Advances in miR-132-Based Biomarker and Therapeutic Potential in the Cardiovascular System

Xu, Kaizu; Chen, Chungui; Wu, Ying; Wu, Mei‐Fang; Lin, Liming

doi:10.3389/fphar.2021.751487

Cited by 16 publications

(6 citation statements)

References 102 publications

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“…The regulatory effect of miR-132 in the oxidative stress pathway in viral infection proposed in our study has yet to be studied. The involvement of miR-132 in oxidative stress has been confirmed in other diseases ( Wasik et al, 2017 ; Zhou et al, 2020 ; Xu et al, 2021 ). Our studies suggested that miR-132 can be involved in tissue damage during L. europaeus infection.…”

Section: Discussionmentioning

confidence: 84%

MicroRNAs participate in the regulation of apoptosis and oxidative stress-related gene expression in rabbits infected with Lagovirus europaeus GI.1 and GI.2 genotypes

Ostrycharz,

Fitzner,

Kęsy

et al. 2024

Front. Microbiol.

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MicroRNAs (miRs) are a group of small, 17–25 nucleotide, non-coding RNA that regulate gene expression at the post-transcriptional level. To date, little is known about the molecular signatures of regulatory interactions between miRs and apoptosis and oxidative stress in viral diseases. Lagovirus europaeus is a virus that causes severe disease in rabbits (Oryctolagus cuniculus) called Rabbit Hemorrhagic Disease (RHD) and belongs to the Caliciviridae family, Lagovirus genus. Within Lagovirus europaeus associated with RHD, two genotypes (GI.1 and GI.2) have been distinguished, and the GI.1 genotype includes four variants (GI.1a, GI.1b, GI.1c, and GI.1d). The study aimed to assess the expression of miRs and their target genes involved in apoptosis and oxidative stress, as well as their potential impact on the pathways during Lagovirus europaeus—two genotypes (GI.1 and GI.2) infection of different virulences in four tissues (liver, lung, kidneys, and spleen). The expression of miRs and target genes related to apoptosis and oxidative stress was determined using quantitative real-time PCR (qPCR). In this study, we evaluated the expression of miR-21 (PTEN, PDCD4), miR-16b (Bcl-2, CXCL10), miR-34a (p53, SIRT1), and miRs—related to oxidative stress—miR-122 (Bach1) and miR-132 (Nfr-2). We also examined the biomarkers of both processes (Bax, Bax/Bcl-2 ratio, Caspase-3, PARP) and HO-I as biomarkers of oxidative stress. Our report is the first to present the regulatory effects of miRs on apoptosis and oxidative stress genes in rabbit infection with Lagovirus europaeus—two genotypes (GI.1 and GI.2) in four tissues (liver, lungs, kidneys, and spleen). The regulatory effect of miRs indicates that, on the one hand, miRs can intensify apoptosis (miR-16b, miR-34a) in the examined organs in response to a viral stimulus and, on the other hand, inhibit (miR-21), which in both cases may be a determinant of the pathogenesis of RHD and tissue damage. Biomarkers of the Bax and Bax/Bcl-2 ratio promote more intense apoptosis after infection with the Lagovirus europaeus GI.2 genotype. Our findings demonstrate that miR-122 and miR-132 regulate oxidative stress in the pathogenesis of RHD, which is associated with tissue damage. The HO-1 biomarker in the course of rabbit hemorrhagic disease indicates oxidative tissue damage. Our findings show that miR-21, miR-16b, and miR-34a regulate three apoptosis pathways. Meanwhile, miR-122 and miR-132 are involved in two oxidative stress pathways.

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Section: Discussionmentioning

confidence: 84%

MicroRNAs participate in the regulation of apoptosis and oxidative stress-related gene expression in rabbits infected with Lagovirus europaeus GI.1 and GI.2 genotypes

Ostrycharz,

Fitzner,

Kęsy

et al. 2024

Front. Microbiol.

