Advances in salt tolerance molecular mechanism in tobacco plants

Sun, Haijie; Sun, Xiaowen; Wang, Hui

doi:10.1186/s41065-020-00118-0

Cited by 24 publications

(14 citation statements)

References 89 publications

(88 reference statements)

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“…Tobacco plants (Nicotiana tabacum L.) have already begun to be studied as environmentally friendly, as they are tolerant to abiotic stresses and have the genetic potential for bioremoval of salts and metals ions from soils (Sun et al, 2020). Despite the ability to accumulate metals, tobacco plants were adversely affected by vanadium.…”

Section: Discussionmentioning

confidence: 99%

“…The use of plants which are resistant to various abiotic stresses (drought, high and low temperatures) in phytoremediation processes may be especially promising for the development of effective environmental biotechnologies. For this purpose, it is promising to use tobacco plants (Nicotiana tabacum L.), which have recently been studied for their resistance to various abiotic factors (Sun et al, 2020). Tobacco plants are resistant to high concentrations of salts and also have molecular genetic determinants of active transport of minerals into plant tissues (Sun et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

“…For this purpose, it is promising to use tobacco plants (Nicotiana tabacum L.), which have recently been studied for their resistance to various abiotic factors (Sun et al, 2020). Tobacco plants are resistant to high concentrations of salts and also have molecular genetic determinants of active transport of minerals into plant tissues (Sun et al, 2020). Given their resistance to stress and the ability to accumulate a variety of ions, N. tabacum plants are highly promising for phytobioremediation of ecosystems.…”

Section: Introductionmentioning

confidence: 99%

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Bioremoval of hazardous cobalt, nickel, chromium, copper and cadmium compounds from contaminated soil by Nicotiana tabacum plants and associated microbiome

et al. 2021

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Contamination of soils with heavy metals leads to reduction of soil fertility, destruction of natural ecosystems and detrimental effects on the health of society by increasing content of metals in the food chains from microorganisms to plants, animals and humans. Bioremediation is one of the most promising and cost-effective methods of cleaning soils polluted with toxic metals. According to current researchers, microorganisms and plants have the genetic potential to remove toxic metals from contaminated sites. The method of thermodynamic prediction was used to theoretically substantiate the mechanisms of interaction of soil microorganisms and plants with heavy metals. According to the our prediction, exometabolite chelators of anaerobic microorganisms may increase the mobility of metals and thereby contribute to the active transport of metals and their accumulation in plants. Plants of Nicotiana tabacum L. of Djubek cultivar were used as plant material for the current investigation. The examined toxicants were heavy metals, namely cobalt (II), nickel (II), chromium (VI), copper (II) and cadmium (II). The aqueous solutions of metal salts were added to the boxes after two months of plants growing to the final super-high concentration – 500 mg/kg of absolutely dry weight of soil. Quantitative assessments of copper and chromium-resistant microorganisms were made by cultivation on agar nutrient medium NA with a gradient of Cu(II) and Cr(VI). The concentration of metals in soil and plant material (leaves, stems and roots) was determined by atomic absorption method. The study revealed that heavy metals inhibited the growth of the examined tobacco plants. This was expressed by the necrosis of plant tissues and, ultimately, their complete death. Despite this, all investigated heavy metals were accumulated in plant tissues during 3–7 days before death of plants. The uptake of metals was observed in all parts of plants – leaves, stems and roots. The highest concentrations of Co(II), Ni(II), Cd(II), Cr(VI) were found in the leaves, Cu(II) – in the roots. The results show that the bioremoval efficiency of the investigated metals ranged 0.60–3.65%. Given the super-high initial concentration of each of the metals (500 mg/kg), the determined removal efficiency was also high. Cadmium was the most toxic to plants. Thus, the basic points of the thermodynamic prognosis of the possibility of accumulation of heavy metals by phytomicrobial consortium were experimentally confirmed on the example of N. tabacum plants and metal-resistant microorganisms. The study demonstrated that despite the high initial metals concentration, rate of damage and death of plants, metals are accumulated inplant tissues in extremely hight concentrations. Soil microorganisms were observed to have high adaptation potencial to Cu(II) and Cr(VI). In anaerobic conditions, microorganisms presumably mobilize heavy metals, which later are absorbed by plants. The obtained results are the basis for the development of environmental biotechnologies for cleaning contaminated soils from heavy metal compounds.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Bioremoval of hazardous cobalt, nickel, chromium, copper and cadmium compounds from contaminated soil by Nicotiana tabacum plants and associated microbiome

et al. 2021

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“…The tobacco plant exhibits higher seed germination and survival rates, while the lettuce plant is widely consumed across the globe and is a very attractive expression system 89, 90 . The chloroplasts of these plants are used to synthesize proinsulin.…”

