Advances in Tissue Culture of Cucurbits: A Review

Dhumal, Sangram; Naik, Bitan; Nimbalkar, Mansinghraj S.

doi:10.20546/ijcmas.2020.908.324

Cited by 4 publications

(4 citation statements)

References 30 publications

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“…propagation with shoot tips and cotyledonal nodes obtained from seedlings and (b) other morphogenic processes such as organogenesis. However, there are few studies carried out on Cucurbita species, as evidenced by a recent review on tissue culture of cucurbits (Dhumal et al, 2020) where no more than five studies were carried out on C. maxima, C. pepo and C. ficifolia Bouché, 1837.…”

Section: Disinfestation Of 'Loche' Explants For In Vitro Culturesmentioning

confidence: 99%

“…Also, the efficiency of haploidization in C. maxima and C. moschata through another culture was evaluated, reaching up to 47.3% of haploid plants (n=20) (Kurtar et al, 2016). An extensive review of advances in tissue culture of cucurbits has been recently published (Dhumal et al, 2020). In recent years, the literature does not report studies on micropropagation of C. moschata and C. ecuadorensis.…”

Section: Introductionmentioning

confidence: 99%

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Protocol for ex vitro and in vitro micropropagation of Cucurbita moschata and C. ecuadorensis, native to Peru and Ecuador, of nutritional and medicinal importance

2023

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Cucurbitaceae species constitute a family with a wide worldwide distribution and great nutritional and medicinal importance. The Cucurbita genus is the most important of the family with valuable species such as C. maxima and C. moschata. However, the large-scale production of sex specific plants using the conventional propagation methods has various limitations. The tissue culture such as micropropagation can help overcome these limitations. The aim of this study was to establish a protocol for ex vitro and in vitro clonal propagation of two varieties of Cucurbita moschata: ‘loche’, a variety of commercial vegetative propagation and endemic in Lambayeque, and ‘chuyán’ another variety of very restricted cultivation in some Andean regions of the north of the Peru. Likewise, the in vitro propagation of ‘chisguín’ (C. ecuadorensis), a threatened endemic wild species from the coast of Ecuador. ‘Loche’ was propagated ex vitro by microcutting with a node and leaf, and micropropagated in MS culture medium supplemented with 0.02 mg L-1 IAA and 0.02 mg L-1 GA3. Seedlings of ‘chuyán’ and ‘chisguin’ were micropropagated in MS with with 0.02 mg L-1 IAA, 0.5 mg L-1 BAP and 0.02 mg L-1 GA3. This study constitutes the first report on micropropagation of Peruvian and Ecuadorian varieties and species of the Cucurbita genus.

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Section: Disinfestation Of 'Loche' Explants For In Vitro Culturesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Protocol for ex vitro and in vitro micropropagation of Cucurbita moschata and C. ecuadorensis, native to Peru and Ecuador, of nutritional and medicinal importance

2023

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“…Due to the limitations of the aforementioned methods, researchers need to find a more efficient and stable propagation method for T. cucumerina, such as plant tissue culture. Plant tissue culture technology plays a crucial role in germplasm innovation, breeding, and germplasm resource preservation, serving as the primary and most effective method for industrial seedling production of T. cucumerina (Kyte et al 2013;Bridgen et al 2018;Dhumal et al 2020). Commonly used explants for in vitro culture of Trichosanthes include seeds (Hwang 2007), shoot tips (Devendra et al 2008), stem segments (Joo et al 2019;Cai et al 2019), and leaves (Zhao et al 2018); Xu et al (2015) even successfully achieved in vitro regeneration of T. cucumerina by inducing somatic embryos.…”

Section: Introductionmentioning

confidence: 99%

In vitro cluster buds regeneration and control of Shoot Tip Necrosis in tissue cultures of Trichosanthes cucumerina Linn.

LIU,

DENG,

YAN

et al. 2024

Preprint

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Trichosanthes cucumerina Linn. is a medicinal vine with great potential that can be used as a vegetable. In this study, an efficient in vitro regeneration system of T. cucumerina was established by inducing adventitious buds and controlling Shoot Tip Necrosis (STN) in vitro culture. The results of hormone single factor and orthogonal experiments showed that 6-BA had a significant effect on inducing adventitious buds. The best medium for bud proliferation was MS + 6-BA 3.0 mg·L-1 + KT 2.0 mg·L-1 + NAA 0.5 mg·L-1, and the proliferation coefficient was 14.68. However, after three consecutive subcultures, a higher incidence of STN (78.86%) was observed. The results of the STN control experiment showed that reducing the concentration of inorganic salt, shortening the subculture interval, and increasing the Ca2+ in the medium could effectively control the occurrence of STN. When the subculture interval was 40 d, the stem segments obtained the lowest STN incidence (8.3%) and higher shoot proliferation coefficient (10.02) on the medium of MS + 3.0 mg·L-1 6-BA + 2.0 mg·L-1 KT + 0.5 mg·L-1 NAA + 360.36 mg·L-1 Ca2+. The best rooting medium for stem segments was MS + 0.50 mg·L-1 NAA; the rooting rate was 100%, and no STN occurred. After acclimation, the survival rate of the rooted plantlet reached 95% after 40 days in the soil matrix of coconut husk: humus soil: perlite = 2: 2: 1 (v/v). The results of this study not only reveal the mechanism of STN in T. cucumerina, but also provide technical support and a theoretical basis for its further artificial breeding and cultivation.

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“…Despite its potential, the success of embryo rescue in cucurbits depends on various factors, including the genotype of the parental plants, the developmental stage of the embryos, and the optimization of culture conditions [23,28,29]. Furthermore, advancements in tissue culture techniques, nutrient formulations, and growth regulator applications continue to enhance embryo rescue efficiency and success rate in cucurbits [30,31].…”

Section: Introductionmentioning

confidence: 99%

Widening Genetic Diversity Using Embryo Rescue in Cucurbit Crops: A Review

Reddy,

Ramireddy,

Reddy

2024

Plants

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Embryo rescue is a vital technique in cucurbit breeding and propagation, addressing challenges such as embryo abortion, poor seed viability, and incompatibility barriers. This method involves the excision of immature embryos from seeds followed by their in vitro culture on a nutrient medium, providing an environment conducive to their growth and development. In cucurbits, embryo rescue has been extensively utilized to overcome barriers to hybridization, enabling the production of interspecific and intergeneric hybrids with desired traits. Various factors, including genotype, developmental stage of embryos, and culture conditions, influence the success of embryo rescue in cucurbits. Optimal nutrient formulations, growth regulators, and culture techniques are critical for promoting embryo germination, shoot elongation, and subsequent plantlet establishment. Additionally, embryo rescue facilitates the recovery of valuable genetic material from wild and exotic cucurbit species, expanding genetic diversity and developing novel cultivars with improved traits such as disease resistance, yield, and quality. This review highlights the principles, applications, and advancements in embryo rescue technology in cucurbits, emphasizing its significance in cucurbit breeding programs and crop improvement efforts.

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Advances in Tissue Culture of Cucurbits: A Review

Cited by 4 publications

References 30 publications

Protocol for ex vitro and in vitro micropropagation of Cucurbita moschata and C. ecuadorensis, native to Peru and Ecuador, of nutritional and medicinal importance

Protocol for ex vitro and in vitro micropropagation of Cucurbita moschata and C. ecuadorensis, native to Peru and Ecuador, of nutritional and medicinal importance

In vitro cluster buds regeneration and control of Shoot Tip Necrosis in tissue cultures of Trichosanthes cucumerina Linn.

Widening Genetic Diversity Using Embryo Rescue in Cucurbit Crops: A Review

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