Advances in thin-layer chromatography

Kirchner, J. G.

doi:10.1016/s0021-9673(01)85609-0

Cited by 26 publications

(29 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The lipids were found in the form of brown-yellow spots or stripes. Spots were carefully outlined with a soft pencil and it was waited for the iodine to disappear [16].…”

Section: Detection Of Lipidsmentioning

confidence: 99%

See 2 more Smart Citations

Effect of hypoxia on the composition and state of lipids and oxygen-transport properties of erythrocyte haemoglobin

Ревин

Grunyushkin

Gromova

et al. 2016

Biotechnology & Biotechnological Equipment

View full text Add to dashboard Cite

The aim of this study was to investigate the relationship between the composition and state of erythrocytic lipids and oxygen-binding properties of erythrocyte haemoglobin-derived haematoporphyrin under hypoxia. The results from this study showed that hypoxia causes a profound change in the composition of the lipids that form the bilayer of erythrocyte membranes, as well as in the functional characteristics of erythrocytes. The detected changes correlate with morphological reconstruction of erythrocytes and, consequently, affect the oxygen-transport properties of erythrocytes. The obtained results showed that the lipid phase plays an important role in the functioning of erythrocytes in health as well as in event of development of ischemic heart disease and other pathological processes that accompany hypoxia.

show abstract

“…The lipids were found in the form of brown-yellow spots or stripes. Spots were carefully outlined with a soft pencil and it was waited for the iodine to disappear [16].…”

Section: Detection Of Lipidsmentioning

confidence: 99%

“…This mixture was used to sprinkle the plates. Choline-containing lipids appeared as orange spots [16].…”

Section: Detection Of Lipidsmentioning

confidence: 99%

See 1 more Smart Citation

Effect of hypoxia on the composition and state of lipids and oxygen-transport properties of erythrocyte haemoglobin

Ревин

Grunyushkin

Gromova

et al. 2016

Biotechnology & Biotechnological Equipment

View full text Add to dashboard Cite

show abstract

“…When necessary, they were developed with diphenylamine reagent. 3 Anhydrous magnesium sulfate was used as a drying agent for the organic phases. Organic solvents were removed under vacuum at room temperature.…”

Section: Materials and Methods Generalmentioning

confidence: 99%

Synthesis, chiral HPLC resolution and configuration assignment of 1‐phenylglyceryl trinitrate stereomers

et al. 2006

View full text Add to dashboard Cite

As an introductory study of in vitro vasodilating activity, the access to the four stereomers of 1-phenylglyceryl trinitrate is described using achiral and chiral chromatography. For semi-preparative separation of the enantiomers, a Chiralcel OD (250 3 10 mm, 10 lm) was used. Catalytic reduction leading to the corresponding stereomers of 1-phenylglycerol allowed absolute configuration assignments. The same methods were used for the separation and configuration assignment of the enantiomers of 3-phenylpropane-1,2-diyl dinitrate. The organic esters of nitric acid, the nitrates, are a heterogeneous class of products widely used for the treatment of a number of cardiovascular diseases, including congestive heart failure and coronary heart disease. 1These products act through a common mechanism which implies the activation of the soluble guanylate cyclase (sGC), with consequent increase of intracellular cGMP. This increase in the vascular smooth muscle results in vasodilation. The prototype of nitrates is glyceryl trinitrate (GTN, nitroglycerin). It was introduced in therapy for relief of angina pectoris pain in 1876.2 The major limit in the practical application of this drug is an early development of the tolerance. In this article, we describe the synthesis, isolation, and configuration assignment of the four stereomers of the new nitrate 3 (Ph-GTN) obtained by introducing a phenyl ring on the terminal methylene group of achiral GTN. The related nitrate 5 formally obtained from 3 by elimination of one nitrooxy groups is also described (Scheme 1).The overall Energy Release Potential (ERP), a parameter that evaluates the explosive hazard of a compound, calculated using the ASTM Program CHEAT AH 7.2, was found to be high for compound 3, as well as for the other nitric esters studied in the present work. Consequently, we always treated only small amounts of these substances with a great caution, although we never observed violent decomposition induced by thermal and mechanical shock. MATERIAL AND METHODS GeneralAll compounds were routinely checked by IR (Shimadzu FT-IR 8101 M). 1 H and 13 C-NMR spectra were recorded on a Bruker Avance 300 at 300 and 75 MHz, respectively, using Si(CH 3 ) 4 as an internal standard. The following abbreviations were used to indicate the peak multiplicity: s ¼ singlet, d ¼ doublet, and m ¼ multiplet. Low-resolution mass spectra were recorded with a Finnigan-Mat TSQ-700. Mass spectra in Desorption Chemical Ionization (DCI) of the derivatives 3a, 3b were obtained with a Finnigan-Mat 8400 spectrometer using iso-butane as reacting gas. Accurate molecular mass of 5 was determined by peak matching using perfluorokerosene (PFK) as an internal standard on a Finnigan-Mat 8400 spectrometer. Melting points were determined with a capillary apparatus (Büchi 540) and are uncorrected. Flash column chromatography was performed on silica gel (Merck Kieselgel 60, 230-400 mesh ASTM) using the indicated eluents. Petroleum ether 40-608C (PE) was used as coeluent. The progress of the reactions was follow...

