Advances in Nanocrystallography as a Proteomic Tool

Pechkova, Eugenia; Bragazzi, Nicola Luigi; Nicolini, Claudio

doi:10.1016/b978-0-12-800453-1.00005-1

Cited by 12 publications

(5 citation statements)

References 128 publications

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“…The extremely high ordering of octopus visual membranes, as well as its ability to capture single photons and sensitivity to light polarization [6,9] suggests the possible use of rhodopsins as a prototype for the photonic qubit detectors.…”

Section: Resultsmentioning

confidence: 99%

A Novel Rhodopsin Gene from Octopus vulgaris for Optobioelectronics Materials

Zhgun¹,

Avdanina²,

Eldarov³

et al. 2015

NanoWorld J

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The unique photochromic retinal protein from rhabdomeric octopus membranes -octopus rhodopsin (OctR) has emerged as promising material for biomolecular photonic applications due to its unique properties and advantages. Here we report isolation of the novel full length octR gene from retina cDNA of Octopus vulgaris eyes and its sequence comparison with rhodopsins of other cephalopods and vertebrates. The isolated gene can be used to develop various expression systems for production of recombinant OctR for structural studies and novel optobioelectronic applications. The alignment of amino acid (a.a.) sequence with different opsins revealed similarity to cephalopoda rhodopsins (Rho) and to human melanopsin from intrinsically photosensitive retinal ganglion cells. The alingment of OctR a.a. sequence with mammalian and cephalopoda Rho with known 3D structures revealed promising substitutions V2C and W292C for developing stable and functionally active recombinant OctR after heterologous expression.

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Section: Resultsmentioning

confidence: 99%

A Novel Rhodopsin Gene from Octopus vulgaris for Optobioelectronics Materials

Zhgun¹,

Avdanina²,

Eldarov³

et al. 2015

NanoWorld J

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“…NAPPA microarrays can be useful in biomarkers discovery and for other clinical applications [32,33], especially in the effort of moving towards Personalized Medicine [34][35][36][37][38]. NAPPA can be used also as a sensing system platform enabling the development of biosensor [39].…”

Section: Nappa Technologymentioning

confidence: 99%

NAPPA-Based Vaccines for a New Proteogenomics Approach for Public Health

Bragazzi¹,

Pechkova²,

Nicolini³

2015

NanoWorld J

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Vaccinology was developed for the first time in 1796 when Jenner empirically implemented a vaccine against smallpox using animal-to-human cowpox inoculation. Since then, it has become a very complex science due to the merging of disciplines ranging from structural and functional, cellular and molecular biology and immunology to bioinformatics and nanobiotechnology, as well as systems biology and synthetic biology and engineering. In the frame of evidence-based medicine (EBM), evidence-based vaccinology emerged as an important sub-field: vaccinology has nowadays become more and more predictive and personalized. With the discovery that many patients with cancer develop antibodies against p53 (the so-called oncoantibodies), it was evident that oncoprotein are immunogenic and can be used for immunotherapeutics purposes. In this manuscript, we report Nucleic Acid Programmable Protein Arrays (NAPPA)-based Quartz Crystal Microbalance (QCM) measurement of p53 immunogenicity and kinetics, in the perspective of developing an effective p53 therapy. NAPPA-based QCM_D can be a useful platform for proving the immunogenicity of oncoprotein-based vaccines. Recently, the field of vaccinology has extended from vaccines for infectious diseases to vaccines not only preventive but also therapeutic for chronic-degenerative diseases such as cancer. Peptide-based immunotherapeutics has been proven to be quite effective for cancer treatment and NAPPA-based QCM_D has the promise of providing clinicians with quick, rapid and cheap measurement of oncoprotein kinetics and bindings with immune cells. Moreover, it can be a precious tool for implementing personalized and predictive vaccinology.

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“…However, Langmuir-Blodgett (LB) technique has evolved as efficient tool for organizing nanoscale building blocks of different composition, morphologies, and sizes. Langmuir-Blodgett film can be used as nanobiotemplate for the crystallization of protein which is considered as a major achievement in the field of both structural and functional proteomics [19]. LB based crystallization of proteins provides highly stable protein crystals and allows their characterization at a nano-and subnanoscale dimensions.…”

Section: Journal Of Nanoparticlesmentioning

confidence: 99%

A Simple Approach to Engineer SERS Substrates and Plasmonic Thin Film

Ahamad

Al‐Amin

Ianoul

2014

Journal of Nanoparticles

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Solid supported 2D assembly of silver nanocubes was fabricated by Langmuir-Blodgett technique and employed to investigate its surface enhanced Raman scattering (SERS) and surface enhance fluorescence (SEF) activities by detecting Rh6G in solution of varied concentrations, that is, 10−12 M, 10−9 M, 10−6 M, and 10−3 M. SERS was detected from a nanomolar concentration of Rh6G whereas SEF was detected from a picomolar concentration. Further, the substrate was subjected to thermal annealing to fabricate plasmonic thin film. The formation of thin film was followed by monitoring its surface plasmon resonance spectra and atomic force microscopic images. It was observed that the characteristic spectral peaks of silver nanocubes merged into a broad spectral band as the annealing time was increased and the intensity of the band decreased with the formation of thin film. The obtained result implies that thermal annealing could be a simple approach to create nanoscale gaps in SERS substrate and to engineer continuous thin film from the assembly of discrete nanoparticles.

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Advances in Nanocrystallography as a Proteomic Tool

Cited by 12 publications

References 128 publications

A Novel Rhodopsin Gene from Octopus vulgaris for Optobioelectronics Materials

A Novel Rhodopsin Gene from Octopus vulgaris for Optobioelectronics Materials

NAPPA-Based Vaccines for a New Proteogenomics Approach for Public Health

A Simple Approach to Engineer SERS Substrates and Plasmonic Thin Film

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