Advances in understanding and in multi-disciplinary methodology used to assess lipid regulation of signalling cascades from the cancer cell plasma membrane

Soteriou, Chrysa; Kalli, Antreas C.; Connell, Simon D.; Tyler, Arwen I. I.; Thorne, James L.

doi:10.1016/j.plipres.2020.101080

Cited by 11 publications

(6 citation statements)

References 286 publications

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“… 24 Importantly, these product ions allow unambiguous assignment of db locations to sn -1 acyl chains based on the fragmentation sequence outlined in Figure S12 . For example, in the case of [PC 34:1 + Na] + ( Figure 3 A), the CID/OzID 2 ions observed at m / z 295.12 and 323.15 reveal the presence of lipid isomers carrying both n -7 and n -9 db locations at the sn -1 position, namely, the noncanonical PC 18:1 n -9/16:0 and PC 18:1 n -7/16:0 lipids. Pham et al also identified an alternative MS 4 sequence, which uniquely assigns db isomers of the sn- 2-substituted acyl chains.…”

Section: Resultsmentioning

confidence: 99%

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Mass Spectrometry Imaging of Lipids with Isomer Resolution Using High-Pressure Ozone-Induced Dissociation

et al. 2021

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Mass spectrometry imaging (MSI) of lipids within tissues has significant potential for both biomolecular discovery and histopathological applications. Conventional MSI technologies are, however, challenged by the prevalence of phospholipid regioisomers that differ only in the location(s) of carbon–carbon double bonds and/or the relative position of fatty acyl attachment to the glycerol backbone ( i.e ., sn position). The inability to resolve isomeric lipids within imaging experiments masks underlying complexity, resulting in a critical loss of metabolic information. Herein, ozone-induced dissociation (OzID) is implemented on a mobility-enabled quadrupole time-of-flight (Q-TOF) mass spectrometer capable of matrix-assisted laser desorption/ionization (MALDI). Exploiting the ion mobility region in the Q-TOF, high number densities of ozone were accessed, leading to ∼1000-fold enhancement in the abundance of OzID product ions compared to earlier MALDI-OzID implementations. Translation of this uplift into imaging resulted in a 50-fold improvement in acquisition rate, facilitating large-area mapping with resolution of phospholipid isomers. Mapping isomer distributions across rat brain sections revealed distinct distributions of lipid isomer populations with region-specific associations of isomers differing in double bond and sn positions. Moreover, product ions arising from sequential ozone- and collision-induced dissociation enabled double bond assignments in unsaturated fatty acyl chains esterified at the noncanonical sn -1 position.

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Section: Resultsmentioning

confidence: 99%

“…Lipids, the primary constituents of cell membranes, 1 are key players in many biochemical functions including membrane structure, 2 energy storage, 3 signal transduction, 4 and apoptosis, 5 as well as cancer metabolism. 6 The function and bioactivity of a lipid are dependent on its chemical structure.…”

Section: Introductionmentioning

confidence: 99%

Mass Spectrometry Imaging of Lipids with Isomer Resolution Using High-Pressure Ozone-Induced Dissociation

et al. 2021

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“…In their 2016 review, Yoon and Eom described evidence regarding the use of pharmacological HDACi to reduce risk of several CVD outcomes, such as myocardial infarction and hypertension. However, HDACi is was also linked to worse vascular calcification and indeed, and thrombus was a side effect of significant clinical importance in patients taking vorinostat ( 56 ) . The molecular mechanisms of action of how PSS inhibit HDAC function in the context of CVD therefore require further elucidation.…”

Section: Resultsmentioning

confidence: 99%

Phytosterol and phytostanol-mediated epigenetic changes in cancer and other non-communicable diseases: a systematic review

Jefrei,

Xu,

Moore

et al. 2023

Br J Nutr

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Phytosterols/phytostanols are bioactive compounds found in vegetable oils, nuts, and seeds and added to a range of commercial food products. Consumption of phytosterols/phytostanols reduces levels of circulating low-density lipoprotein cholesterol, a causative biomarker of cardiovascular disease, and is linked to a reduced risk of some cancers. Individuals who consume phytosterols/phytostanols in their diet may do so for many years as part of a non-pharmacological route to lower cholesterol or as part of a healthy diet. However, the impact of long-term or high-intakes of dietary phytosterols/phytostanols has not been on whole-body epigenetic changes before. The aim of this systematic review was to identify all publications that have evaluated changes to epigenetic mechanisms (post-translation modification of histones, DNA methylation, and miRNA expression) in response to phytosterols/phytostanols. A systematic search was performed that returned 226 records, of which eleven were eligible for full text analysis. Multiple phytosterols were found to inhibit expression of histone deacetylase enzymes and were also predicted to directly bind and impair histone deacetylase activity. Phytosterols were found to inhibit the expression and activity of DNA methyl transferase enzyme 1 and reverse cancer associated gene silencing. Finally, phytosterols have been shown to regulate over 200 miRNAs, although only five of these were reported in multiple publications. Five tissue types (breast, prostate, macrophage, aortic epithelia, lung) were represented across the studies, and although phytosterols/phytostanols alter the molecular mechanisms of epigenetic inheritance in these mammalian cells, studies exploring mitotic or transgenerational inheritance were not found.

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“…The PM constantly consists of a phospholipid bilayer structure in which thousands of lipid and protein species are inhomogeneously distributed [225] . Moreover, PM is also an important organelle to sense the extracellular environment as well as participates in signaling pathways [226] . However, the lipid composition of cancer cell PM changes in diverse diseases [227] , so the PM can hopefully become a fresh target of cancer therapy.…”

Section: Organelle-targeted Rtmentioning

confidence: 99%

Targeting the organelle for radiosensitization in cancer radiotherapy

Sun,

Wu,

et al. 2024

Asian Journal of Pharmaceutical Sciences

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Advances in understanding and in multi-disciplinary methodology used to assess lipid regulation of signalling cascades from the cancer cell plasma membrane

Cited by 11 publications

References 286 publications

Mass Spectrometry Imaging of Lipids with Isomer Resolution Using High-Pressure Ozone-Induced Dissociation

Mass Spectrometry Imaging of Lipids with Isomer Resolution Using High-Pressure Ozone-Induced Dissociation

Phytosterol and phytostanol-mediated epigenetic changes in cancer and other non-communicable diseases: a systematic review

Targeting the organelle for radiosensitization in cancer radiotherapy

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