Section: Tobacco and Lettuce Plantsmentioning

confidence: 99%

Human Insulin: History, Recent Advances, and Expression Systems for Mass Production

et al. 2021

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The significant rise in the number of diabetic patients worldwide, as well as the development of new insulin delivery techniques such as inhalation or oral administration which require higher dosages, are expected to increase the demand for recombinant insulin. Current manufacturing technologies will be unable to fulfill the rising demand for inexpensive insulin due to their production capacity limitations and high production costs. Production of therapeutic recombinant insulin requires a suitable host organism with adequate post-translational modification and refolding machinery. E. coli and S. cerevisiae have been used extensively to make recombinant human insulin for medicinal applications. However, transgenic plants are particularly appealing expression systems as they can be used to synthesize huge amounts of insulin for human medicinal purposes. Plant-based expression systems have the potential for high-capacity insulin synthesis at a minimal cost. The significant production of biologically active proinsulin in seeds or leaves with long-term stability provides a low-cost technique to develop proinsulin for both injectable and oral administration. Recently, stem cell therapy is being utilized for the treatment of diabetes, as these cells are capable of differentiating into insulin producing c ells. With the advancement of regenerative medicine research for different chronic diseases, treatment for type 1 diabetes mellitus has been reported. The current review concentrates on several biotechnological attributes applied to the rapid and mass synthesis of biologically active insulin and its analogs in microbes, various types of stem cells and transgenic crops.

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“…The physiology and genetics of the Nicotiana genus has been widely explored for example its ability to regenerate into a specilised tissues to form a fully grown living organism from its de-differentiated cells. Nicotiana tabacum was always selected for developing of genetically modified plant model having functional of foreign genetic materials [22,23,24,25,26,49].…”

Section: Introductionmentioning

confidence: 99%

Plant tissue culture of Nicotiana tabacum cv. TAPM 26 and its minimum inhibition against herbicide-Dalapon

Taalat

Javed

Huyop

et al. 2021

MANAS Journal of Engineering

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Current study is to establish a basic plant tissue culture of Nicotiana tabacum TAPM 26 and test the plant tissue on resistancies against 2,2 DCP an active ingredient in herbicide-Dalapon. During micropropagation, the surface sterilization method was ascertained on seeds of tobacco. HgCl2 was used to disinfect tobacco seeds at different concentrations (0.05 gL -1 , 0.2 gL -1 , 0.5 gL -1 and 1.0 gL -1 ) within three minutes. About 70% seeds were survived when exposed to 0.05 gL -1 of HgCl2, whereas, no seeds were germinated when sterilized at concentrations above 0.05 gL -1 of HgCl2. To optimize an efficient protocol of shoots and callus formation during in vitro regeneration, explant types and plant growth were studied. Growth regulators NAA (0.1 mgL -1 , 0.2 mgL -1 , 0.5 mgL -1 , 1.0 mgL -1 and 2.0 mgL -1 ) and BAP (1.0 mgL -1 , 2.0 mgL -1 , 3.0 mgL -1 and 4.0 mgL -1 ) were used. The explants types were one month old leaves and two weeks old cotyledons. The maximum numbers of shoots per explants were obtained from cotyledon with combination 0.1 mgL -1 NAA and 1.0 mgL -1 BAP. The highest callus fresh weight was achieved when NAA 0.5 mgL -1 with BAP 1.0 mgL -1 after four weeks. Thus, the highest number of shoots produced per explants from leaves culture on the MS media containing 0.2 mgL -1 NAA and 4.0 mgL -1 BAP. The best callus fresh weight was obtained with combination of 1.0 mgL -1 NAA and 1.0 mgL -1 BAP by using leaves explant. Finally, Dalapon (5 gL -1 , 10 gL -1 , 15 gL -1 and 20 gL -1 ) were applied onto leaves and cotyledon cultures of N. tabacum to check on the minimum concentration of inhibition. The minimum concentration of inhibition of leaves and cotyledon cultures of N. tabacum was at 5 gL -1 of 2,2DCP but not at 10 gL -1 , 15 gL -1 and 20 gL -1 . This investigation will shed alight for future studies on transgenic tobacco resistant against Dalapon Research article

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Advances in salt tolerance molecular mechanism in tobacco plants

Cited by 24 publications

References 89 publications

Bioremoval of hazardous cobalt, nickel, chromium, copper and cadmium compounds from contaminated soil by Nicotiana tabacum plants and associated microbiome

Bioremoval of hazardous cobalt, nickel, chromium, copper and cadmium compounds from contaminated soil by Nicotiana tabacum plants and associated microbiome

Human Insulin: History, Recent Advances, and Expression Systems for Mass Production

Plant tissue culture of Nicotiana tabacum cv. TAPM 26 and its minimum inhibition against herbicide-Dalapon

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