Advances in organic polymer-based monolithic column technology for high-resolution liquid chromatography-mass spectrometry profiling of antibodies, intact proteins, oligonucleotides, and peptides

Eeltink, Sebastiaan; Wouters, Sam; Dores-Sousa, José Luís; Švec, František

doi:10.1016/j.chroma.2017.01.002

Cited by 74 publications

(54 citation statements)

References 135 publications

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“…Originally, monolithic materials were tuned towards high permeability, allowing the use of long column formats for high plate‐number separations. To outperform conventional packed columns, much effort has been directed to tune both the feature sizes (globule/skeleton size and the diameter of the macropores) while maximizing the porosity . Motokawa et al.…”

Section: Advancing Performance Limits and Selected Examplesmentioning

confidence: 99%

Resolving power in liquid chromatography: A trade‐off between efficiency and analysis time

Dores-Sousa

Vos

Eeltink

2018

J of Separation Science

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This review describes chromatographic dispersion and different plate‐height models frequently used to assess the chromatographic performance of ultra‐high‐pressure liquid chromatography column technology. Furthermore, different performance indices, including the resolution, the separation impedance, and kinetic plots are discussed allowing to quantify and visualize the resolving power in liquid chromatography. The construction of kinetic plots is explained, and different visualization approaches are highlighted. Finally, key instrument and column‐technology developments to advance the kinetic performance limits are discussed and selected state‐of‐the‐art applications are highlighted.

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Section: Advancing Performance Limits and Selected Examplesmentioning

confidence: 99%

Resolving power in liquid chromatography: A trade‐off between efficiency and analysis time

Dores-Sousa

Vos

Eeltink

2018

J of Separation Science

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“…There is a good rationale for this as making new custom type of matrix require a hard process of determination of optimal conditions for generation of a suitable support starting from completely new set of monomers. Slight modifications of an existing monolithic matrix material or incorporation of new wanted functionalities within manufacture thereof most commonly, but not exclusively, done through surface modification of prepared monolithic matrix solid phase therefore can lead to a faster support development . Some of the literatures that describe or summarize synthesis of monolithic supports are presented in Table .…”

Section: General Aspects Of Monolithic Materialsmentioning

confidence: 99%

“…This has been exploited to a great extent relying on previously known chemistry of surface modification with reported success in attachment of various small and big molecules. Development of organic polymer based monoliths was initially harder task concerning preparation of material with required structural properties . Fortunately, inherent diversity of organic compounds allowed the utilization of a plethora of co‐monomers to generate materials of equivalent if not better traits .…”

Section: General Aspects Of Monolithic Materialsmentioning

confidence: 99%

Use of monolithic supports for high‐throughput protein and peptide separation in proteomics

2017

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The exclusive properties of monolithic supports enable fast mass transfer, high porosity, low back pressure, easy preparation process and miniaturisation, and the availability of different chemistries make them particularly suitable materials for high-throughput (HTP) protein and peptide separation. In this review recent advances in monolith-based chromatographic supports for HTP screening of protein and peptide samples are presented and their application in HTP sample preparation (separation, enrichment, depletion, proteolytic digestion) for HTP proteomics is discussed. Development and applications of different monolithic capillary columns in HTP MS-based bottom-up and top-down proteomics are overviewed. By discussing the chromatographic conditions and the mass spectrometric data acquisition conditions an attempt is made to present currently demonstrated capacities of monolithic capillary columns for HTP identification and quantification of proteins and peptides from complex biological samples by MS-based proteomics. Some recent advances in basic monolith technology of importance for proteomics are also discussed.

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“…As a result, column designs, in which characteristic size (such as particle diameter) and porosity can be controlled independently, have the potential to yield intrinsically better chromatographic performance. Consequently, polymer–monolithic stationary phases have emerged as attractive alternative for packed columns and find unique applicability in biomolecule analysis .…”

Section: Introductionmentioning

confidence: 99%

Guidelines for tuning the macropore structure of monolithic columns for high‐performance liquid chromatography

Dores-Sousa

Fernández-Pumarega

Vos

et al. 2018

J of Separation Science

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The ability to control the external porosity and to tune the dimensions of the macropore size on multiple length scales provides the possibility of tailoring the monolithic support structure towards separation performance. This paper discusses the properties of conventional polymer–monolithic stationary phases and its limitations regarding the effects of morphology on kinetic performance. Furthermore, guidelines to improve the macropore structure are discussed. The optimal monolithic macropore structure is characterized by high external porosity (while maintaining ultra‐high‐pressure stability), high structure homogeneity, polymer globule clusters in the submicron range, and macropores with a diameter tuned toward speed (small diameter in the 100–500 nm range using short beds) or efficiency (larger macropores in the range of 500 nm–1 μm allowing the use of longer column formats). Finally, promising approaches to control the morphology are discussed.

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Advances in organic polymer-based monolithic column technology for high-resolution liquid chromatography-mass spectrometry profiling of antibodies, intact proteins, oligonucleotides, and peptides

Cited by 74 publications

References 135 publications

Resolving power in liquid chromatography: A trade‐off between efficiency and analysis time

Resolving power in liquid chromatography: A trade‐off between efficiency and analysis time

Use of monolithic supports for high‐throughput protein and peptide separation in proteomics

Guidelines for tuning the macropore structure of monolithic columns for high‐performance liquid chromatography

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