Advances in Streptomyces coelicolor genetics.

Hopwood, David A.; Chater, Keith F.; Dowding, J E; Vivian, Alan

doi:10.1128/mmbr.37.3.371-405.1973

Cited by 128 publications

(47 citation statements)

References 60 publications

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“…S. coelicolor A3 [2] has the unusual ability to utilize agar as a sole carbon source [9][10][11] due to the expression of a gene, dagA, which encodes an extracellular agarase, the first enzyme in the pathway of agar catabolism [11]. dagA has been cloned [12,13] and its transcription pattern defined [14]; its transcription is initiated at four different promoters by at least three different RNA polymerase holoenzymes, which were identified by in vitro reconstitution experiments [15].…”

Section: Introductionmentioning

confidence: 99%

Heterologous recognition in vivo of promoter sequences from the Streptomyces coelicolor dagA gene

Parro¹

1993

FEMS Microbiology Letters

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The Streptomyces coelicolor dagA gene that codes for an extracellular agarase was cloned in the closely related bacterium S. lividans and transferred to the distantly related low G+C Gram-positive bacterium Bacillus subtilis and to the far more distantly related Gram-negative bacterium Escherichia coli. S1 nuclease mapping experiments identified a putative fifth promoter from which transcription of the dagA gene can take place, and accurately mapped the transcription termination site. The transcription terminator was specific for the Streptomyces strains and could terminate transcription initiated by promoters other than those of dagA. The agarase gene is efficiently transcribed in B. subtilis and E. coli, although pulse-chase experiments failed to detect the synthesis of agarase in these two bacteria.

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Section: Introductionmentioning

confidence: 99%

Heterologous recognition in vivo of promoter sequences from the Streptomyces coelicolor dagA gene

Parro¹

1993

FEMS Microbiology Letters

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“…Methods for mutation by ultraviolet light or nitrosoguanidine and the procedures for isolation and crossing of mutants were those already described for N. mediterranei [1] and almost identical to the methods developed for Streptomyces coelicolor [5,6].…”

Section: Genetic Methodsmentioning

confidence: 99%

Chromosomal mutations in the final step of rifamycin B biosynthesis

Schupp

1979

FEMS Microbiology Letters

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“…S. lividans strain TK21 [16], an agarase non-producing strain, was used as the host for the high copy number plasmid, pAGAs1, encoding dagA and thiostrepton resistance [30]. General procedures for the growth and manipulation of Streptomyces were as described previously [17].…”

Section: Bacterial Strains and Plasmidsmentioning

confidence: 99%

“…Chemostat cultures were inoculated with pre-germinated spore suspensions. High-density spore preparations (about 10 W spores/ml) were pre-germinated [16] and used to inoculate 900 ml of magnesium-limiting (phosphate replete) CHMM (30 mM K P SO R , 50 mM (NH R ) P SO R , 1 mM citric acid, 5 mM glutamic acid, 0.1 mM FeCl Q , 0.1 mM CaCl P , 37.5 WM MgCl P , 25 WM ZnCl P , 25 WM MnCl P , 5 WM CuCl P , 5 WM CoCl P , 5 WM Na Q MoO R , 5 WM phosphate NaH P PO R ) with 1% glucose or 2.5% mannitol as carbon sources in a 1 l fermentation vessel (New Brunswick BIO1 fermenter). For phosphate-limited growth, NaH P PO R was added to the medium at the lower concentration of 0.25 mM.…”

Section: Chemostat Culturesmentioning

confidence: 99%

“…In this paper we report the e¡ects of phosphate limitation on S. lividans strain TK21 containing an agarase gene (dagA) from S. coelicolor strain A3 (2). Agarase is the ¢rst enzyme in the pathway of agar catabolism [15] and allows S. coelicolor to utilise agar as a sole carbon source [15,16,35]. The dagA gene has been cloned [4,20,30] and its transcription pattern de¢ned [5,6,30].…”

Section: Introductionmentioning

confidence: 99%

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Effects of phosphate limitation on agarase production by Streptomyces lividansTK21

Parro¹

1998

FEMS Microbiology Letters

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The dagA gene from Streptomyces coelicolor, encoding an extracellular agarase, has been expressed in Streptomyces lividans on a multicopy plasmid. Chemostat cultures were used to investigate the influence of phosphate limitation on the synthesis and secretion of this enzyme. The strain was cultivated with glucose as the carbon source and magnesium or phosphate as growthlimiting substrates and samples used to determine dagA transcription, agarase secretion and alkaline phosphatase activity under the various limitations. The data show that, compared to growth under magnesium limitation, phosphate-limited cells show increases in dagA transcription, the rate of agarase production and secretion, and alkaline phosphatase activity. ß 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V.

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Advances in Streptomyces coelicolor genetics.

Cited by 128 publications

References 60 publications

Heterologous recognition in vivo of promoter sequences from the Streptomyces coelicolor dagA gene

Heterologous recognition in vivo of promoter sequences from the Streptomyces coelicolor dagA gene

Chromosomal mutations in the final step of rifamycin B biosynthesis

Effects of phosphate limitation on agarase production by Streptomyces lividansTK21

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