Advances in synthetic peptides reagent discovery

Adams, Bryn L.; Sarkes, Deborah A.; Finch, Amethist S.; Stratis‐Cullum, Dimitra N.

doi:10.1117/12.2017089

Cited by 1 publication

(1 citation statement)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Following their selection, further characterization experiments have been applied to determine their binding kinetics, thermodynamics and surface stability [24]. There have been several studies selecting new peptides as well as demonstrating their use, including assembly of nanoparticles (NPs) and biomolecules, designing bioactive surfaces or antimicrobial coatings, synthesis of inorganic materials, and developing nanostructured platforms [27][28][29][30][31][32][33][34][35][36][37]. Entrapment of the assembly of engineered proteins bound to the metal nanoparticles as well as minerals in the nanofibers was explored by integrating the fluorescent activity of the coupled proteins in the nanoassembly [38,39].…”

Section: Introductionmentioning

confidence: 99%

Self-Assembled Recombinant Proteins on Metallic Nanoparticles as Bimodal Imaging Probes

Yuca

Tamerler

2019

JOM

View full text Add to dashboard Cite

Combining multiple modalities is at the center of developing new methods for sensing and imaging that are required for comprehensive understanding of events at the molecular level. Various imaging modalities have been developed using metallic nanoparticles owning to their exceptional physical and chemical properties. Due to their localized surface plasmon resonance characteristics, gold and silver nanoparticles exhibit unique optoelectronic properties commonly used in biomedical sciences and engineering. Self assembled monolayers or physical adsorption have previously been adapted to functionalize the surfaces of nanoparticles with biomolecules for targeted imaging. However, depending on differences among the functional groups used on the nanoparticle surface, wide variation in the displayed biomolecular property to recognize its target may result. In the last decade, the properties of inorganic binding peptides have been proven advantageous to assemble selective functional nano-entities or proteins onto nanoparticles surfaces. Herein we explored formation of self-assembled hybrid metallic nano-architectures that are composed of gold and silver nanoparticles with fluorescent proteins, for use as bimodal imaging probes. We employed metal binding peptide-based assembly to self assemble green fluorescence protein onto metallic substrates of various geometries. Assembly of the green fluorescent proteins, genetically engineered to incorporate gold-or silver-binding peptides onto metallic nanoparticles, resulted in the generation of hybrid-, biomodal-imaging probes in a single step. Green fluorescent activity on gold and silver surfaces can be been monitored using both plasmonic and fluorescent signatures. Our results demonstrate a novel bimodal imaging system that can be finely tuned with respect to nanoparticle size and protein concentration. Resulting hybrid probes may mitigate the limitation of depth penetration into biological tissues as well as providing high signal-to-noise ratio and sensitivity.

show abstract