Advances in New Technology for Targeted Modification of Plant Genomes

Zhang, Feng; Puchta, Holger; Thomson, James G.

doi:10.1007/978-1-4939-2556-8

Cited by 19 publications

(1 citation statement)

References 340 publications

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“…The application of ODM in precise genome-editing in models and different crop plants [ 45 ] for metabolic engineering, mode of action, as well as safety regulatory issues were reviewed by Songstad et al [ 44 ]. Among the advancements in the ODM-mediated genome-editing in plants were the development of herbicide tolerance in canola [ 47 ], maize [ 48 ] and tobacco [ 49 ].…”

Section: Genome-editing: Mechanisms and Variant Toolsmentioning

confidence: 99%

Advances in S gene targeted genome-editing and its applicability to disease resistance breeding in selected Solanaceae crop plants

Barka

Lee

2022

Bioengineered

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Genome-editing tools for the development of traits to tolerate abiotic and biotic adversaries are the recently devised breeding techniques revolutionizing molecular breeding by addressing the issues of rapidness and precision. To that end, disease resistance development by disrupting disease susceptibility genes (S genes) to intervene in the biological mechanism of pathogenicity has significantly improved the techniques of molecular breeding. Despite the achievements in genome-editing aimed at the intervention of the function of susceptibility determinants or gene regulatory elements, off-target effects associated with yield-related traits are still the main setbacks. The challenges are attributed to the complexity of the inheritance of traits controlled by pleiotropic genes. Therefore, a more rigorous genome-editing tool with ultra-precision and efficiency for the development of broad-spectrum and durable disease resistance applied to staple crop plants is of critical importance in molecular breeding programs. The main objective of this article is to review the most impressive progresses achieved in resistance breeding against the main diseases of three Solanaceae crops (potato, Solanum tuberosum ; tomato, Solanum lycopersicum and pepper, Capsicum annuum ) using genome-editing by disrupting the sequences of S genes, their promoters, or pathogen genes. In this paper, we discussed the complexity and applicability of genome-editing tools, summarized the main disease of Solanaceae crops, and compiled the recent reports on disease resistance developed by S-gene silencing and their off-target effects. Moreover, GO count and gene annotation were made for pooled S-genes from biological databases. Achievements and prospects of S-gene-based next-generation breeding technologies are also discussed.

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