Advances in PET and MRI imaging of tumor hypoxia

Gouel, Pierrick; Decazes, Pierre; Véra, Pierre; Gardin, Isabelle; Thureau, S.; Bohn, Pierre

doi:10.3389/fmed.2023.1055062

Cited by 13 publications

(5 citation statements)

References 197 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, the ability of purely induced hypoxic conditions to accurately simulate the in vivo microenvironment state remains to be discussed. The spatial distribution of hypoxia within tumors is usually significantly heterogeneous, often changing over time, and significant changes in oxygenation status may even occur between several cell layers [35][36][37]. Therefore, the focus of future work may lie in endogenous markers of hypoxia within tumors, using these markers to select more pure hypoxic cells for further experiments.…”

Section: Discussionmentioning

confidence: 99%

DKK2 promotes the progression of oral squamous cell carcinoma through the PI3K/AKT signaling pathway

Guo,

Qu,

et al. 2024

Aging

View full text Add to dashboard Cite

Objective: This study aimed to investigate the impact of Dickkopf 2 (DKK2) on the progression of oral squamous cell carcinoma (OSCC) and explore its role in the PI3K/AKT signaling transduction pathway. Materials and Methods: The study initially examined the expression of the DKK2 gene in OSCC tissues and normal tissues. Simultaneously, the expression of DKK2 in HOK cells and OSCC cells was verified, and changes in DKK2 expression under hypoxic conditions were detected. DKK2 overexpression and knockdown were performed in SCC-15 and CAL-27 cells. Subsequently, the effects of DKK2 on the proliferation, migration and invasion of OSCC were detected. Western blotting was employed to detect the expression of key proteins in the DKK2/PI3K/AKT signaling axis before and after transfection, and further explore the relevant molecular mechanisms. Results: Compared to normal tissues, DKK2 expression was elevated in OSCC tissues. The expression of DKK2 in the SCC-15 and CAL-27 cell lines was higher than that in HOK cells, and hypoxic conditions could promote DKK2 expression. DKK2 overexpression promoted cell proliferation, migration, and invasion, while DKK2 knockdown inhibited these processes. DKK2 overexpression activated the PI3K/AKT pathway, while DKK2 knockdown suppressed this pathway. Conclusion: This study suggests that hypoxic conditions enhance the expression of DKK2 in OSCC. DKK2 regulates the proliferation, migration, and invasion of OSCC through the PI3K/AKT signaling pathway.

show abstract

Section: Discussionmentioning

confidence: 99%

DKK2 promotes the progression of oral squamous cell carcinoma through the PI3K/AKT signaling pathway

Guo,

Qu,

et al. 2024

Aging

View full text Add to dashboard Cite

show abstract

“…Several PET tracers to assess tumor hypoxia are currently being investigated in both preclinical and clinical studies. However, so far no optimal tracer to assess cellular hypoxia has been established ( 83 ).…”

Section: The Tumor Microenvironment (Tme)mentioning

confidence: 99%

The development process of ‘fit-for-purpose’ imaging biomarkers to characterize the tumor microenvironment

Eertink,

Bahce,

Waterton

et al. 2024

Front. Med.

View full text Add to dashboard Cite

Immune-based treatment approaches are successfully used for the treatment of patients with cancer. While such therapies can be highly effective, many patients fail to benefit. To provide optimal therapy choices and to predict treatment responses, reliable biomarkers for the assessment of immune features in patients with cancer are of significant importance. Biomarkers (BM) that enable a comprehensive and repeatable assessment of the tumor microenvironment (TME), the lymphoid system, and the dynamics induced by drug treatment can fill this gap. Medical imaging, notably positron emission tomography (PET) and magnetic resonance imaging (MRI), providing whole-body imaging BMs, might deliver such BMs. However, those imaging BMs must be well characterized as being ‘fit for purpose’ for the intended use. This review provides an overview of the key steps involved in the development of ‘fit-for-purpose’ imaging BMs applicable in drug development, with a specific focus on pharmacodynamic biomarkers for assessing the TME and its modulation by immunotherapy. The importance of the qualification of imaging BMs according to their context of use (COU) as defined by the Food and Drug Administration (FDA) and National Institutes of Health Biomarkers, EndpointS, and other Tools (BEST) glossary is highlighted. We elaborate on how an imaging BM qualification for a specific COU can be achieved.

show abstract

“…For TPLA, Magnetic Resonance Imaging (MRI) guidance offers several advantages over guidance from other imaging modalities such as ultrasound, including superior soft tissue contrast for visualizing tumors and real-time temperature monitoring [ 9 – 12 ]. These advantages motivate the development of new Magnetic Resonance (MR) safe or MR conditional needles.…”

Section: Introductionmentioning

confidence: 99%

Design and evaluation of a pneumatic actuation unit for a wasp-inspired self-propelled needle

Bloemberg,

Hoppener,

Coolen

et al. 2024

PLoS ONE

View full text Add to dashboard Cite

Transperineal laser ablation is a minimally invasive thermo-ablative treatment for prostate cancer that requires the insertion of a needle for accurate optical fiber positioning. Needle insertion in soft tissues may cause tissue motion and deformation, resulting in tissue damage and needle positioning errors. In this study, we present a wasp-inspired self-propelled needle that uses pneumatic actuation to move forward with zero external push force, thus avoiding large tissue motion and deformation. The needle consists of six parallel 0.25-mm diameter Nitinol rods driven by a pneumatic actuation system. The pneumatic actuation system consists of Magnetic Resonance (MR) safe 3D-printed parts and off-the-shelf plastic screws. A self-propelled motion is achieved by advancing the needle segments one by one, followed by retracting them simultaneously. The advancing needle segment has to overcome a cutting and friction force, while the stationary needle segments experience a friction force in the opposite direction. The needle self-propels through the tissue when the friction force of the five stationary needle segments overcomes the sum of the friction and cutting forces of the advancing needle segment. We evaluated the prototype’s performance in 10-wt% gelatin phantoms and ex vivo porcine liver tissue inside a preclinical Magnetic Resonance Imaging (MRI) scanner in terms of the slip ratio of the needle with respect to the phantom or liver tissue. Our results demonstrated that the needle was able to self-propel through the phantom and liver tissue with slip ratios of 0.912–0.955 and 0.88, respectively. The prototype is a promising step toward the development of self-propelled needles for MRI-guided transperineal laser ablation as a method to treat prostate cancer.

show abstract

Advances in PET and MRI imaging of tumor hypoxia

Cited by 13 publications

References 197 publications

DKK2 promotes the progression of oral squamous cell carcinoma through the PI3K/AKT signaling pathway

DKK2 promotes the progression of oral squamous cell carcinoma through the PI3K/AKT signaling pathway

The development process of ‘fit-for-purpose’ imaging biomarkers to characterize the tumor microenvironment

Design and evaluation of a pneumatic actuation unit for a wasp-inspired self-propelled needle

Contact Info

Product

Resources

About