Advances in screening hyperthermic nanomedicines in 3D tumor models

Soeiro, Joana F.; Sousa, Filipa L.; Monteiro, Maria V.; Gaspar, Vítor M.; Silva, Nuno J. O.; Mano, João F.

doi:10.1039/d3nh00305a

Cited by 5 publications

(4 citation statements)

References 317 publications

(462 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The key advantages of 3D in vitro cultures compared to classical 2D models in adherent culture vessels is that they provide more physiologically relevant responses for nanoparticle–cell interaction assessments, especially regarding tissue penetration and internalization [ 151 , 152 , 153 , 154 , 155 ]. Cells benefit from a 3D architecture due to the organization of the ECM in a manner closer to physiological conditions [ 156 , 157 ].…”

Section: Immunohistological Studies For Complex Biological Structuresmentioning

confidence: 99%

“…They are applicable for quantitative assessments of cell viability following interactions with NPs, methods that require a large number of spheroids to promote statistically reliable results. The key advantages of 3D in vitro cultures compared to classical 2D models in adherent culture vessels is that they provide more physiologically relevant responses for nanoparticle-cell interaction assessments, especially regarding tissue penetration and internalization [151][152][153][154][155]. Cells benefit from a 3D architecture due to the organization of the ECM in a manner closer to physiological conditions [156,157].…”

Section: Immunohistological Studies For Complex Biological Structuresmentioning

confidence: 99%

“…This organization promotes multicellular heterogeneity, not only in monocellular but also in multicellular cultures. By cultivating cells in a 3D architecture, a physiological gradient of oxygen, nutrients, and signaling mol- The key advantages of 3D in vitro cultures compared to classical 2D models in adherent culture vessels is that they provide more physiologically relevant responses for nanoparticle-cell interaction assessments, especially regarding tissue penetration and internalization [151][152][153][154][155]. Cells benefit from a 3D architecture due to the organization of the ECM in a manner closer to physiological conditions [156,157].…”

Section: Immunohistological Studies For Complex Biological Structuresmentioning

confidence: 99%

See 2 more Smart Citations

Unveiling Nanoparticles: Recent Approaches in Studying the Internalization Pattern of Iron Oxide Nanoparticles in Mono- and Multicellular Biological Structures

Petcov,

Straticiuc,

Iancu

et al. 2024

JFB

View full text Add to dashboard Cite

Nanoparticle (NP)-based solutions for oncotherapy promise an improved efficiency of the anticancer response, as well as higher comfort for the patient. The current advancements in cancer treatment based on nanotechnology exploit the ability of these systems to pass biological barriers to target the tumor cell, as well as tumor cell organelles. In particular, iron oxide NPs are being clinically employed in oncological management due to this ability. When designing an efficient anti-cancer therapy based on NPs, it is important to know and to modulate the phenomena which take place during the interaction of the NPs with the tumor cells, as well as the normal tissues. In this regard, our review is focused on highlighting different approaches to studying the internalization patterns of iron oxide NPs in simple and complex 2D and 3D in vitro cell models, as well as in living tissues, in order to investigate the functionality of an NP-based treatment.

show abstract

Section: Immunohistological Studies For Complex Biological Structuresmentioning

confidence: 99%

Section: Immunohistological Studies For Complex Biological Structuresmentioning

confidence: 99%

Section: Immunohistological Studies For Complex Biological Structuresmentioning

confidence: 99%

See 1 more Smart Citation

Unveiling Nanoparticles: Recent Approaches in Studying the Internalization Pattern of Iron Oxide Nanoparticles in Mono- and Multicellular Biological Structures

Petcov,

Straticiuc,

Iancu

et al. 2024

JFB

View full text Add to dashboard Cite

show abstract

“…In today’s fast-paced world of life science and biomedical research, a large body of experimental evidence has clearly demonstrated the enormous potential of three-dimensional (3D) cell cultures for improving our understanding of cell biology and the molecular mechanisms underlying disease [ 1 , 2 , 3 ], for drugs development and testing [ 4 , 5 , 6 , 7 ], in regenerative medicine [ 8 , 9 ] and also in tissue engineering [ 1 ]. Research has focused heavily on developing protocols and fine-tuning new technological approaches to developing diverse 3D in vitro models, also in agreement with the 3Rs (Replacement, Reduction, Refinement) principles of the European Union [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Growing Role of 3D In Vitro Cell Cultures in the Study of Cellular and Molecular Mechanisms: Short Focus on Breast Cancer, Endometriosis, Liver and Infectious Diseases

