Advances in Somatic Embryogenesis Research of Horticultural Plants

Ji, Aiqing; Geng, X.-M.; Zhang, Yan; Wang, Guoliang

doi:10.4236/ajps.2011.26087

Cited by 26 publications

(8 citation statements)

References 19 publications

(33 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The efficiency of the leaf, shoot tip, and ray floret explants of the chrysanthemum for the direct in vitro regeneration were compared in a survey and shoot tips were reported to be the most suitable types of explants (19). To emphasize more, it should be acknowledged that different PGRs show dissimilar peculiarity and requirements for these hormones differs amongst various explants (20). Furthermore, protocols suitable for a given cultivar are not necessarily efficient for the others (17).…”

Section: Introductionmentioning

confidence: 99%

Factors Influencing in Vitro Organogenesis of Chrysanthemum morifolium cv. ‘Resomee Splendid’

et al. 2018

View full text Add to dashboard Cite

BackgroundChrysanthemum; also commonly known as mums or chrysanths, is one of the most important ornamental crops worldwide. Introducing desirable traits into this valuable plant by the conventional breeding has so far been faced with some restrictions due to the limited gene pool and cross-incompatibility. Therefore, breeders have decided to exploit Agrobacterium-mediated transformation methods in order to satisfy the growing market demands. However, more efficient in vitro regeneration protocols are required for this approach.ObjectivesThe objective of this research was to develop an efficient protocol for an in vitro plant regeneration by the examining the effects of various combinations and concentrations of the plant growth regulators (PGRs) and different explants types.Materials and MethodsThe leaf and petiole explants of the Chrysanthemum morifolium cv. ‘Resomee Splendid’ were collected from the in vitro grown plantlets. Murashige and Skooge (MS) medium was supplemented with different concentrations and combinations of benzylaminopurine (BAP), 1-naphthaleneacetic acid (NAA) and thidiazuron (TDZ). Thereafter, the effects of these hormonal treatments were investigated on shoot initiation percentage, the average number of shoots per explants, callogenesis, and the type of organogenesis in regard to both types of the explants.ResultsShoots were directly formed from leaf explants on the media that only contained BAP without callus formation. Amongst the other hormonal treatments, a combination of 4.5 mg.L-1 BAP plus 1 mg.L-1 NAA resulted in the direct organogenesis from the leaf explants, which was superior to the other combinations and concentrations. In regard to the petiole explants, direct shoot formation occurred in all the media except for the ones which were fortified with TDZ. In this case, considering the shoot initiation percentage and the mean shoot number per explants, the best results were achieved in the medium supplemented with 1.5 mg.L-1 BAP and 1 mg.L-1 NAA. Results showed that interaction of either BAP or TDZ with NAA was necessary for the callus induction.ConclusionsSignificant differences in shoot initiation percentage and the average number of shoots per explants were observed both in leaves and petioles grown on different media. Moreover, the callogenesis rates, as well as organogenesis types, showed some differences among the studied explants when compared on the same media.

show abstract

Section: Introductionmentioning

confidence: 99%

Factors Influencing in Vitro Organogenesis of Chrysanthemum morifolium cv. ‘Resomee Splendid’

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Concerning the choice of the source of explant used to obtain peach somatic embryos, various aspects must be considered. In general, the age of tissue has an impact on SE in horticultural plants [ 49 ]. The endogenous hormonal balance of P. persica cotyledons influenced their capacity to pass through from the differentiated to the embryogenic stage [ 50 ].…”

Section: Further Approaches Applied To Improve Peach Regeneration-mentioning

confidence: 99%

“…The endogenous hormonal balance of P. persica cotyledons influenced their capacity to pass through from the differentiated to the embryogenic stage [ 50 ]. Ji et al [ 49 ] remarked that a young tissue at early stages of development, with a high level of basal metabolism, seems to be more susceptible for SE induction compared to an older differentiated tissue. In fact, SE induction can occur only if a differentiated cell regains its totipotency [ 51 ].…”