show abstract

“…The procedure of King [15] for quantitation of areas of radioactivity was followed. Plates spotted with a standard solution containing glycolate, glyoxylate, glycine, and serine were chromatographed simultaneously and were stained by spraying with 0.1 %, dichloro-indophenol in ethanol [16].…”

Section: Analysis Of the Products Of Photosynthesismentioning

confidence: 99%

Characterization of the Oxygenase Activity in a Mutant of Chlamydomonas reinhardi Exhibiting Altered Ribulosebisphosphate Carboxylase

Nelson

Surzycki

1976

European Journal of Biochemistry

View full text Add to dashboard Cite

A previously described Mendelian mutant of Chlamydomonas reinhardi, ac i72, exhibiting altered ribulosebisphosphate carboxylase activity and unable to grow on minimal medium is examined for changes in ribulosebisphosphate oxygenase activity. The ribulosebisphosphate oxygenase activity of the enzyme purified from both wild type and ac i72 is compared over a pH range from 7.0 to 9.5. Both enzymes exhibit maximum activity at pH 9.0. However, the ac i72 enzyme is twice as active as the wild type enzyme at a physiological pH of 7.0.The studies in vivo of the products of CO, fixation of ac i72 and wild type cells in the presence of high and low 0, concentration shows that due to a lower level of carboxylation, the ac i72 cells fix CO, at half the rate of wild type cells. In ac i72,24 % of the photosynthetically fixed I4C is channelled into the water-soluble fraction as opposed to 6 %in wild type. Thin-layer chromatography of the watersoluble fraction showed extensive accumulation of components of the glycolate pathway in ac i72 as compared to wild type. This indicates that the oxygenase activity of the enzyme prevails in ac i72 in vivo. Since a high concentration of glycolate is toxic to cells of C . reinhardi, the high oxygenase activity of ac i72 provides an explanation for the inability of ac i72 to grow phototrophically even though its rate of CO, fixation is half that of wild type. This toxicity to glycolate is overcome by growth under amber illumination or low 0, concentration.Glycolate was noted among the primary products of photosynthesis by Benson and Calvin [l] and has now been established as one of the main photorespiratory intermediates. However, its mechanism of synthesis has not yet been fully resolved. Several hypotheses have been proposed, one of which suggests that Rbu-l,5-P2 carboxylase acts not only as the primary enzyme in photosynthesis but also as the primary enzyme in photorespiration, and as such functions as Rbu-1,5-P2 oxygenase catalyzing the condensation of Rbu-1,5-P2 and 0, to form phosphoglycerate and phosphoglycolate [2 -51. A specific phosphoglycolate phosphatase, known to be present in the chloroplast, then catalyzes the production of glycolate from phosphoglycolate [6,7]. This scheme, which involves a competitive reaction between C 0 2 and 0, for the same enzyme [3,8], provides an explanation for the Warburg effect of 0, inhibition of photosynthesis and stimulation of photorespiration, the direct relationship between rates of photosynthesis and photorespiration, the light dependence of photo-' respiration and a site of O2 uptake in photorespiration.The oxygenase function of Rbu-1 ,5-P, carboxylase has been demonstrated in the following ways: (a) the two activities copurify to electrophoretic homogeneity [4], (b) the oxygenase activity, as measured by O2 uptake, is dependent upon Rbu-1,5-P2, 02, and enzyme [4], (c) kinetic studies show the competitive nature of O2 and C 0 2 in both carboxylase and oxygenase reactions [8], (d) the catalytic activity of both reactions resides in the larg...

show abstract

Advances in thin-layer chromatography

Cited by 26 publications

References 0 publications

Effect of hypoxia on the composition and state of lipids and oxygen-transport properties of erythrocyte haemoglobin

Effect of hypoxia on the composition and state of lipids and oxygen-transport properties of erythrocyte haemoglobin

Synthesis, chiral HPLC resolution and configuration assignment of 1‐phenylglyceryl trinitrate stereomers

Characterization of the Oxygenase Activity in a Mutant of Chlamydomonas reinhardi Exhibiting Altered Ribulosebisphosphate Carboxylase

Contact Info

Product

Resources

About