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“…There are a number of differences between the time of serum level change and clinical significance. Thus, while miR-1, miR-132, miR-133, and miR-373-3-p suggest the concomitant presence of CAD and Type 2 diabetes, the increase in serum miR-92a levels in diabetic patients occurs independently of coronary artery involvement [112,113]. Furthermore, miR-122-5p and miR-223-3p are upregulated in patients with unstable CAD and may be considered a marker of atherosclerotic plaque vulnerability [114,115].…”

Section: Biomarkers Of Destabilization Of Atherosclerotic Plaque-micr...mentioning

confidence: 99%

Novel Biomarkers of Atherosclerotic Vascular Disease—Latest Insights in the Research Field

Adam¹,

Șalaru

Prisacariu

et al. 2022

IJMS

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The atherosclerotic vascular disease is a cardiovascular continuum in which the main role is attributed to atherosclerosis, from its appearance to its associated complications. The increasing prevalence of cardiovascular risk factors, population ageing, and burden on both the economy and the healthcare system have led to the development of new diagnostic and therapeutic strategies in the field. The better understanding or discovery of new pathophysiological mechanisms and molecules modulating various signaling pathways involved in atherosclerosis have led to the development of potential new biomarkers, with key role in early, subclinical diagnosis. The evolution of technological processes in medicine has shifted the attention of researchers from the profiling of classical risk factors to the identification of new biomarkers such as midregional pro-adrenomedullin, midkine, stromelysin-2, pentraxin 3, inflammasomes, or endothelial cell-derived extracellular vesicles. These molecules are seen as future therapeutic targets associated with decreased morbidity and mortality through early diagnosis of atherosclerotic lesions and future research directions.

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“…The miRNAs discussed in this section are listed in the following Table 3. [250,251] miR-132 Up-regulated I/R injury in mice [259] Hindlimb ischemia in mice [260] TAC-induced cardiac hypertrophy in mice [261,262] FOXO3a [266] SIRT1 [266] PTEN [266] Down-regulated FOXO3a-mediated expression of antioxidant genes such as MnSOD and CAT [103,104] Suppressed deacetylation of FOXO3a, down-regulating antioxidant response [107] Suppressed FOXO-mediated antioxidant gene expression [115] Down-regulated MI in rats [263,264] and mice [265] Up-regulated MI in mice [268] and rats [269] SOD1 [270] Decreased enzymatic removal of superoxide by SOD [240] Down-regulated MI in rats [267] H/R injured CMs [267] miR-214 Up-regulated MI in mice [271,272] and rats [273] I/R injury in mice [274] AAC-induced cardiac hypertrophy in rats [275] 7 IOS induced cardiac fibrosis in rats [278,279] 7 ME2 [271] Decreased NADPH, increased ROS [282] Down-regulated AngII-induced cardiac fibrosis in mice [280] 8 GSR [283] Decreased GSR-mediated conversion of GSSG into GSH [284] 1 Glutamate cysteine ligase catalytic, 2 Glutamate-cystein ligase, 3 Glutathion, 4 Glucose-6-phosphate dehydrogenase, 5 Pentose phosphate pathway, …”

Section: Mir-214mentioning

confidence: 99%

Cardiovascular Disease and miRNAs: Possible Oxidative Stress-Regulating Roles of miRNAs

Lee

2024

Antioxidants

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MicroRNAs (miRNAs) have been highlighted as key players in numerous diseases, and accumulating evidence indicates that pathological expressions of miRNAs contribute to both the development and progression of cardiovascular diseases (CVD), as well. Another important factor affecting the development and progression of CVD is reactive oxygen species (ROS), as well as the oxidative stress they may impose on the cells. Considering miRNAs are involved in virtually every biological process, it is not unreasonable to assume that miRNAs also play critical roles in the regulation of oxidative stress. This narrative review aims to provide mechanistic insights on possible oxidative stress-regulating roles of miRNAs in cardiovascular diseases based on differentially expressed miRNAs reported in various cardiovascular diseases and their empirically validated targets that have been implicated in the regulation of oxidative stress.

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Advances in miR-132-Based Biomarker and Therapeutic Potential in the Cardiovascular System

Cited by 16 publications

References 102 publications

MicroRNAs participate in the regulation of apoptosis and oxidative stress-related gene expression in rabbits infected with Lagovirus europaeus GI.1 and GI.2 genotypes

MicroRNAs participate in the regulation of apoptosis and oxidative stress-related gene expression in rabbits infected with Lagovirus europaeus GI.1 and GI.2 genotypes

Novel Biomarkers of Atherosclerotic Vascular Disease—Latest Insights in the Research Field

Cardiovascular Disease and miRNAs: Possible Oxidative Stress-Regulating Roles of miRNAs

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