Advances in spray products for skin regeneration

Pleguezuelos-Beltrán, Paula; Gálvez‐Martín, Patricia; Nieto-García, Daniel; Marchal, Juan A.; López‐Ruiz, Elena

doi:10.1016/j.bioactmat.2022.02.023

Cited by 37 publications

(39 citation statements)

References 152 publications

(296 reference statements)

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“…This is because fibrin is a natural component regularly present in the human body [ 33 , 34 ]. In clinical practice, dermatologists usually use it to promote wound healing [ 35 , 36 ]. However, alginate originates from plants, and is an artificial component [ 37 , 38 ].…”

Section: Discussionmentioning

confidence: 99%

Automatic Evaluation for Bioengineering of Human Artificial Ovary: A Model for Fertility Preservation for Prepubertal Female Patients with a Malignant Tumor

Wang

Pei

Isachenko

et al. 2022

IJMS

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Introduction: The in vitro culture of primordial follicles is the only available option for preserving fertility in prepubertal girls with malignant tumors. The cultivation of primordial follicles in scaffolds as artificial ovaries is a promising approach for this. Methods: Dissociated follicles were placed into an artificial ovarian scaffold composed of fibrinogen and thrombin. The follicles were cultured in a dish dedicated to live cell imaging and observed for growth using immunofluorescence and development via optical microscopy. The morphology of the follicles in the scaffold was three-dimensionally reconstructed using the Imaris software. Growth and development were also quantified. Results: The morphology of artificial ovaries began to degrade over time. Within approximately 7 days, primordial follicles were activated and grew into secondary follicles. A comparison of optical and confocal microscopy results revealed the superior detection of live cells using confocal microscopy. The three-dimensional reconstruction of the confocal microscopy data enabled the automatic enumeration and evaluation of the overall morphology of many follicles. Conclusions: The novel artificial ovary-enabled primordial follicles to enter the growth cycle after activation and grow into secondary follicles. The use of a fibrin scaffold as a carrier preserves the developmental potential of primordial germ cells and is a potentially effective method for preserving fertility in prepubertal girls.

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Section: Discussionmentioning

confidence: 99%

Automatic Evaluation for Bioengineering of Human Artificial Ovary: A Model for Fertility Preservation for Prepubertal Female Patients with a Malignant Tumor

Wang

Pei

Isachenko

et al. 2022

IJMS

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“…Skin is considered as the largest organ and the main external defense system of the human body, which protects the human body from microbial infection and external environmental damage, and also plays an important role in regulating body temperature and sensing external stimuli [ [1] , [2] , [3] , [4] , [5] ]. However, owing to various factors including inflammation and infections, etc., the healing process of injured skin does not proceed in an orderly manner, which results in a long healing time [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Breathable, antifreezing, mechanically skin-like hydrogel textile wound dressings with dual antibacterial mechanisms

Jiang

Deng²,

Jin³

et al. 2023

Bioactive Materials

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“…This includes skin grafts or tissue flaps. In extensive cases, such as severe or extensive third-degree burns, therapeutic alternatives are needed due to a lack of uninjured autologous tissue [ 2 ]. The amplification of human keratinocyte cells by serial cultivation can provide large quantities of epidermal cells, which have the potential to restore the vital functions of the epidermis and dermis.…”

Section: Introductionmentioning

confidence: 99%

“…Different biomaterials, such as gelatin, chitosan, or collagen, have been used to culture and transplant human keratinocytes [ 12 , 13 , 14 ]. Encouraging results were obtained in several studies using a suspension of human keratinocytes in fibrin sealant, which represents an important biopolymer in the skin’s recovery process from large wounds [ 2 , 15 , 16 , 17 , 18 ]. It has been proven to be a suitable cell carrier that shows high tissue tolerance properties because it is completely resorbed [ 19 , 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

Air-Pressure-Supported Application of Cultured Human Keratinocytes in a Fibrin Sealant Suspension as a Potential Clinical Tool for Large-Scale Wounds

Sörgel

Schmid

Kengelbach‐Weigand

et al. 2022

JCM

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The treatment of large-scale skin wounds remains a therapeutic challenge. In most cases there is not enough autologous material available for full coverage. Cultured epithelial autografts are efficient in restoring the lost epidermal cover; however, they have some disadvantages, such as difficult application and protracted cell cultivation periods. Transplanting a sprayed keratinocyte suspension in fibrin sealant as biological carrier is an option to overcome those disadvantages. Here, we studied different seeding techniques regarding their applicability and advantages on cell survival, attachment, and outgrowth in vitro and thereby improve the cell transfer to the wound bed. Human primary keratinocytes were suspended in a fibrin sealant. WST-8 assay was used to evaluate the vitality for 7 days. Furthermore, the cells were labeled with CellTracker™ CM-Di-I and stained with a life/dead staining. Cell morphology, shape, and distribution were microscopically analyzed. There was a significant increase in vitality while cultivating the cells in fibrin. Sprayed cells were considerably more homogenously distributed. Sprayed cells reached the confluent state earlier than dripped cells. There was no difference in the vitality and morphology in both groups over the observation period. These findings indicate that the sprayed keratinocytes are superior to the application of the cells as droplets. The sprayed application may offer a promising therapeutic option in the treatment of large chronic wounds.

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Advances in spray products for skin regeneration

Cited by 37 publications

References 152 publications

Automatic Evaluation for Bioengineering of Human Artificial Ovary: A Model for Fertility Preservation for Prepubertal Female Patients with a Malignant Tumor

Automatic Evaluation for Bioengineering of Human Artificial Ovary: A Model for Fertility Preservation for Prepubertal Female Patients with a Malignant Tumor

Breathable, antifreezing, mechanically skin-like hydrogel textile wound dressings with dual antibacterial mechanisms

Air-Pressure-Supported Application of Cultured Human Keratinocytes in a Fibrin Sealant Suspension as a Potential Clinical Tool for Large-Scale Wounds

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