Advances in the Production and Batch Reformatting of Phage Antibody Libraries

Abstract

Phage display antibody libraries have proven an invaluable resource for the isolation of diagnostic and potentially therapeutic antibodies, the latter usually being antibody fragments converted into IgG formats. Recent advances in the production of highly diverse and functional antibody libraries are considered here, including for Fabs, scFvs and nanobodies. These advances include codon optimisation during generation of CDR diversity, improved display levels using novel signal sequences, molecular chaperones and isomerases and the use of highly stable scaffolds with relatively high expression levels. In addition, novel strategies for the batch reformatting of scFv and Fab phagemid libraries, derived from phage panning, into IgG formats are described. These strategies allow the screening of antibodies in the end-use format, facilitating more efficient selection of potential therapeutics.

Shark New Antigen Receptor (IgNAR): Structure, Characteristics and Potential Biomedical Applications

“…Fab portion contains one constant domain of the heavy chain (C1) and one constant domain of the light chain (CL), as well as one variable domain of heavy chain (VH) and one variable domain of light chain (VL). The variable domain of each chain is responsible for antigen interactions due to the existence of paratope (the antigen-binding site) [ 1 , 2 ]. The variable domains of IgG are linked by a flexible peptide into an antiparallel sheet ( Figure 1 A).…”

Recombinant Antibodies in Basic Neuroscience Research

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