Advances in the Fabrication of Biomaterials for Gradient Tissue Engineering

Li, Chunching; Ouyang, Liliang; Armstrong, James R.; Stevens, Molly M.

doi:10.1016/j.tibtech.2020.06.005

Cited by 120 publications

(98 citation statements)

References 91 publications

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“…Recent technological advances in biomanufacturing have enabled the biofabrication of biomaterials with differentially arranged growth factor gradients. These advanced techniques include 3D bioprinting, microfluidics, layer-by-layer scaffolding, and techniques that utilize magnetic or electrical fields to distribute biomolecules within scaffolds ( Figure 9 C) [ 166 , 167 ]. Layer-by-layer (LbL) scaffolding has been utilized to create multilayered scaffolds embedded with several growth factors.…”

Section: Encapsulation Incorporation and Related Delivery Stratementioning

confidence: 99%

Advances in Growth Factor Delivery for Bone Tissue Engineering

Oliveira

Nie

Podstawczyk

et al. 2021

IJMS

118

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Shortcomings related to the treatment of bone diseases and consequent tissue regeneration such as transplants have been addressed to some extent by tissue engineering and regenerative medicine. Tissue engineering has promoted structures that can simulate the extracellular matrix and are capable of guiding natural bone repair using signaling molecules to promote osteoinduction and angiogenesis essential in the formation of new bone tissues. Although recent studies on developing novel growth factor delivery systems for bone repair have attracted great attention, taking into account the complexity of the extracellular matrix, scaffolding and growth factors should not be explored independently. Consequently, systems that combine both concepts have great potential to promote the effectiveness of bone regeneration methods. In this review, recent developments in bone regeneration that simultaneously consider scaffolding and growth factors are covered in detail. The main emphasis in this overview is on delivery strategies that employ polymer-based scaffolds for spatiotemporal-controlled delivery of both single and multiple growth factors in bone-regeneration approaches. From clinical applications to creating alternative structural materials, bone tissue engineering has been advancing constantly, and it is relevant to regularly update related topics.

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Section: Encapsulation Incorporation and Related Delivery Stratementioning

confidence: 99%

Advances in Growth Factor Delivery for Bone Tissue Engineering

Oliveira

Nie

Podstawczyk

et al. 2021

IJMS

118

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“…[6,7] More recently, researchers have developed other precise patterning techniques-buoyancy, acoustic, and magnetic patterning-that work across a wide range of materials (e.g., hydrogels) and objects (e.g., cells, growth factors, microspheres, etc.). [8][9][10][11][12] These methods rely on a differential between the object and material property of interest, x, where x refers to the density (buoyancy patterning), density and compressibility (acoustic patterning), or magnetic susceptibility (magnetic patterning). Unlike unidirectional buoyancy-driven gradients and geometric acoustic patterns, magnetic fields have the potential to create multidirectional 3D patterns.…”

Section: Doi: 101002/adma202005030mentioning

confidence: 99%

“…objects are receptive to magnetic tags; for example, intracellular iron oxide particles can compromise cell differentiation. [10,12,13] Ideally, an object could be magnetically manipulated without altering its intrinsic magnetic character.…”

Section: Doi: 101002/adma202005030mentioning

confidence: 99%

Magneto‐Driven Gradients of Diamagnetic Objects for Engineering Complex Tissues

et al. 2020

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Engineering complex tissues represents an extraordinary challenge and, to date, there have been few strategies developed that can easily recapitulate native‐like cell and biofactor gradients in 3D materials. This is true despite the fact that mimicry of these gradients may be essential for the functionality of engineered graft tissues. Here, a non‐traditional magnetics‐based approach is developed to predictably position naturally diamagnetic objects in 3D hydrogels. Rather than magnetizing the objects within the hydrogel, the magnetic susceptibility of the surrounding hydrogel precursor solution is enhanced. In this way, a range of diamagnetic objects (e.g., polystyrene beads, drug delivery microcapsules, and living cells) are patterned in response to a brief exposure to a magnetic field. Upon photo‐crosslinking the hydrogel precursor, object positioning is maintained, and the magnetic contrast agent diffuses out of the hydrogel, supporting long‐term construct viability. This approach is applied to engineer cartilage constructs with a depth‐dependent cellularity mirroring that of native tissue. These are thought to be the first results showing that magnetically unaltered cells can be magneto‐patterned in hydrogels and cultured to generate heterogeneous tissues. This work provides a foundation for the formation of opposing magnetic‐susceptibility‐based gradients within a single continuous material.

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“…Developing fabrication methods to create materials with biologically-relevant, complex, and dynamic gradients has become increasingly important for the tissue engineering field [11]. State-of-the-art strategies include electrospinning, microfluidics, 3D printing, component redistribution, and controlled phase changes [11][12][13]. Our lab has developed a novel method to spatially present different functional groups in a single, continuous scaffold by solvent-cast 3D printing with peptide-polymer How to cite this article: Camacho P, Fainor M, Seims KB, Tolbert JW, Chow LW.…”

Section: Introductionmentioning

confidence: 99%

Fabricating spatially functionalized 3D-printed scaffolds for osteochondral tissue engineering

Camacho¹,

Fainor²,

Seims³

et al. 2021

J Biol Methods

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Three-dimensional (3D) printing of biodegradable polymers has rapidly become a popular approach to create scaffolds for tissue engineering. This technique enables fabrication of complex architectures and layer-by-layer spatial control of multiple components with high resolution. The resulting scaffolds can also present distinct chemical groups or bioactive cues on the surface to guide cell behavior. However, surface functionalization often includes one or more post-fabrication processing steps, which typically produce biomaterials with homogeneously distributed chemistries that fail to mimic the biochemical organization found in native tissues. As an alternative, our laboratory developed a novel method that combines solvent-cast 3D printing with peptide-polymer conjugates to spatially present multiple biochemical cues in a single scaffold without requiring post-fabrication modification. Here, we describe a detailed, stepwise protocol to fabricate peptide-functionalized scaffolds and characterize their physical architecture and biochemical spatial organization. We used these 3D-printed scaffolds to direct human mesenchymal stem cell differentiation and osteochondral tissue formation by controlling the spatial presentation of cartilage-promoting and bone-promoting peptides. This protocol also describes how to seed scaffolds and evaluate matrix deposition driven by peptide organization.

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Advances in the Fabrication of Biomaterials for Gradient Tissue Engineering

Cited by 120 publications

References 91 publications

Advances in Growth Factor Delivery for Bone Tissue Engineering

Advances in Growth Factor Delivery for Bone Tissue Engineering

Magneto‐Driven Gradients of Diamagnetic Objects for Engineering Complex Tissues

Fabricating spatially functionalized 3D-printed scaffolds for osteochondral tissue engineering

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