Advances in the Study of Respiration-Deficient (Rd) Mutation in Yeast and Other Microorganisms1

Nagai, S.; Yanagishima, N.; Nagai, H.

doi:10.1128/mmbr.25.4.404-426.1961

Cited by 80 publications

(19 citation statements)

References 77 publications

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“…RD mutants may demonstrate normal, reduced, or increased rates of glycolysis. [7][8][9][10][11][12][13][14][15][16][17][18] This agrees well with the activity of the dehydrogenases involved in glycolysis which may also be normal" or increased. 25 Because of the variations of spontaneous mutation frequency to respiratory deficiency of different strains, four strains of bottom brewer's yeast used in Czech breweries were tested for the presence of RD mutants after fermentations at 6°and 28°C.…”

Section: Introduction Strain Of Saccharomyces Cerevisiaem And-50%supporting

confidence: 65%

Respiratory Deficient Mutants of Bottom Brewer's Yeast. I. Frequencies and Types of Mutant in Various Strains

Šilhánková

Šavel

̆stek

1970

Journal of the Institute of Brewing

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Four strains of bottom brewer's yeast used in Czech breweries did not differ substantially from one another in their frequencies of spontaneous respiratorydeficient (RD) mutants as tested by the triphenyl tetrazolium chloride (TTC) overlay technique. These frequencies amounted to 1.1-1.4% of the total viable cell number after cultivation in bottom layers of wort. The strain which had the highest rate of aerobic growth gave a spontaneous mutation frequency of 0.5% in shaken cultures.In comparison with the TTC overlay technique, YVL-Difco-agar with brom cresol green gave lower estimations of the frequency of production of spontaneous RD mutants.The RD mutants isolated can be divided into 11 groups on the basis of their cytochrome content (particularly cytochromes 6 and c) after aerobic growth.Of the 34 RD isolates tested, only 3 produced considerable numbers of revertants to respiratory sufficiency. These 3 mutants were derived from the same original strain, had the same cytochrome type, and had very slow growth rates.All RD mutants isolated had slower growth rates under aerobic conditions and reduced fermentation rates in static cultures in comparison with the parent types.

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Section: Introduction Strain Of Saccharomyces Cerevisiaem And-50%supporting

confidence: 65%

Respiratory Deficient Mutants of Bottom Brewer's Yeast. I. Frequencies and Types of Mutant in Various Strains

Šilhánková

Šavel

̆stek

1970

Journal of the Institute of Brewing

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“…Acriflavin is one of the most effective agents for production of the "petit" or respiratory-deficient ( R D ) strains of yeast (3,12). Its effective concentration is here again in the same range (around 0.1 mg per 1) as for Bodo(l7) and for L. tarentolae.…”

Section: Figmentioning

confidence: 89%

“…Its effective concentration is here again in the same range (around 0.1 mg per 1) as for Bodo(l7) and for L. tarentolae. The R D mutants of yeast, which lack cytochromes a and b and cannot respire, have abnormal mitochondria (12). The effect of acriflavin on the fine structure of mitochondria of L. tarentolae suggests the possibility of analogous metabolic lesions to those in yeast.…”

Section: Figmentioning

confidence: 99%

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The Riboflavin Requirement and the Effects of Acriflavin on the Fine Structure of the Kinetoplast of Leishmania tarentolae*

Trager¹,

Rudzinska²

1964

The Journal of Protozoology

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SYNOPSIS. Leishmania tarentolae requires at least 10 mμg riboflavin per ml for optimal growth on successive subculture in a denned medium. At this level many organisms are aflagellate or with a short flagellum; higher concentrations of riboflavin did not increase the yield of leptomonads but did increase the proportion with a flagellum at least as long as the body. At limiting levels of riboflavin, acriflavin markedly inhibited growth without producing akinetoplastic forms. With riboflavin at 20 mμ/ml or higher, acriflavin at around 500 mμg/ml permitted an approximately 10‐fold multiplication. Seventy % of the leptomonads formed were akinetoplastic and they could not give rise to subcultures. In electron micrographs of acriflavin‐treated organisms the nucleus was unaffected but the cytoplasmic organelles, notably the kinetoplast and mitochondria, were altered. The mitochondria appeared swollen with their cristae replaced by circular profiles. In the kinetoplast the dense fibrillar material (probably DNA) lost its structure, became diffuse, and eventually disappeared. The observed changes again indicate close relationship between the kinetoplast and the mitochondria of hemoflagellates.

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“…It was found that carcinogenic agent, 4-NQO, could induce the appearance of respiration-deficient (RD) mutant of S. cerevisiae. RD mutants of yeast were induced by acriflavine according to Ephrussi et al(18,19), Nagai and Yanagishima (20) studied also the induction of RD mutant of yeast by many basic dyestuffs. Similar mutants of yeast with deficient respiration have also been obtained by many methods; UV irradiation by Raut(21), camphor treatment by Gause(22), and heat shock by Yeas (23) and Gutz(24).…”

mentioning

confidence: 99%

INDUCTION OF RESPIRATION‐DEFICIENT MUTANT OF SACCHAROMYCES CEREVISIAE BY CARCINOGENIC AGENT, 4‐NITROQUINOLINE N‐OXIDE AND THE EXISTENCE OF TOXOHORMONE‐LIKE FACTOR IN THE MUTANT

Mifuchi

Morita

Yanagihara

et al. 1963

Japanese Journal of Microbiology

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Advances in the Study of Respiration-Deficient (Rd) Mutation in Yeast and Other Microorganisms1

Cited by 80 publications

References 77 publications

Respiratory Deficient Mutants of Bottom Brewer's Yeast. I. Frequencies and Types of Mutant in Various Strains

Respiratory Deficient Mutants of Bottom Brewer's Yeast. I. Frequencies and Types of Mutant in Various Strains

The Riboflavin Requirement and the Effects of Acriflavin on the Fine Structure of the Kinetoplast of Leishmania tarentolae*

INDUCTION OF RESPIRATION‐DEFICIENT MUTANT OF SACCHAROMYCES CEREVISIAE BY CARCINOGENIC AGENT, 4‐NITROQUINOLINE N‐OXIDE AND THE EXISTENCE OF TOXOHORMONE‐LIKE FACTOR IN THE MUTANT

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