Advances in tooth agenesis and tooth regeneration

Ravi, V.; Murashima-Suginami, Akiko; Kiso, Honoka; Tokita, Yoshihito; Huang, Chenhui; Takagi, Junichi; Sugai, Manabu; Tabata, Yasuhiko; Takahashi, Kazuyuki

doi:10.1016/j.reth.2023.01.004

Cited by 4 publications

(3 citation statements)

References 88 publications

(105 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…via co-culture, co-bioprinting), as well as breakthroughs in identifying and modulating tooth field-forming and -inhibiting signals (e.g. USAG-1, also known as SOSTDC1) will be the necessary next steps forward in the field [ 1 , 110 , 111 ].…”

Section: Discussionmentioning

confidence: 99%

From Pluripotent Stem Cells to Organoids and Bioprinting: Recent Advances in Dental Epithelium and Ameloblast Models to Study Tooth Biology and Regeneration

Hermans,

Hasevoets,

Vankelecom

et al. 2024

Stem Cell Rev and Rep

View full text Add to dashboard Cite

Ameloblasts are the specialized dental epithelial cell type responsible for enamel formation. Following completion of enamel development in humans, ameloblasts are lost and biological repair or regeneration of enamel is not possible. In the past, in vitro models to study dental epithelium and ameloblast biology were limited to freshly isolated primary cells or immortalized cell lines, both with limited translational potential. In recent years, large strides have been made with the development of induced pluripotent stem cell and organoid models of this essential dental lineage – both enabling modeling of human dental epithelium. Upon induction with several different signaling factors (such as transforming growth factor and bone morphogenetic proteins) these models display elevated expression of ameloblast markers and enamel matrix proteins. The advent of 3D bioprinting, and its potential combination with these advanced cellular tools, is poised to revolutionize the field – and its potential for tissue engineering, regenerative and personalized medicine. As the advancements in these technologies are rapidly evolving, we evaluate the current state-of-the-art regarding in vitro cell culture models of dental epithelium and ameloblast lineage with a particular focus toward their applicability for translational tissue engineering and regenerative/personalized medicine. Graphical Abstract Future perspectives for in vitro modeling of dental epithelium and ameloblasts. Development of iPSC and organoid models that can reliably generate dental epithelium and ameloblast-like cells, together with advances in 3D bioprinting, provide promising tools for enamel research. Advanced models will provide new avenues for development of enamel repair/regeneration approaches, for testing of dental materials or drugs, studying host-pathogen and/or cell-cell interactions, in vitro modeling of enamel diseases (e.g. amelogenesis imperfecta) and developing novel insights in fundamental tooth biology (e.g. regulation of amelogenesis, lineage specification). Abbreviations: iPSC: induced pluripotent stem cells; TO: tooth organoids; DE: dental epithelium; AB: ameloblast.

show abstract

Section: Discussionmentioning

confidence: 99%

From Pluripotent Stem Cells to Organoids and Bioprinting: Recent Advances in Dental Epithelium and Ameloblast Models to Study Tooth Biology and Regeneration

Hermans,

Hasevoets,

Vankelecom

et al. 2024

Stem Cell Rev and Rep

View full text Add to dashboard Cite

show abstract

“…As we mentioned above, odontogenic signalling pathways such as WNT [53,73], SHH [126,127], and others have been found in different vertebrates. The polyphyodont potential has been preserved in some mammals, including humans, as a third dentition has been described in some patients [116,128]. Although mice are not the optimal choice for successional tooth studies, they remain one of the most frequently used animal models, and they can be utilized for dental-related research.…”

Section: Discussion and Perspective: Exploring The Dentition Developm...mentioning

confidence: 99%

Count Me in, Count Me out: Regulation of the Tooth Number via Three Directional Developmental Patterns

Fang,

Atukorallaya

2023

IJMS

View full text Add to dashboard Cite

Tooth number anomalies, including hyperdontia and hypodontia, are common congenital dental problems in the dental clinic. The precise number of teeth in a dentition is essential for proper speech, mastication, and aesthetics. Teeth are ectodermal organs that develop from the interaction of a thickened epithelium (dental placode) with the neural-crest-derived ectomesenchyme. There is extensive histological, molecular, and genetic evidence regarding how the tooth number is regulated in this serial process, but there is currently no universal classification for tooth number abnormalities. In this review, we propose a novel regulatory network for the tooth number based on the inherent dentition formation process. This network includes three intuitive directions: the development of a single tooth, the formation of a single dentition with elongation of the continual lamina, and tooth replacement with the development of the successional lamina. This article summarizes recent reports on early tooth development and provides an analytical framework to classify future relevant experiments.

show abstract

“…They can be developed more quickly and cheaply than small molecules and recombinant proteins, and they can be rapidly adapted for personalisation or to target evolving pathogens [ 51 ]. In fact, scientists from Kyoto University are poised to start the first phase 1 human clinical trial assessing antibody and siRNA against USAG-1 to treat anodontia [ 52 , 53 ]. Uterine sensitisation–associated gene-1 ( USAG-1 ) deficiency is associated with supernumery tooth formation via enhanced bone morphogenetic protein (BMP) signalling.…”

Section: Scientific Progress In Dental Omicsmentioning

confidence: 99%

Bridging the gap between omics research and dental practice

Kabbashi,

Roomaney,

Chetty

2024

BDJ Open

View full text Add to dashboard Cite

Aim The burgeoning field of omics research has witnessed exponential growth in both medicine and dentistry. However, despite more than a decade of advancements, clinical dentistry, particularly in Low- and Middle-Income Countries (LMICs), has seen limited progress in integrating omics-based approaches into routine practice. This review aims to provide a comprehensive overview of the integration of omics approaches in dentistry, focusing on the challenges and opportunities for translating research findings into clinical practice. Methods we conducted a literature review using key databases to provide a brief overview of the history of genomics in dentistry. Additionally, we summarised recent breakthroughs in omics relevant to oral health practitioners, emphasising the inadequate translation of omics research into clinical practice. Results Despite significant growth in omics research in both medicine and dentistry, its translation into routine clinical practice in dentistry remains limited. We summarise recent breakthroughs in omics and highlight the gap between research advancements and clinical implementation. Discussion and conclusion The integration of omics approaches holds promise for enhancing diagnostics, personalised treatment strategies, and preventive measures in dental practice, ushering in a new era of precision oral healthcare. However, several challenges, including infrastructure limitations, cost-effectiveness, and education gaps, hinder the widespread adoption of omics-based approaches in clinical dentistry. A strong commitment to transforming dentistry is required to embrace this transition. This shift has the potential to revolutionise oral healthcare by advancing precision diagnostics and treatment strategies tailored to individual patient needs.

show abstract

Advances in tooth agenesis and tooth regeneration

Cited by 4 publications

References 88 publications

From Pluripotent Stem Cells to Organoids and Bioprinting: Recent Advances in Dental Epithelium and Ameloblast Models to Study Tooth Biology and Regeneration

From Pluripotent Stem Cells to Organoids and Bioprinting: Recent Advances in Dental Epithelium and Ameloblast Models to Study Tooth Biology and Regeneration

Count Me in, Count Me out: Regulation of the Tooth Number via Three Directional Developmental Patterns

Bridging the gap between omics research and dental practice

Contact Info

Product

Resources

About