Advances in stem cell and other therapies for Huntington’s disease: An update

Conner, Lucy A.; Srinageshwar, Bhairavi; Jl, Bakke; Dunbar, Gary; Rossignol, Julien

doi:10.1016/j.brainresbull.2023.110673

Cited by 4 publications

(1 citation statement)

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“…As a method for a non-destructive imaging of whole brains ex vivo, however, this imaging protocol can be extremely useful. Future therapeutic visions might focus on transplants generated from mesenchymal stem cells and pluripotent stem cells to induce regenerative effects, to avoid logistic problems and ethics discussions inherent in the use of fetal tissue grafts [ 40 ]. Our protocol could be modified for use with those cell entities, rendering the combined approach of non-destructive three-dimensional imaging for morphological analysis at high spatial resolution and immunohistochemistry—to assess the function and integration of the transplants into the host environment—a very elegant analytic combination in the experimental workup.…”

Section: Discussionmentioning

confidence: 99%

3D Imaging of Striatal Transplants in a Small Animal Model of Huntington’s Disease

Schültke,

Pinzer,

Stampanoni

et al. 2023

Neurology International

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High-resolution imaging in small animal models of neurologic disease is a technical challenge. In a pilot project, we have explored a non-destructive synchrotron imaging technique for the 3D visualization of intracerebral tissue transplants in a well-established small animal model of Huntington’s disease. Four adult female Sprague Dawley rats each received injections of 0.12 M quinolinic acid (QA) into two target positions in the left striatum, thus creating unilateral left-sided striatal lesions similar to those frequently seen in patients suffering from Huntington’s disease. One week after lesioning, the animals received transplants prepared from whole ganglionic eminences (wGEs) obtained from 13- to 14-day-old rat embryos. Of the four lesioned animals, three received transplants of GNP-loaded cells and one animal received a transplant of naïve cells, serving as control. Post-mortem synchrotron-based microCT was used to obtain images of the neurotransplants. The images obtained of GNP-loaded tissue transplants at the synchrotron corresponded in size and shape to the histological images of transplants developed from naïve cells. Thus, we conclude that non-destructive synchrotron imaging techniques such as phase-contrast imaging are suitable to obtain high-resolution images of GNP-loaded tissue transplants.

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Section: Discussionmentioning

confidence: 99%