Bloise,

Giannaccari,

Guagliano

et al. 2024

Cells

View full text Add to dashboard Cite

Over the past decade, the development of three-dimensional (3D) models has increased exponentially, facilitating the unravelling of fundamental and essential cellular mechanisms by which cells communicate with each other, assemble into tissues and organs and respond to biochemical and biophysical stimuli under both physiological and pathological conditions. This section presents a concise overview of the most recent updates on the significant contribution of different types of 3D cell cultures including spheroids, organoids and organ-on-chip and bio-printed tissues in advancing our understanding of cellular and molecular mechanisms. The case studies presented include the 3D cultures of breast cancer (BC), endometriosis, the liver microenvironment and infections. In BC, the establishment of 3D culture models has permitted the visualization of the role of cancer-associated fibroblasts in the delivery of exosomes, as well as the significance of the physical properties of the extracellular matrix in promoting cell proliferation and invasion. This approach has also become a valuable tool in gaining insight into general and specific mechanisms of drug resistance. Given the considerable heterogeneity of endometriosis, 3D models offer a more accurate representation of the in vivo microenvironment, thereby facilitating the identification and translation of novel targeted therapeutic strategies. The advantages provided by 3D models of the hepatic environment, in conjunction with the high throughput characterizing various platforms, have enabled the elucidation of complex molecular mechanisms underlying various threatening hepatic diseases. A limited number of 3D models for gut and skin infections have been developed. However, a more profound comprehension of the spatial and temporal interactions between microbes, the host and their environment may facilitate the advancement of in vitro, ex vivo and in vivo disease models. Additionally, it may pave the way for the development of novel therapeutic approaches in diverse research fields. The interested reader will also find concluding remarks on the challenges and prospects of using 3D cell cultures for discovering cellular and molecular mechanisms in the research areas covered in this review.

show abstract

An Overview of Advancements and Technologies in Vascularization Strategies for Tumor‐On‐A‐Chip Models

Parihar,

Sunildutt,

Rahim

et al. 2024

Advanced Therapeutics

View full text Add to dashboard Cite

Vascularized tumor on a chip (VToC) entails emulating intricate microvascular networks like those observed in tumors through microfluidic devices, which are meticulously designed to offer a faithful representation of cancer in vitro, exploration of tumor biology, evaluation of drug efficacy, and anticipation of patient responses to therapies. Compared to conventional ones, VToC systems hold advantages by creating a milieu where physiological conditions for investigating tumor‐host interactions are pivotal in tumor advancement/therapy resilience. Nevertheless, VToC models confront limitations encompassing vascular network replication, biological fidelity, mechanical/chemical integrity, and intricacies of architectural design. Thus, drawbacks inherent to prevailing VToC models' intricacies, attributes, and vascular network establishment are imperative. This systematic review focuses on the recent advancements, technologies explored for incorporating VToC models, & vascularization approaches for investigation, and factors/parameters affecting complex tumor microenvironments in VToC models, along with the futuristic approach for the design strategies, fabrication techniques, understanding of vascular network, also VToC models with spheroid. A comprehensive analysis of VToC based on their limitations for a practical approach highlights the promising strategies for possible applications. This review will be essential regarding a complete overview of VToC models and the future direction toward developing efficient VToC models compared to the state‐of‐the‐art VToC.This article is protected by copyright. All rights reserved

show abstract

Advances in screening hyperthermic nanomedicines in 3D tumor models

Abstract: Hyperthermia nanotherapeutics are particularly relevant for tackling complex human cancers. The early-stage performance evaluation of innovative hyperthermic anti-cancer nanomedicines is conventionally performed in flat 2D cell cultures that do not...

Cited by 5 publications

References 317 publications

Unveiling Nanoparticles: Recent Approaches in Studying the Internalization Pattern of Iron Oxide Nanoparticles in Mono- and Multicellular Biological Structures

Unveiling Nanoparticles: Recent Approaches in Studying the Internalization Pattern of Iron Oxide Nanoparticles in Mono- and Multicellular Biological Structures

Growing Role of 3D In Vitro Cell Cultures in the Study of Cellular and Molecular Mechanisms: Short Focus on Breast Cancer, Endometriosis, Liver and Infectious Diseases

An Overview of Advancements and Technologies in Vascularization Strategies for Tumor‐On‐A‐Chip Models

Contact Info

Product

Resources

About