Advances in renewable plant-derived protein source: The structure, physicochemical properties affected by ultrasonication

Enzymolysis of walnut ( Juglans regia L.) meal protein: Ultrasonication‐assisted alkaline pretreatment impact on kinetics and thermodynamics

“…A single frequency (40 kHz) of a Multi‐Frequency Counter Current S‐Type ultrasound (MFSU) machine (WKS 300/6S, Jiangsu Jiangda Wukesong Biotechnology Co. Ltd., China) equipped with five frequencies developed recently by our research group was employed in the experiment. The schematic diagram (Figure a) and the actual optical image (Figure b) (Wen, Zhang, Yao, et al, ) are herein represented. The device is classified under the low‐frequency (kHz range) ultrasound devices (Ma et al, ).…”

“…A modified version of Wen, Zhang, Yao, et al () protocol was employed for the intrinsic fluorescence spectra assay. Ten milligrams of freeze‐dried walnut meal protein powder was dissolved in 0.01 mol/l phosphate buffer saline (pH 8.0), and mixed thoroughly by agitating in a desktop constant temperature oscillator IS‐RDD3 (Mafu experimental equipment Co., Ltd, Shanghai, China) (30 min, 38°C, 240 rpm) to achieve a final concentration of 1.0 mg/ml.…”

“…The well-dried and pulverized (ground) walnut meal was screened with 60-micron mesh to obtain walnut meal flour. A preliminary study on the optimization process for the ultrasound-alkaline as- Figure 1b) (Wen, Zhang, Yao, et al, 2018) are herein represented. The device is classified under the low-frequency (kHz range) ultrasound devices .…”

“…F I G U R E 1 Pictorial representation of (a) Schematic diagram and (b) (i) Actual optical image (Wen, Zhang, Yao, et al, 2018) (ii) Experimental setup of the Multi-frequency counter current s-type (MFSU) ultrasound machine employed for the ultrasound-assisted alkaline pretreatment of the walnut meal for protein extraction where C 0 represents the initial WMP concentrations; V 0 (g l −1 min −1 )…”

Antioxidant Peptide Fractions Isolated from Wheat Germ Protein with Subcritical Water Extraction and Its Transport Across Caco‐2 Cells

“…However, this method has some disadvantages including long extraction time, high energy-consumption, and poor efficiency, among others . To circumvent these problems, some new technologies such as subcritical water extraction (SWE) (Erşan,ÖG, Carle, & Schweiggert, 2018;Zhang et al, 2018a;Zhang et al, 2019a), ultrasonic-assisted extraction (UE) (Wen et al, 2018a;Wen et al, 2018b;Wen et al, 2019a), high pressure-assisted extraction (HPE) (Alexandre et al, 2019;Sun et al, 2018;Zhang, Wen, Duan, Zhang, & Ma, 2019c), and microwave-assisted extraction (MAE) (Chuyen, Nguyen, Roach, Golding, & Parks, 2018;Leão, Botelho, Oliveira, & Franca, 2018) have been used to obtain bioactive compounds. Especially, SWE has attracted more and more interests due to its high extraction rates, environmentally friendly, and low organic solvent consump- JFDS-2019-0378 Submitted 3/16/2019, Accepted 5/29/2019 tion, et al (Ayala & De Castro, 2001;Ibanez et al, 2003;Zhang et al, 2019b).…”