Section: Further Approaches Applied To Improve Peach Regeneration-mentioning

confidence: 99%

Genetic Transformation in Peach (Prunus persica L.): Challenges and Ways Forward

Ricci

Sabbadini

Prieto

et al. 2020

Plants

View full text Add to dashboard Cite

Almost 30 years have passed since the first publication reporting regeneration of transformed peach plants. Nevertheless, the general applicability of genetic transformation of this species has not yet been established. Many strategies have been tested in order to obtain an efficient peach transformation system. Despite the amount of time and the efforts invested, the lack of success has significantly limited the utility of peach as a model genetic system for trees, despite its relatively short generation time; small, high-quality genome; and well-studied genetic resources. Additionally, the absence of efficient genetic transformation protocols precludes the application of many biotechnological tools in peach breeding programs. In this review, we provide an overview of research on regeneration and genetic transformation in this species and summarize novel strategies and procedures aimed at producing transgenic peaches. Promising future approaches to develop a robust peach transformation system are discussed, focusing on the main bottlenecks to success including the low efficiency of A. tumefaciens-mediated transformation, the low level of correspondence between cells competent for transformation and those that have regenerative competence, and the high rate of chimerism in the few shoots that are produced following transformation.

show abstract

“…Somatic embryogenesis is a technique in which the somatic cells of a mother plant develop into a whole new plant [5]. The whole process of somatic embryogenesis is very similar with zygotic embryogenesis [5], and the morphology of somatic embryos is similar to that of zygotic embryos [6,7]. It is a well-received technology for mass multiplication due to its high multiplication efficiency and short production time [8,9].…”

Section: Introductionmentioning

confidence: 99%

Enhanced Somatic Embryo Induction of a Tree Peony, Paeonia ostii ‘Fengdan’, by a Combination of 6-benzylaminopurine (BA) and 1-naphthylacetic Acid (NAA)

Ren

Jeong

2019

Plants

View full text Add to dashboard Cite

Somatic embryogenesis is a preferred method for vegetative propagation due to its high propagation efficiency. In this study, zygotic embryos, cotyledons, and hypocotyls of Paeonia ostii ‘Fengdan’ were used as the explant to induce somatic embryogenesis. The results showed that a combination of 0.5 mg·L−1 thidiazuron (TDZ) and 0.5 mg·L−1 2,4-dichlorophenoxyacetic acid (2,4-D) was effective in inducing somatic embryos from the zygotic embryo and cotyledon explants. Hypocotyls only formed somatic embryos on Murashige and Skoog (MS) medium supplemented with both 0.5 mg·L−1 TDZ and 0.5 mg·L−1 1-naphthylacetic acid (NAA). Moreover, the compact callus was effectively produced from zygotic embryo, cotyledon, and hypocotyl explants in medium supplemented with a combination of 3.0 mg·L−1 6-benzylaminopurine (BA) and 1.0 mg·L−1 NAA, and then converted into somatic embryos in the same medium, and the ratio of the explants with embryo induction and number of embryos induced per explant were much higher than those induced by 0.5 mg·L−1 TDZ and either 0.5 mg·L−1 2,4-D or 0.5 mg·L−1 NAA. The MS medium was better than the woody plant medium (WPM) for inducing somatic embryos from zygotic embryo and hypocotyl explants, whereas the WPM was better than the MS medium for somatic embryogenesis induction from cotyledon explants. All of the somatic embryos developed well into mature embryos on their respective media supplemented with both 3.0 mg·L−1 BA and 1.0 mg·L−1 NAA. Overall, the protocols for indirect somatic embryogenesis from zygotic embryo, cotyledon, and hypocotyl of P. ostii ‘Fengdan’ were successfully established, which can greatly facilitate their propagation and breeding processes.

show abstract

Advances in Somatic Embryogenesis Research of Horticultural Plants

Abstract: ABSTRACT

Cited by 26 publications

References 19 publications

Factors Influencing in Vitro Organogenesis of Chrysanthemum morifolium cv. ‘Resomee Splendid’

Factors Influencing in Vitro Organogenesis of Chrysanthemum morifolium cv. ‘Resomee Splendid’

Genetic Transformation in Peach (Prunus persica L.): Challenges and Ways Forward

Enhanced Somatic Embryo Induction of a Tree Peony, Paeonia ostii ‘Fengdan’, by a Combination of 6-benzylaminopurine (BA) and 1-naphthylacetic Acid (NAA)

Contact Info

Product

Resources

About