plant Conservation

Application of the MSAP Technique to Evaluate Epigenetic Changes in Plant Conservation

Molecular Dynamics Simulation of Small Molecules Interacting with Biological Membranes

Current issues in plant cryopreservation and importance for ex situ conservation of threatened Australian native species

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