Advances in Ultrahigh-Pressure Liquid Chromatography Technology and System Design

Vos, Jelle De; Broeckhoven, Ken; Eeltink, Sebastiaan

doi:10.1021/acs.analchem.5b04381

Cited by 91 publications

(63 citation statements)

References 144 publications

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“…Furthermore, when raising the pressure limit of the system above 400 bar, radial and axial temperature gradients due to viscous heating will affect the retention properties and/or the efficiency observed. The magnitude strongly depends on the column diameter and also the oven configuration, i.e., a still-air or forced-air oven, utilized [32].…”

Section: Kinetic Performance Limitsmentioning

confidence: 99%

“…Future designs of UHPLC equipment will possibly strive to minimize the number of connections and tubing, i.e., by integrating fluidic pathways, similar to what can be obtained in advanced microfluidic platforms. Major developments include new pump designs that account for solvent compression and heating effects when operating at ultra-high pressure [32]. Applying unique feedback systems, the piston speed can be adjusted as function of gradient composition and pressure to ensure constant flow-rate delivery [41].…”

Section: Advancing Performance Limits and Selected Examplesmentioning

confidence: 99%

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Resolving power in liquid chromatography: A trade‐off between efficiency and analysis time

Dores-Sousa

Vos

Eeltink

2018

J of Separation Science

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This review describes chromatographic dispersion and different plate‐height models frequently used to assess the chromatographic performance of ultra‐high‐pressure liquid chromatography column technology. Furthermore, different performance indices, including the resolution, the separation impedance, and kinetic plots are discussed allowing to quantify and visualize the resolving power in liquid chromatography. The construction of kinetic plots is explained, and different visualization approaches are highlighted. Finally, key instrument and column‐technology developments to advance the kinetic performance limits are discussed and selected state‐of‐the‐art applications are highlighted.

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Section: Kinetic Performance Limitsmentioning

confidence: 99%

Section: Advancing Performance Limits and Selected Examplesmentioning

confidence: 99%

Resolving power in liquid chromatography: A trade‐off between efficiency and analysis time

Dores-Sousa

Vos

Eeltink

2018

J of Separation Science

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“…Over the last years, the influence of the extra-column volume in LC on the obtained chromatographic efficiency has been extensively discussed in the literature when small i.d. columns became commercially available [5,9,[36][37][38][39]. In general, the apparent column efficiency is always negatively affected by the total system variance (² v,ec ) of the LC system including tubing (² v,ec,cap ), injection valve and injection volume (² v,ec,inj ) as well as the detector cell (² v,ec,det ) and becomes more crucial when the peak volumes decrease [5].…”

Section: Extra-column Contribution Of Chromatographic Systemmentioning

confidence: 99%

Characterization of the efficiency of microbore liquid chromatography columns by van Deemter and kinetic plot analysis

et al. 2016

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The efficiency of miniaturized liquid chromatography columns with inner diameters between 200 and 300 μm has been investigated using a dedicated micro-liquid chromatography system. Fully porous, core-shell and monolithic commercially available stationary phases were compared applying van Deemter and kinetic plot analysis. The sub-2 μm fully porous as well as the 2.7 μm core-shell particle packed columns showed superior efficiency and similar values for the minimum reduced plate heights (2.56-2.69) before correction for extra-column contribution compared to normal-bore columns. Moreover, the influence of extra-column contribution was investigated to demonstrate the difference between apparent and intrinsic efficiency by replacing the column by a zero dead volume union to determine the band spreading caused by the system. It was demonstrated that 72% of the intrinsic efficiency could be reached. The results of the kinetic plot analysis indicate the superior performance of the sub-2 μm fully porous particle packed column for ultra-fast liquid chromatography.

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“…This increased capability can intensify temperature effects that occur due to higher flows and pressures. [45,46] To give users flexibility in controlling this heating, column ovens that enable both still-air and forced-air thermal environments are now integrated into some LC instruments. [46,47]…”

Section: Introductionmentioning

confidence: 99%

Repeatability of gradient ultrahigh pressure liquid chromatography–tandem mass spectrometry methods in instrument-controlled thermal environments

Grinias

Wong

Kennedy

2016

Journal of Chromatography A

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The impact of viscous friction on eluent temperature and column efficiency in liquid chromatography is of renewed interest as the need for pressures exceeding 1000 bar to use with columns packed with sub-2 µm particles has grown. One way the development of axial and radial temperature gradients that arise due to viscous friction can be affected is by the thermal environment the column is placed in. In this study, a new column oven integrated into an ultrahigh pressure liquid chromatograph that enables both still-air and forced-air operating modes is investigated to find the magnitude of the effect of the axial thermal gradient that forms in 2.1 × 100 mm columns packed with sub-2 µm particles in these modes. Temperature increases of nearly 30 K were observed when the generated power of the column exceeded 25 W/m. The impact of the heating due to viscous friction on the repeatability of peak capacity, elution time, and peak area ratio to an internal standard for a gradient UHPLC-MS/MS method to analyze neurotransmitters was found to be limited. This result indicates that high speed UHPLC-MS/MS methods under conditions of high viscous friction may be possible without the negative effects typically observed with isocratic separations under similar conditions.

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Advances in Ultrahigh-Pressure Liquid Chromatography Technology and System Design

Cited by 91 publications

References 144 publications

Resolving power in liquid chromatography: A trade‐off between efficiency and analysis time

Resolving power in liquid chromatography: A trade‐off between efficiency and analysis time

Characterization of the efficiency of microbore liquid chromatography columns by van Deemter and kinetic plot analysis

Repeatability of gradient ultrahigh pressure liquid chromatography–tandem mass spectrometry methods in instrument-controlled thermal environments

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